Search Results
Overview
| Uniprot ID | P62909 |
|---|---|
| Protein Name | Small ribosomal subunit protein uS3 |
| Gene Name | Rps3 |
| Organism | Rattus norvegicus |
Kla Sites from experimental identification
| Position | Flanking peptide |
|---|---|
| 10 | VQISKKRKFVADGIF |
| 62 | TQNVLGEKGRRIREL |
| 75 | ELTAVVQKRFGFPEG |
Function
Component of the small ribosomal subunit (By similarity). The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell (By similarity). Has endonuclease activity and plays a role in repair of damaged DNA (PubMed:7775413). Cleaves phosphodiester bonds of DNAs containing altered bases with broad specificity and cleaves supercoiled DNA more efficiently than relaxed DNA (By similarity). Displays high binding affinity for 7,8-dihydro-8-oxoguanine (8-oxoG), a common DNA lesion caused by reactive oxygen species (ROS) (By similarity). Has also been shown to bind with similar affinity to intact and damaged DNA (By similarity). Stimulates the N-glycosylase activity of the base excision protein OGG1 (By similarity). Enhances the uracil excision activity of UNG1 (By similarity). Also stimulates the cleavage of the phosphodiester backbone by APEX1 (By similarity). When located in the mitochondrion, reduces cellular ROS levels and mitochondrial DNA damage. Has also been shown to negatively regulate DNA repair in cells exposed to hydrogen peroxide (By similarity). Plays a role in regulating transcription as part of the NF-kappa-B p65-p50 complex where it binds to the RELA/p65 subunit, enhances binding of the complex to DNA and promotes transcription of target genes (By similarity). Represses its own translation by binding to its cognate mRNA (By similarity). Binds to and protects TP53/p53 from MDM2-mediated ubiquitination (By similarity). Involved in spindle formation and chromosome movement during mitosis by regulating microtubule polymerization (By similarity). Involved in induction of apoptosis through its role in activation of CASP8 (By similarity). Induces neuronal apoptosis by interacting with the E2F1 transcription factor and acting synergistically with it to up-regulate pro-apoptotic proteins BCL2L11/BIM and HRK/Dp5 (By similarity). Interacts with TRADD following exposure to UV radiation and induces apoptosis by caspase-dependent JNK activation (By similarity)
Protein Sequence
Gene Ontology
| Classification | GO ID | Description |
|---|---|---|
| Cellular Component | GO:0005737 | cytoplasm |
| Cellular Component | GO:0071159 | NF-kappaB complex |
| Cellular Component | GO:0005730 | nucleolus |
| Cellular Component | GO:0005634 | nucleus |
| Cellular Component | GO:0005886 | plasma membrane |
| Cellular Component | GO:0014069 | postsynaptic density |
| Cellular Component | GO:1990904 | ribonucleoprotein complex |
| Cellular Component | GO:0005840 | ribosome |
| Cellular Component | GO:0032587 | ruffle membrane |
| Cellular Component | GO:0045202 | synapse |
| Molecular Function | GO:0140078 | class I DNA-(apurinic or apyrimidinic site) endonuclease activity |
| Molecular Function | GO:0003684 | damaged DNA binding |
| Molecular Function | GO:0003677 | DNA binding |
| Molecular Function | GO:0004520 | DNA endonuclease activity |
| Molecular Function | GO:0003906 | DNA-(apurinic or apyrimidinic site) endonuclease activity |
| Molecular Function | GO:0001228 | DNA-binding transcription activator activity, RNA polymerase II-specific |
| Molecular Function | GO:0140297 | DNA-binding transcription factor binding |
| Molecular Function | GO:0019899 | enzyme binding |
| Molecular Function | GO:0030544 | Hsp70 protein binding |
| Molecular Function | GO:0051879 | Hsp90 protein binding |
| Molecular Function | GO:0019900 | kinase binding |
| Molecular Function | GO:0008017 | microtubule binding |
| Molecular Function | GO:0003729 | mRNA binding |
| Molecular Function | GO:0032357 | oxidized purine DNA binding |
| Molecular Function | GO:0032358 | oxidized pyrimidine DNA binding |
| Molecular Function | GO:0051018 | protein kinase A binding |
| Molecular Function | GO:0019901 | protein kinase binding |
| Molecular Function | GO:0003723 | RNA binding |
| Molecular Function | GO:0070181 | small ribosomal subunit rRNA binding |
| Molecular Function | GO:0003735 | structural constituent of ribosome |
| Molecular Function | GO:0097100 | supercoiled DNA binding |
| Molecular Function | GO:0015631 | tubulin binding |
| Molecular Function | GO:0044390 | ubiquitin-like protein conjugating enzyme binding |
| Biological Process | GO:0006915 | apoptotic process |
| Biological Process | GO:0006284 | base-excision repair |
| Biological Process | GO:0051301 | cell division |
| Biological Process | GO:0070301 | cellular response to hydrogen peroxide |
| Biological Process | GO:1990090 | cellular response to nerve growth factor stimulus |
| Biological Process | GO:0034614 | cellular response to reactive oxygen species |
| Biological Process | GO:0007059 | chromosome segregation |
| Biological Process | GO:0006974 | DNA damage response |
| Biological Process | GO:0006281 | DNA repair |
| Biological Process | GO:0006351 | DNA-templated transcription |
| Biological Process | GO:0045738 | negative regulation of DNA repair |
| Biological Process | GO:0031397 | negative regulation of protein ubiquitination |
| Biological Process | GO:0017148 | negative regulation of translation |
| Biological Process | GO:2001235 | positive regulation of apoptotic signaling pathway |
| Biological Process | GO:1905053 | positive regulation of base-excision repair |
| Biological Process | GO:0045739 | positive regulation of DNA repair |
| Biological Process | GO:2000144 | positive regulation of DNA-templated transcription initiation |
| Biological Process | GO:0010628 | positive regulation of gene expression |
| Biological Process | GO:1902231 | positive regulation of intrinsic apoptotic signaling pathway in response to DNA damage |
| Biological Process | GO:0031116 | positive regulation of microtubule polymerization |
| Biological Process | GO:1901224 | positive regulation of non-canonical NF-kappaB signal transduction |
| Biological Process | GO:0042981 | regulation of apoptotic process |
| Biological Process | GO:0061481 | response to TNF agonist |
| Biological Process | GO:0051225 | spindle assembly |
| Biological Process | GO:0006412 | translation |
| Cellular Component | GO:0098556 | cytoplasmic side of rough endoplasmic reticulum membrane |
| Cellular Component | GO:0005829 | cytosol |
| Cellular Component | GO:0022626 | cytosolic ribosome |
| Cellular Component | GO:0022627 | cytosolic small ribosomal subunit |
| Cellular Component | GO:0030425 | dendrite |
| Cellular Component | GO:0005783 | endoplasmic reticulum |
| Cellular Component | GO:0005743 | mitochondrial inner membrane |
| Cellular Component | GO:0005759 | mitochondrial matrix |
| Cellular Component | GO:0072686 | mitotic spindle |
Reference
[1] Sheng L, Xu H, Wang Y, Ni J, Xiang T et al.. Systematic analysis of lysine lactylation in nucleus pulposus cells.. iScience 27(11):111157. 2024 Nov 15. PMID: 39524337.
[2] Chen Y, Sun W, Sun Z, Zhao H, Wu T et al.. Effect of electroacupuncture on hippocampal protein lactylation in a rat model of vascular dementia.. Front Neurol 16:1629474. 2025. PMID: 40963935.