Search Results

Overview

Uniprot ID	P63036
Protein Name	DnaJ homolog subfamily A member 1
Gene Name	Dnaja1
Organism	Rattus norvegicus

Kla Sites from experimental identification

Position	Flanking peptide
32	AYRKLALKYHPDKNP
37	ALKYHPDKNPNEGEK

Function

Functions as a co-chaperone for HSPA1B and negatively regulates the translocation of BAX from the cytosol to mitochondria in response to cellular stress, thereby protecting cells against apoptosis. Promotes apoptosis in response to cellular stress mediated by exposure to anisomycin or UV. Stimulates ATP hydrolysis, but not the folding of unfolded proteins mediated by HSPA1A (in vitro) (By similarity). Co-chaperone for HSPA8/Hsc70. Plays a role in protein transport into mitochondria via its role as co-chaperone (PubMed:10816573)

Protein Sequence

10 MVKETTYYDV 20 LGVKPNATQE 30 ELKKAYRKLA 40 LKYHPDKNPN 50 EGEKFKQISQ 60 AYEVLADSKK 70 RELYDKGGEQ 80 AIKEGGAGGG 90 FGSPMDIFDM 100 FFGGGGRMQR 110 ERRGKNVVHQ 120 LSVTLEDLYN 130 GATRKLALQK 140 NVICDKCEGR 150 GGKKGAVECC 160 PNCRGTGMQI 170 RIHQIGPGMV 180 QQIQSVCMEC 190 QGHGERISPK 200 DRCKSCNGRK 210 IVREKKILEV 220 HIDKGMKDGQ 230 KITFHGEGDQ 240 EPGLEPGDII 250 IVLDQKDHAV 260 FTRRGEDLFM 270 CMDIQLVEAL 280 CGFQKPISTL 290 DNRTIVITSH 300 PGQIVKHGDI 310 KCVLNEGMPI 320 YRRPYEKGRL 330 IIEFKVNFPE 340 NGFLSPDKLS 350 LLEKLLPERK 360 EVEETDEMDQ 370 VELVDFDPNQ 380 ERRRHYNGEA 390 YEDDEHHPRG GVQCQTS

Gene Ontology

Classification	GO ID	Description
Cellular Component	GO:0005829	cytosol
Cellular Component	GO:0016020	membrane
Cellular Component	GO:0015630	microtubule cytoskeleton
Cellular Component	GO:0005739	mitochondrion
Cellular Component	GO:0005634	nucleus
Cellular Component	GO:0048471	perinuclear region of cytoplasm
Molecular Function	GO:0005524	ATP binding
Molecular Function	GO:0001671	ATPase activator activity
Molecular Function	GO:0055131	C3HC4-type RING finger domain binding
Molecular Function	GO:0001664	G protein-coupled receptor binding
Molecular Function	GO:0030544	Hsp70 protein binding
Molecular Function	GO:0050750	low-density lipoprotein particle receptor binding
Molecular Function	GO:0051087	protein-folding chaperone binding
Molecular Function	GO:0030957	Tat protein binding
Molecular Function	GO:0031625	ubiquitin protein ligase binding
Molecular Function	GO:0051082	unfolded protein binding
Molecular Function	GO:0008270	zinc ion binding
Biological Process	GO:0030521	androgen receptor signaling pathway
Biological Process	GO:0030317	flagellated sperm motility
Biological Process	GO:0043066	negative regulation of apoptotic process
Biological Process	GO:1903748	negative regulation of establishment of protein localization to mitochondrion
Biological Process	GO:0043508	negative regulation of JUN kinase activity
Biological Process	GO:1905259	negative regulation of nitrosative stress-induced intrinsic apoptotic signaling pathway
Biological Process	GO:0031397	negative regulation of protein ubiquitination
Biological Process	GO:0043065	positive regulation of apoptotic process
Biological Process	GO:0006457	protein folding
Biological Process	GO:0070585	protein localization to mitochondrion
Biological Process	GO:0051223	regulation of protein transport
Biological Process	GO:0009408	response to heat
Biological Process	GO:0007283	spermatogenesis

Reference

[1] Sheng L, Xu H, Wang Y, Ni J, Xiang T et al.. Systematic analysis of lysine lactylation in nucleus pulposus cells.. iScience 27(11):111157. 2024 Nov 15. PMID: 39524337.

[2] Chen Y, Sun W, Sun Z, Zhao H, Wu T et al.. Effect of electroacupuncture on hippocampal protein lactylation in a rat model of vascular dementia.. Front Neurol 16:1629474. 2025. PMID: 40963935.