Overview
| Uniprot ID | P62744 |
|---|
| Protein Name | AP-2 complex subunit sigma |
|---|
| Gene Name | Ap2s1 |
|---|
| Organism | Rattus norvegicus |
|---|
Kla Sites from experimental identification
| Position |
Flanking peptide |
| 45 |
VVTVRDAKHTNFVEF |
Function
Component of the adaptor protein complex 2 (AP-2). Adaptor protein complexes function in protein Transport via Transport vesicles in different membrane traffic pathways. Adaptor protein complexes are vesicle coat components and appear to be involved in cargo selection and vesicle formation. AP-2 is involved in clathrin-dependent endocytosis in which cargo proteins are incorporated into vesicles surrounded by clathrin (clathrin-coated vesicles, CCVs) which are destined for fusion with the early endosome. The clathrin lattice serves as a mechanical scaffold but is itself unable to bind directly to membrane components. Clathrin-associated adaptor protein (AP) complexes which can bind directly to both the clathrin lattice and to the lipid and protein components of membranes are considered to be the major clathrin adaptors contributing the CCV formation. AP-2 also serves as a cargo receptor to selectively sort the membrane proteins involved in receptor-mediated endocytosis. AP-2 seems to play a role in the recycling of synaptic vesicle membranes from the presynaptic surface. AP-2 recognizes Y-X-X-[FILMV] (Y-X-X-Phi) and [ED]-X-X-X-L-[LI] endocytosis signal motifs within the cytosolic tails of transmembrane cargo molecules. AP-2 may also play a role in maintaining normal post-endocytic trafficking through the ARF6-regulated, non-clathrin pathway. The AP-2 alpha and AP-2 sigma subunits are thought to contribute to the recognition of the [ED]-X-X-X-L-[LI] motif. May also play a role in extracellular calcium homeostasis (By similarity)
Protein Sequence
10
MIRFILIQNR
20
AGKTRLAKWY
30
MQFDDDEKQK
40
LIEEVHAVVT
50
VRDAKHTNFV
60
EFRNFKIIYR
70
RYAGLYFCIC
80
VDVNDNNLAY
90
LEAIHNFVEV
100
LNEYFHNVCE
110
LDLVFNFYKV
120
YTVVDEMFLA
130
GEIRETSQTK
140
VLKQLLMLQS
LE
Gene Ontology
| Classification |
GO ID |
Description |
| Cellular Component |
GO:0030122 |
AP-2 adaptor complex |
| Cellular Component |
GO:0098894 |
extrinsic component of presynaptic endocytic zone membrane |
| Cellular Component |
GO:0098978 |
glutamatergic synapse |
| Cellular Component |
GO:0005886 |
plasma membrane |
| Cellular Component |
GO:0098794 |
postsynapse |
| Cellular Component |
GO:0045202 |
synapse |
| Cellular Component |
GO:0008021 |
synaptic vesicle |
| Molecular Function |
GO:0035615 |
clathrin adaptor activity |
| Biological Process |
GO:0072583 |
clathrin-dependent endocytosis |
| Biological Process |
GO:0006886 |
intracellular protein transport |
| Biological Process |
GO:0098884 |
postsynaptic neurotransmitter receptor internalization |
| Biological Process |
GO:0048488 |
synaptic vesicle endocytosis |
| Biological Process |
GO:0016192 |
vesicle-mediated transport |
Reference
[1] Chen Y, Sun W, Sun Z, Zhao H, Wu T et al.. Effect of electroacupuncture on hippocampal protein lactylation in a rat model of vascular dementia.. Front Neurol 16:1629474. 2025. PMID: 40963935.