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Overview

Uniprot IDP62827
Protein NameGTP-binding nuclear protein Ran
Gene NameRan
OrganismMus musculus

Kla Sites from experimental identification

Position Flanking peptide
134 KDRKVKAKSIVFHRK

Function

GTPase involved in nucleocytoplasmic transport, participating both to the import and the export from the nucleus of proteins and RNAs. Switches between a cytoplasmic GDP- and a nuclear GTP-bound state by nucleotide exchange and GTP hydrolysis. Nuclear import receptors such as importin beta bind their substrates only in the absence of GTP-bound RAN and release them upon direct interaction with GTP-bound RAN, while export receptors behave in the opposite way. Thereby, RAN controls cargo loading and release by transport receptors in the proper compartment and ensures the directionality of the transport. Interaction with RANBP1 induces a conformation change in the complex formed by XPO1 and RAN that triggers the release of the nuclear export signal of cargo proteins. RAN (GTP-bound form) triggers microtubule assembly at mitotic chromosomes and is required for normal mitotic spindle assembly and chromosome segregation. Required for normal progress through mitosis. The complex with BIRC5/survivin plays a role in mitotic spindle formation by serving as a physical scaffold to help deliver the RAN effector molecule TPX2 to microtubules. Acts as a negative regulator of the kinase activity of VRK1 and VRK2. Enhances AR-mediated transactivation

Protein Sequence

10 MAAQGEPQVQ 20 FKLVLVGDGG 30 TGKTTFVKRH 40 LTGEFEKKYV 50 ATLGVEVHPL 60 VFHTNRGPIK 70 FNVWDTAGQE 80 KFGGLRDGYY 90 IQAQCAIIMF 100 DVTSRVTYKN 110 VPNWHRDLVR 120 VCENIPIVLC 130 GNKVDIKDRK 140 VKAKSIVFHR 150 KKNLQYYDIS 160 AKSNYNFEKP 170 FLWLARKLIG 180 DPNLEFVAMP 190 ALAPPEVVMD 200 PALAAQYEHD 210 LEVAQTTALP DEDDDL

Gene Ontology

Classification GO ID Description
Cellular Component GO:0036126 sperm flagellum
Cellular Component GO:0005814 centriole
Cellular Component GO:0000785 chromatin
Cellular Component GO:0005737 cytoplasm
Cellular Component GO:0005829 cytosol
Cellular Component GO:0001673 male germ cell nucleus
Cellular Component GO:0002177 manchette
Cellular Component GO:0042470 melanosome
Cellular Component GO:0030496 midbody
Cellular Component GO:0005635 nuclear envelope
Cellular Component GO:0005643 nuclear pore
Cellular Component GO:0005730 nucleolus
Cellular Component GO:0005654 nucleoplasm
Cellular Component GO:0005634 nucleus
Cellular Component GO:0032991 protein-containing complex
Cellular Component GO:0055037 recycling endosome
Cellular Component GO:0042565 RNA nuclear export complex
Molecular Function GO:0045505 dynein intermediate chain binding
Molecular Function GO:0003925 G protein activity
Molecular Function GO:0019003 GDP binding
Molecular Function GO:0005525 GTP binding
Molecular Function GO:0003924 GTPase activity
Molecular Function GO:0061676 importin-alpha family protein binding
Molecular Function GO:0000287 magnesium ion binding
Molecular Function GO:0070883 pre-miRNA binding
Molecular Function GO:0019904 protein domain specific binding
Molecular Function GO:0046982 protein heterodimerization activity
Molecular Function GO:0044877 protein-containing complex binding
Biological Process GO:0030036 actin cytoskeleton organization
Biological Process GO:0051301 cell division
Biological Process GO:0071389 cellular response to mineralocorticoid stimulus
Biological Process GO:0006096 glycolytic process
Biological Process GO:0046039 GTP metabolic process
Biological Process GO:0021766 hippocampus development
Biological Process GO:0008104 intracellular protein localization
Biological Process GO:0000070 mitotic sister chromatid segregation
Biological Process GO:0042307 positive regulation of protein import into nucleus
Biological Process GO:0006611 protein export from nucleus
Biological Process GO:0006606 protein import into nucleus
Biological Process GO:1902570 protein localization to nucleolus
Biological Process GO:0031503 protein-containing complex localization
Biological Process GO:0000055 ribosomal large subunit export from nucleus
Biological Process GO:0000056 ribosomal small subunit export from nucleus
Biological Process GO:0000054 ribosomal subunit export from nucleus
Biological Process GO:0061015 snRNA import into nucleus
Biological Process GO:0007286 spermatid development

Reference

[1] Sung E, Sim H, Cho YC, Lee W, Bae JS et al.. Global Profiling of Lysine Acetylation and Lactylation in Kupffer Cells.. J Proteome Res 22(12):3683-3691. 2023 Dec 1. PMID: 37897433.

[2] Wu D, Tang Y, Li X, Xiong S, Zhang Z et al.. Characterization of protein lactylation in healthy and ischemic mouse hearts.. Front Cardiovasc Med 12:1644886. 2025. PMID: 41089239.