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Overview

Uniprot IDP62495
Protein NameEukaryotic peptide chain release factor subunit 1
Gene NameETF1
OrganismHomo sapiens

Kla Sites from experimental identification

Position Flanking peptide
178 KFTVDLPKKHGRGGQ
179 FTVDLPKKHGRGGQS
249 FDQRLQSKVLKLVDI

Function

Component of the eRF1-eRF3-GTP ternary complex, a ternary complex that mediates translation termination in response to the termination codons (PubMed:10676813, PubMed:16777602, PubMed:24486019, PubMed:26245381, PubMed:27863242, PubMed:36638793, PubMed:7990965). The eRF1-eRF3-GTP complex binds to a stop codon in the ribosomal A-site (PubMed:26245381, PubMed:27863242, PubMed:36638793). ETF1/ERF1 is responsible for stop codon recognition and inducing hydrolysis of peptidyl-tRNA (PubMed:26245381, PubMed:27863242, PubMed:36638793). Following GTP hydrolysis, eRF3 (GSPT1/ERF3A or GSPT2/ERF3B) dissociates, permitting ETF1/eRF1 to accommodate fully in the A-site and mediate hydrolysis of peptidyl-tRNA (PubMed:10676813, PubMed:16777602, PubMed:26245381, PubMed:27863242). Component of the transient SURF complex which recruits UPF1 to stalled ribosomes in the context of nonsense-mediated decay (NMD) of mRNAs containing premature stop codons (PubMed:19417104). Required for SHFL-mediated translation termination which inhibits programmed ribosomal frameshifting (-1PRF) of mRNA from viruses and cellular genes (PubMed:30682371)

Protein Sequence

10 MADDPSAADR 20 NVEIWKIKKL 30 IKSLEAARGN 40 GTSMISLIIP 50 PKDQISRVAK 60 MLADEFGTAS 70 NIKSRVNRLS 80 VLGAITSVQQ 90 RLKLYNKVPP 100 NGLVVYCGTI 110 VTEEGKEKKV 120 NIDFEPFKPI 130 NTSLYLCDNK 140 FHTEALTALL 150 SDDSKFGFIV 160 IDGSGALFGT 170 LQGNTREVLH 180 KFTVDLPKKH 190 GRGGQSALRF 200 ARLRMEKRHN 210 YVRKVAETAV 220 QLFISGDKVN 230 VAGLVLAGSA 240 DFKTELSQSD 250 MFDQRLQSKV 260 LKLVDISYGG 270 ENGFNQAIEL 280 STEVLSNVKF 290 IQEKKLIGRY 300 FDEISQDTGK 310 YCFGVEDTLK 320 ALEMGAVEIL 330 IVYENLDIMR 340 YVLHCQGTEE 350 EKILYLTPEQ 360 EKDKSHFTDK 370 ETGQEHELIE 380 SMPLLEWFAN 390 NYKKFGATLE 400 IVTDKSQEGS 410 QFVKGFGGIG 420 GILRYRVDFQ 430 GMEYQGGDDE FFDLDDY

Gene Ontology

Classification GO ID Description
Cellular Component GO:0005737 cytoplasm
Cellular Component GO:0005829 cytosol
Cellular Component GO:0022626 cytosolic ribosome
Cellular Component GO:0018444 translation release factor complex
Molecular Function GO:0004045 peptidyl-tRNA hydrolase activity
Molecular Function GO:0043022 ribosome binding
Molecular Function GO:0003723 RNA binding
Molecular Function GO:1990825 sequence-specific mRNA binding
Molecular Function GO:0003747 translation release factor activity
Molecular Function GO:0016149 translation release factor activity, codon specific
Molecular Function GO:0008079 translation termination factor activity
Biological Process GO:0002184 cytoplasmic translational termination
Biological Process GO:0000184 nuclear-transcribed mRNA catabolic process, nonsense-mediated decay
Biological Process GO:0006479 protein methylation
Biological Process GO:0006449 regulation of translational termination
Biological Process GO:0006415 translational termination

Reference

[1] Yang D, Yin J, Shan L, Yi X, Zhang W et al.. Identification of lysine-lactylated substrates in gastric cancer cells.. iScience 25(7):104630. 2022 Jul 15. PMID: 35800753.

[2] Wu Q, Li Z, Gong T, Zheng X, Zhou X et al.. Porphyromonas gingivalis infection induces lysine lactylation reprogramming in human umbilical vein endothelial cells.. Front Cell Infect Microbiol 16:1706727. 2026. PMID: 41696360.