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Overview

Uniprot IDP62141
Protein NameSerine/threonine-protein phosphatase PP1-beta catalytic subunit
Gene NamePpp1cb
OrganismMus musculus

Kla Sites from experimental identification

Position Flanking peptide
25 VRGCRPGKIVQMTEA

Function

Protein phosphatase that associates with over 200 regulatory proteins to form highly specific holoenzymes which dephosphorylate hundreds of biological targets. Protein phosphatase (PP1) is essential for cell division, it participates in the regulation of glycogen metabolism, muscle contractility and protein synthesis. Involved in regulation of ionic conductances and long-term synaptic plasticity. Component of the PTW/PP1 phosphatase complex, which plays a role in the control of chromatin structure and cell cycle progression during the transition from mitosis into interphase. In balance with CSNK1D and CSNK1E, determines the circadian period length, through the regulation of the speed and rhythmicity of PER1 and PER2 phosphorylation. May dephosphorylate CSNK1D and CSNK1E. Core component of the SHOC2-MRAS-PP1c (SMP) holophosphatase complex that regulates the MAPK pathway activation (By similarity). The SMP complex specifically dephosphorylates the inhibitory phosphorylation at 'Ser-259' of RAF1 kinase, 'Ser-365' of BRAF kinase and 'Ser-214' of ARAF kinase, stimulating their kinase activities (By similarity). The SMP complex enhances the dephosphorylation activity and substrate specificity of PP1c (By similarity)

Protein Sequence

10 MADGELNVDS 20 LITRLLEVRG 30 CRPGKIVQMT 40 EAEVRGLCIK 50 SREIFLSQPI 60 LLELEAPLKI 70 CGDIHGQYTD 80 LLRLFEYGGF 90 PPEANYLFLG 100 DYVDRGKQSL 110 ETICLLLAYK 120 IKYPENFFLL 130 RGNHECASIN 140 RIYGFYDECK 150 RRFNIKLWKT 160 FTDCFNCLPI 170 AAIVDEKIFC 180 CHGGLSPDLQ 190 SMEQIRRIMR 200 PTDVPDTGLL 210 CDLLWSDPDK 220 DVQGWGENDR 230 GVSFTFGADV 240 VSKFLNRHDL 250 DLICRAHQVV 260 EDGYEFFAKR 270 QLVTLFSAPN 280 YCGEFDNAGG 290 MMSVDETLMC 300 SFQILKPSEK 310 KAKYQYGGLN 320 SGRPVTPPRT ANPPKKR

Gene Ontology

Classification GO ID Description
Cellular Component GO:0000781 chromosome, telomeric region
Cellular Component GO:0005737 cytoplasm
Cellular Component GO:0005829 cytosol
Cellular Component GO:0042587 glycogen granule
Cellular Component GO:0005730 nucleolus
Cellular Component GO:0005654 nucleoplasm
Cellular Component GO:0005634 nucleus
Cellular Component GO:0000164 protein phosphatase type 1 complex
Cellular Component GO:0072357 PTW/PP1 phosphatase complex
Molecular Function GO:0046872 metal ion binding
Molecular Function GO:0017018 myosin phosphatase activity
Molecular Function GO:0050115 myosin-light-chain-phosphatase activity
Molecular Function GO:0016791 phosphatase activity
Molecular Function GO:0004721 phosphoprotein phosphatase activity
Molecular Function GO:0019901 protein kinase binding
Molecular Function GO:0004722 protein serine/threonine phosphatase activity
Biological Process GO:0051301 cell division
Biological Process GO:0032922 circadian regulation of gene expression
Biological Process GO:0043153 entrainment of circadian clock by photoperiod
Biological Process GO:0005977 glycogen metabolic process
Biological Process GO:0006470 protein dephosphorylation
Biological Process GO:0030155 regulation of cell adhesion
Biological Process GO:0042752 regulation of circadian rhythm
Biological Process GO:0005979 regulation of glycogen biosynthetic process
Biological Process GO:0005981 regulation of glycogen catabolic process

Reference

[1] Chang J, Wu W, Qian P, Lu Z, He X et al.. Multi-omics study on the effect of moderate-intensity exercise on protein lactylation in mouse muscle tissue.. Front Cell Dev Biol 12:1472338. 2024. PMID: 39935788.

[2] Wu D, Tang Y, Li X, Xiong S, Zhang Z et al.. Characterization of protein lactylation in healthy and ischemic mouse hearts.. Front Cardiovasc Med 12:1644886. 2025. PMID: 41089239.