Search Results
Overview
| Uniprot ID | P61327 |
|---|---|
| Protein Name | Protein mago nashi homolog |
| Gene Name | Magoh |
| Organism | Mus musculus |
Kla Sites from experimental identification
| Position | Flanking peptide |
|---|---|
| 16 | YYVGHKGKFGHEFLE |
| 41 | YANNSNYKNDVMIRK |
Function
Required for pre-mRNA splicing as component of the spliceosome. Plays a redundant role with MAGOHB as core component of the exon junction complex (EJC) and in the nonsense-mediated decay (NMD) pathway. The EJC is a dynamic structure consisting of core proteins and several peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. The EJC marks the position of the exon-exon junction in the mature mRNA for the gene expression machinery and the core components remain bound to spliced mRNAs throughout all stages of mRNA metabolism thereby influencing downstream processes including nuclear mRNA export, subcellular mRNA localization, translation efficiency and nonsense-mediated mRNA decay (NMD). The MAGOH-RBM8A heterodimer inhibits the ATPase activity of EIF4A3, thereby trapping the ATP-bound EJC core onto spliced mRNA in a stable conformation. The MAGOH-RBM8A heterodimer interacts with the EJC key regulator PYM1 leading to EJC disassembly in the cytoplasm and translation enhancement of EJC-bearing spliced mRNAs by recruiting them to the ribosomal 48S pre-initiation complex. Involved in the splicing modulation of BCL2L1/Bcl-X (and probably other apoptotic genes); specifically inhibits formation of proapoptotic isoforms; the function is different from the established EJC assembly
Protein Sequence
Gene Ontology
| Classification | GO ID | Description |
|---|---|---|
| Cellular Component | GO:0071013 | catalytic step 2 spliceosome |
| Cellular Component | GO:0005829 | cytosol |
| Cellular Component | GO:0035145 | exon-exon junction complex |
| Cellular Component | GO:1990501 | exon-exon junction subcomplex mago-y14 |
| Cellular Component | GO:0016607 | nuclear speck |
| Cellular Component | GO:0005654 | nucleoplasm |
| Cellular Component | GO:0005634 | nucleus |
| Cellular Component | GO:0071020 | post-spliceosomal complex |
| Cellular Component | GO:0005681 | spliceosomal complex |
| Cellular Component | GO:0071006 | U2-type catalytic step 1 spliceosome |
| Cellular Component | GO:0071007 | U2-type catalytic step 2 spliceosome |
| Molecular Function | GO:0003723 | RNA binding |
| Biological Process | GO:0007292 | female gamete generation |
| Biological Process | GO:0006406 | mRNA export from nucleus |
| Biological Process | GO:0016071 | mRNA metabolic process |
| Biological Process | GO:0000398 | mRNA splicing, via spliceosome |
| Biological Process | GO:0000184 | nuclear-transcribed mRNA catabolic process, nonsense-mediated decay |
| Biological Process | GO:0000381 | regulation of alternative mRNA splicing, via spliceosome |
| Biological Process | GO:0050684 | regulation of mRNA processing |
| Biological Process | GO:2000622 | regulation of nuclear-transcribed mRNA catabolic process, nonsense-mediated decay |
| Biological Process | GO:0006417 | regulation of translation |
| Biological Process | GO:0008380 | RNA splicing |
Reference
[1] Sung E, Sim H, Cho YC, Lee W, Bae JS et al.. Global Profiling of Lysine Acetylation and Lactylation in Kupffer Cells.. J Proteome Res 22(12):3683-3691. 2023 Dec 1. PMID: 37897433.
[2] Wu D, Tang Y, Li X, Xiong S, Zhang Z et al.. Characterization of protein lactylation in healthy and ischemic mouse hearts.. Front Cardiovasc Med 12:1644886. 2025. PMID: 41089239.