Overview
| Uniprot ID | P63096 |
|---|
| Protein Name | Guanine nucleotide-binding protein G(i) subunit alpha-1 |
|---|
| Gene Name | GNAI1 |
|---|
| Organism | Homo sapiens |
|---|
Kla Sites from experimental identification
| Position |
Flanking peptide |
| 67 |
GYSEEECKQYKAVVY |
| 92 |
IRAMGRLKIDFGDSA |
Function
Guanine nucleotide-binding proteins (G proteins) function as transducers downstream of G protein-coupled receptors (GPCRs) in numerous signaling cascades (PubMed:18434541, PubMed:33762731, PubMed:34239069, PubMed:35610220, PubMed:37935376, PubMed:37935377, PubMed:37963465, PubMed:38552625, PubMed:8774883, PubMed:38918398, PubMed:40080544). The alpha chain contains the guanine nucleotide binding site and alternates between an active, GTP-bound state and an inactive, GDP-bound state (PubMed:18434541, PubMed:8774883). Signaling by an activated GPCR promotes GDP release and GTP binding (PubMed:18434541, PubMed:8774883). The alpha subunit has a low GTPase activity that converts bound GTP to GDP, thereby terminating the signal (PubMed:18434541, PubMed:8774883). Both GDP release and GTP hydrolysis are modulated by numerous regulatory proteins (PubMed:18434541, PubMed:8774883). Signaling is mediated via effector proteins, such as adenylate cyclase: inhibits adenylate cyclase activity of ADCY1, ADCY5 and ADCY6, leading to decreased intracellular cAMP levels (PubMed:8119955). The inactive GDP-bound form prevents the association of RGS14 with centrosomes and is required for the translocation of RGS14 from the cytoplasm to the plasma membrane. Required for normal cytokinesis during mitosis (PubMed:17635935). Required for cortical dynein-dynactin complex recruitment during metaphase (PubMed:22327364)
Protein Sequence
10
MGCTLSAEDK
20
AAVERSKMID
30
RNLREDGEKA
40
AREVKLLLLG
50
AGESGKSTIV
60
KQMKIIHEAG
70
YSEEECKQYK
80
AVVYSNTIQS
90
IIAIIRAMGR
100
LKIDFGDSAR
110
ADDARQLFVL
120
AGAAEEGFMT
130
AELAGVIKRL
140
WKDSGVQACF
150
NRSREYQLND
160
SAAYYLNDLD
170
RIAQPNYIPT
180
QQDVLRTRVK
190
TTGIVETHFT
200
FKDLHFKMFD
210
VGGQRSERKK
220
WIHCFEGVTA
230
IIFCVALSDY
240
DLVLAEDEEM
250
NRMHESMKLF
260
DSICNNKWFT
270
DTSIILFLNK
280
KDLFEEKIKK
290
SPLTICYPEY
300
AGSNTYEEAA
310
AYIQCQFEDL
320
NKRKDTKEIY
330
THFTCATDTK
340
NVQFVFDAVT
350
DVIIKNNLKD
CGLF
Gene Ontology
| Classification |
GO ID |
Description |
| Cellular Component |
GO:0005938 |
cell cortex |
| Cellular Component |
GO:0005813 |
centrosome |
| Cellular Component |
GO:0036064 |
ciliary basal body |
| Cellular Component |
GO:0005737 |
cytoplasm |
| Cellular Component |
GO:0005829 |
cytosol |
| Cellular Component |
GO:0070062 |
extracellular exosome |
| Cellular Component |
GO:0005834 |
heterotrimeric G-protein complex |
| Cellular Component |
GO:0005765 |
lysosomal membrane |
| Cellular Component |
GO:0030496 |
midbody |
| Cellular Component |
GO:0005634 |
nucleus |
| Cellular Component |
GO:0005886 |
plasma membrane |
| Molecular Function |
GO:0010855 |
adenylate cyclase inhibitor activity |
| Molecular Function |
GO:0031749 |
D2 dopamine receptor binding |
| Molecular Function |
GO:0003925 |
G protein activity |
| Molecular Function |
GO:0001664 |
G protein-coupled receptor binding |
| Molecular Function |
GO:0031821 |
G protein-coupled serotonin receptor binding |
| Molecular Function |
GO:0031683 |
G-protein beta/gamma-subunit complex binding |
| Molecular Function |
GO:0019003 |
GDP binding |
| Molecular Function |
GO:0005525 |
GTP binding |
| Molecular Function |
GO:0003924 |
GTPase activity |
| Molecular Function |
GO:0000287 |
magnesium ion binding |
| Biological Process |
GO:0007189 |
adenylate cyclase-activating G protein-coupled receptor signaling pathway |
| Biological Process |
GO:0007193 |
adenylate cyclase-inhibiting G protein-coupled receptor signaling pathway |
| Biological Process |
GO:0007198 |
adenylate cyclase-inhibiting serotonin receptor signaling pathway |
| Biological Process |
GO:0007188 |
adenylate cyclase-modulating G protein-coupled receptor signaling pathway |
| Biological Process |
GO:0051301 |
cell division |
| Biological Process |
GO:1904322 |
cellular response to forskolin |
| Biological Process |
GO:0070098 |
chemokine-mediated signaling pathway |
| Biological Process |
GO:0007186 |
G protein-coupled receptor signaling pathway |
| Biological Process |
GO:0043303 |
mast cell degranulation |
| Biological Process |
GO:0007218 |
neuropeptide signaling pathway |
| Biological Process |
GO:0045542 |
positive regulation of cholesterol biosynthetic process |
| Biological Process |
GO:1904778 |
positive regulation of protein localization to cell cortex |
| Biological Process |
GO:1901082 |
positive regulation of relaxation of smooth muscle |
| Biological Process |
GO:1903998 |
regulation of eating behavior |
| Biological Process |
GO:0060236 |
regulation of mitotic spindle organization |
| Biological Process |
GO:0043434 |
response to peptide hormone |
| Biological Process |
GO:0034695 |
response to prostaglandin E |
| Biological Process |
GO:0072678 |
T cell migration |
Reference
[1] Shi CM, Wang QC, Li XL, Yang YH, Tang XY et al.. Global Profiling of Protein Lactylation in Human Hippocampi.. Proteomics Clin Appl 19(2):e202400061. 2025 Mar. PMID: 39610256.