Overview
| Uniprot ID | P61621 |
|---|
| Protein Name | Protein transport protein Sec61 subunit alpha isoform 1 |
|---|
| Gene Name | Sec61a1 |
|---|
| Organism | Rattus norvegicus |
|---|
Kla Sites from experimental identification
| Position |
Flanking peptide |
| 107 |
IEVGDTPKDRALFNG |
Function
Component of SEC61 channel-forming translocon complex that mediates transport of signal peptide-containing precursor polypeptides across the endoplasmic reticulum (ER). Forms a ribosome receptor and a gated pore in the ER membrane, both functions required for cotranslational translocation of nascent polypeptides. May cooperate with auxiliary protein SEC62, SEC63 and HSPA5/BiP to enable post-translational transport of small presecretory proteins. The SEC61 channel is also involved in ER membrane insertion of transmembrane proteins: it mediates membrane insertion of the first few transmembrane segments of proteins, while insertion of subsequent transmembrane regions of multi-pass membrane proteins is mediated by the multi-pass translocon (MPT) complex. The SEC61 channel cooperates with the translocating protein TRAM1 to import nascent proteins into the ER. Controls the passive efflux of calcium ions from the ER lumen to the cytosol through SEC61 channel, contributing to the maintenance of cellular calcium homeostasis (By similarity). Plays a critical role in nephrogenesis, specifically at pronephros stage (By similarity)
Protein Sequence
10
MAIKFLEVIK
20
PFCVILPEIQ
30
KPERKIQFKE
40
KVLWTAITLF
50
IFLVCCQIPL
60
FGIMSSDSAD
70
PFYWMRVILA
80
SNRGTLMELG
90
ISPIVTSGLI
100
MQLLAGAKII
110
EVGDTPKDRA
120
LFNGAQKLFG
130
MIITIGQSIV
140
YVMTGMYGDP
150
SEMGAGICLL
160
ITIQLFVAGL
170
IVLLLDELLQ
180
KGYGLGSGIS
190
LFIATNICET
200
IVWKAFSPTT
210
VNTGRGMEFE
220
GAIIALFHLL
230
ATRTDKVRAL
240
REAFYRQNLP
250
NLMNLIATIF
260
VFAVVIYFQG
270
FRVDLPIKSA
280
RYRGQYNTYP
290
IKLFYTSNIP
300
IILQSALVSN
310
LYVISQMLSA
320
RFSGNLLVSL
330
LGTWSDTSSG
340
GPARAYPVGG
350
LCYYLSPPES
360
FGSVLEDPVH
370
AVVYIVFMLG
380
SCAFFSKTWI
390
EVSGSSAKDV
400
AKQLKEQQMV
410
MRGHRETSMV
420
HELNRYIPTA
430
AAFGGLCIGA
440
LSVLADFLGA
450
IGSGTGILLA
460
VTIIYQYFEI
470
FVKEQSEVGS
MGALLF
Gene Ontology
| Classification |
GO ID |
Description |
| Cellular Component |
GO:0005783 |
endoplasmic reticulum |
| Cellular Component |
GO:0005789 |
endoplasmic reticulum membrane |
| Cellular Component |
GO:0016020 |
membrane |
| Cellular Component |
GO:0005784 |
Sec61 translocon complex |
| Molecular Function |
GO:0005262 |
calcium channel activity |
| Molecular Function |
GO:0008320 |
protein transmembrane transporter activity |
| Molecular Function |
GO:0043022 |
ribosome binding |
| Molecular Function |
GO:0005048 |
signal sequence binding |
| Molecular Function |
GO:0022857 |
transmembrane transporter activity |
| Biological Process |
GO:0006613 |
cotranslational protein targeting to membrane |
| Biological Process |
GO:0007029 |
endoplasmic reticulum organization |
| Biological Process |
GO:0006620 |
post-translational protein targeting to endoplasmic reticulum membrane |
| Biological Process |
GO:0031204 |
post-translational protein targeting to membrane, translocation |
| Biological Process |
GO:0039019 |
pronephric nephron development |
| Biological Process |
GO:0045048 |
protein insertion into ER membrane |
| Biological Process |
GO:0045047 |
protein targeting to ER |
| Biological Process |
GO:0034341 |
response to type II interferon |
| Biological Process |
GO:0006614 |
SRP-dependent cotranslational protein targeting to membrane |
| Biological Process |
GO:0006616 |
SRP-dependent cotranslational protein targeting to membrane, translocation |
Reference
[1] Sheng L, Xu H, Wang Y, Ni J, Xiang T et al.. Systematic analysis of lysine lactylation in nucleus pulposus cells.. iScience 27(11):111157. 2024 Nov 15. PMID: 39524337.