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Overview

Uniprot IDP62630
Protein NameElongation factor 1-alpha 1
Gene NameEef1a1
OrganismRattus norvegicus

Kla Sites from experimental identification

Position Flanking peptide
154 QLIVGVNKMDSTEPP
165 TEPPYSQKRYEEIVK
172 KRYEEIVKEVSTYIK
179 KEVSTYIKKIGYNPD
219 KGWKVTRKDGSASGT
255 LPLQDVYKIGGIGTV
273 RVETGVLKPGMVVTF
386 IDRRSGKKLEDGPKF
392 KKLEDGPKFLKSGDA
395 EDGPKFLKSGDAAIV
41 IDKRTIEKFEKEAAE
439 TVAVGVIKAVDKKAA
44 RTIEKFEKEAAEMGK
450 KKAAGAGKVTKSAQK
453 AGAGKVTKSAQKAQK
5 ***MGKEKTHINIVV

Function

Translation elongation factor that catalyzes the GTP-dependent binding of aminoacyl-tRNA (aa-tRNA) to the A-site of ribosomes during the elongation phase of protein synthesis. Base pairing between the mRNA codon and the aa-tRNA anticodon promotes GTP hydrolysis, releasing the aa-tRNA from EEF1A1 and allowing its accommodation into the ribosome. The growing protein chain is subsequently transferred from the P-site peptidyl tRNA to the A-site aa-tRNA, extending it by one amino acid through ribosome-catalyzed peptide bond formation. Also plays a role in the positive regulation of IFNG transcription in T-helper 1 cells as part of an IFNG promoter-binding complex with TXK and PARP1 (By similarity). Also plays a role in cytoskeleton organization by promoting actin bundling (By similarity)

Protein Sequence

10 MGKEKTHINI 20 VVIGHVDSGK 30 STTTGHLIYK 40 CGGIDKRTIE 50 KFEKEAAEMG 60 KGSFKYAWVL 70 DKLKAERERG 80 ITIDISLWKF 90 ETSKYYVTII 100 DAPGHRDFIK 110 NMITGTSQAD 120 CAVLIVAAGV 130 GEFEAGISKN 140 GQTREHALLA 150 YTLGVKQLIV 160 GVNKMDSTEP 170 PYSQKRYEEI 180 VKEVSTYIKK 190 IGYNPDTVAF 200 VPISGWNGDN 210 MLEPSANMPW 220 FKGWKVTRKD 230 GSASGTTLLE 240 ALDCILPPTR 250 PTDKPLRLPL 260 QDVYKIGGIG 270 TVPVGRVETG 280 VLKPGMVVTF 290 APVNVTTEVK 300 SVEMHHEALS 310 EALPGDNVGF 320 NVKNVSVKDV 330 RRGNVAGDSK 340 NDPPMEAAGF 350 TAQVIILNHP 360 GQISAGYAPV 370 LDCHTAHIAC 380 KFAELKEKID 390 RRSGKKLEDG 400 PKFLKSGDAA 410 IVDMVPGKPM 420 CVESFSDYPP 430 LGRFAVRDMR 440 QTVAVGVIKA 450 VDKKAAGAGK 460 VTKSAQKAQK AK

Gene Ontology

Classification GO ID Description
Cellular Component GO:0030864 cortical actin cytoskeleton
Molecular Function GO:0019900 kinase binding
Molecular Function GO:0060090 molecular adaptor activity
Molecular Function GO:0003729 mRNA binding
Molecular Function GO:0019901 protein kinase binding
Molecular Function GO:0003746 translation elongation factor activity
Molecular Function GO:0000049 tRNA binding
Biological Process GO:0071364 cellular response to epidermal growth factor stimulus
Biological Process GO:1990090 cellular response to nerve growth factor stimulus
Biological Process GO:0071466 cellular response to xenobiotic stimulus
Biological Process GO:1903427 negative regulation of reactive oxygen species biosynthetic process
Biological Process GO:0043065 positive regulation of apoptotic process
Biological Process GO:0010976 positive regulation of neuron projection development
Biological Process GO:0099150 regulation of postsynaptic specialization assembly
Biological Process GO:0006412 translation
Biological Process GO:0006414 translational elongation
Cellular Component GO:0005737 cytoplasm
Cellular Component GO:0005829 cytosol
Cellular Component GO:0022626 cytosolic ribosome
Cellular Component GO:0098982 GABA-ergic synapse
Cellular Component GO:0005765 lysosomal membrane
Cellular Component GO:0005730 nucleolus
Cellular Component GO:0005634 nucleus
Cellular Component GO:0005886 plasma membrane
Cellular Component GO:0098794 postsynapse
Cellular Component GO:0005840 ribosome
Cellular Component GO:0032587 ruffle membrane
Molecular Function GO:0005516 calmodulin binding
Molecular Function GO:0005525 GTP binding
Molecular Function GO:0003924 GTPase activity
Molecular Function GO:1901363 heterocyclic compound binding
Molecular Function GO:0042802 identical protein binding
Molecular Function GO:0019209 kinase activator activity

Reference

[1] Sheng L, Xu H, Wang Y, Ni J, Xiang T et al.. Systematic analysis of lysine lactylation in nucleus pulposus cells.. iScience 27(11):111157. 2024 Nov 15. PMID: 39524337.

[2] Chen Y, Sun W, Sun Z, Zhao H, Wu T et al.. Effect of electroacupuncture on hippocampal protein lactylation in a rat model of vascular dementia.. Front Neurol 16:1629474. 2025. PMID: 40963935.