Overview
| Uniprot ID | P62914 |
|---|
| Protein Name | Large ribosomal subunit protein uL5 |
|---|
| Gene Name | Rpl11 |
|---|
| Organism | Rattus norvegicus |
|---|
Kla Sites from experimental identification
| Position |
Flanking peptide |
| 52 |
GQTPVFSKARYTVRS |
Function
Component of the ribosome, a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell. The small ribosomal subunit (SSU) binds messenger RNAs (mRNAs) and translates the encoded message by selecting cognate aminoacyl-transfer RNA (tRNA) molecules. The large subunit (LSU) contains the ribosomal catalytic site termed the peptidyl transferase center (PTC), which catalyzes the formation of peptide bonds, thereby polymerizing the amino acids delivered by tRNAs into a polypeptide chain. The nascent polypeptides leave the ribosome through a tunnel in the LSU and interact with protein factors that function in enzymatic processing, targeting, and the membrane insertion of nascent chains at the exit of the ribosomal tunnel. As part of the 5S RNP/5S ribonucleoprotein particle it is an essential component of the LSU, required for its formation and the maturation of rRNAs. It also couples ribosome biogenesis to p53/TP53 activation. As part of the 5S RNP it accumulates in the nucleoplasm and inhibits MDM2, when ribosome biogenesis is perturbed, mediating the stabilization and the activation of TP53. Promotes nucleolar location of PML
Protein Sequence
10
MAQDQGEKEN
20
PMRELRIRKL
30
CLNICVGESG
40
DRLTRAAKVL
50
EQLTGQTPVF
60
SKARYTVRSF
70
GIRRNEKIAV
80
HCTVRGAKAE
90
EILEKGLKVR
100
EYELRKNNFS
110
DTGNFGFGIQ
120
EHIDLGIKYD
130
PSIGIYGLDF
140
YVVLGRPGFS
150
IADKKRRTGC
160
IGAKHRISKE
170
EAMRWFQQKY
DGIILPGK
Gene Ontology
| Classification |
GO ID |
Description |
| Biological Process |
GO:0032435 |
negative regulation of proteasomal ubiquitin-dependent protein catabolic process |
| Cellular Component |
GO:0005737 |
cytoplasm |
| Cellular Component |
GO:0098556 |
cytoplasmic side of rough endoplasmic reticulum membrane |
| Cellular Component |
GO:0022625 |
cytosolic large ribosomal subunit |
| Cellular Component |
GO:0022626 |
cytosolic ribosome |
| Cellular Component |
GO:0015934 |
large ribosomal subunit |
| Cellular Component |
GO:0005730 |
nucleolus |
| Cellular Component |
GO:0005654 |
nucleoplasm |
| Cellular Component |
GO:0098794 |
postsynapse |
| Cellular Component |
GO:0098793 |
presynapse |
| Cellular Component |
GO:0032991 |
protein-containing complex |
| Cellular Component |
GO:0005840 |
ribosome |
| Cellular Component |
GO:0045202 |
synapse |
| Molecular Function |
GO:0008097 |
5S rRNA binding |
| Molecular Function |
GO:0042975 |
peroxisome proliferator activated receptor binding |
| Molecular Function |
GO:0003723 |
RNA binding |
| Molecular Function |
GO:0003735 |
structural constituent of ribosome |
| Molecular Function |
GO:1990948 |
ubiquitin ligase inhibitor activity |
| Molecular Function |
GO:0031625 |
ubiquitin protein ligase binding |
| Biological Process |
GO:0002181 |
cytoplasmic translation |
| Biological Process |
GO:2000059 |
negative regulation of ubiquitin-dependent protein catabolic process |
| Biological Process |
GO:1901798 |
positive regulation of signal transduction by p53 class mediator |
| Biological Process |
GO:0034504 |
protein localization to nucleus |
| Biological Process |
GO:0006605 |
protein targeting |
| Biological Process |
GO:1901796 |
regulation of signal transduction by p53 class mediator |
| Biological Process |
GO:0000027 |
ribosomal large subunit assembly |
| Biological Process |
GO:0042273 |
ribosomal large subunit biogenesis |
| Biological Process |
GO:0006364 |
rRNA processing |
| Biological Process |
GO:0006412 |
translation |
| Biological Process |
GO:0140242 |
translation at postsynapse |
| Biological Process |
GO:0140236 |
translation at presynapse |
Reference
[1] Chen Y, Sun W, Sun Z, Zhao H, Wu T et al.. Effect of electroacupuncture on hippocampal protein lactylation in a rat model of vascular dementia.. Front Neurol 16:1629474. 2025. PMID: 40963935.