Overview
| Uniprot ID | P63209 |
|---|
| Protein Name | S-phase kinase-associated protein 1 |
|---|
| Gene Name | SKP1 |
|---|
| Organism | Cavia porcellus |
|---|
Kla Sites from experimental identification
| Position |
Flanking peptide |
| 155 |
EEEAQVRKENQWCEE |
Function
Essential component of the SCF (SKP1-CUL1-F-box protein) ubiquitin ligase complex, which mediates the ubiquitination of proteins involved in cell cycle progression, signal transduction and transcription. In the SCF complex, serves as an adapter that links the F-box protein to CUL1. The functional specificity of the SCF complex depends on the F-box protein as substrate recognition component. SCF(BTRC) and SCF(FBXW11) direct ubiquitination of CTNNB1 and participate in Wnt signaling. SCF(FBXW11) directs ubiquitination of phosphorylated NFKBIA. SCF(BTRC) directs ubiquitination of NFKBIB, NFKBIE, ATF4, SMAD3, SMAD4, CDC25A, FBXO5, CEP68 and probably NFKB2. SCF(SKP2) directs ubiquitination of phosphorylated CDKN1B/p27kip and is involved in regulation of G1/S transition. SCF(SKP2) directs ubiquitination of ORC1, CDT1, RBL2, ELF4, CDKN1A, RAG2, FOXO1A, and probably MYC and TAL1. SCF(FBXW7) directs ubiquitination of cyclin E, NOTCH1 released notch intracellular domain (NICD), and probably PSEN1. SCF(FBXW2) directs ubiquitination of GCM1. SCF(FBXO32) directs ubiquitination of MYOD1. SCF(FBXO7) directs ubiquitination of BIRC2 and DLGAP5. SCF(FBXO33) directs ubiquitination of YBX1. SCF(FBXO11) directs ubiquitination of BCL6 and DTL but does not seem to direct ubiquitination of TP53. SCF(BTRC) mediates the ubiquitination of NFKBIA at 'Lys-21' and 'Lys-22'; the degradation frees the associated NFKB1-RELA dimer to translocate into the nucleus and to activate transcription. SCF(CCNF) directs ubiquitination of CCP110. SCF(FBXL3) and SCF(FBXL21) direct ubiquitination of CRY1 and CRY2. SCF(FBXO9) direct ubiquitination of TTI1 and TELO2. SCF(FBXO10) direct ubiquitination of BCL2. Core component of the Cul7-RING(FBXW8) ubiquitin ligase complex, which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Also acts as a core component of the Cul1-RING(FBXL4) ubiquitin ligase complex, which mediates the ubiquitination and subsequent proteasomal degradation of BNIP3 and BNIP3L (By similarity)
Protein Sequence
10
MPSIKLQSSD
20
GEIFEVDVEI
30
AKQSVTIKTM
40
LEDLGMDDEG
50
DDDPVPLPNV
60
NAAILKKVIQ
70
WCTHHKDDPP
80
PPEDDENKEK
90
RTDDIPVWDQ
100
EFLKVDQGTL
110
FELILAANYL
120
DIKGLLDVTC
130
KTVANMIKGK
140
TPEEIRKTFN
150
IKNDFTEEEE
160
AQVRKENQWC
EEK
Gene Ontology
| Classification |
GO ID |
Description |
| Cellular Component |
GO:0031467 |
Cul7-RING ubiquitin ligase complex |
| Cellular Component |
GO:0005829 |
cytosol |
| Cellular Component |
GO:0019005 |
SCF ubiquitin ligase complex |
| Biological Process |
GO:0016567 |
protein ubiquitination |
| Biological Process |
GO:0031146 |
SCF-dependent proteasomal ubiquitin-dependent protein catabolic process |
Reference
[1] Feng J, Chen X, Li R, Xie Y, Zhang X et al.. Lactylome analysis reveals potential target modified proteins in the retina of form-deprivation myopia.. iScience 27(9):110606. 2024 Sep 20. PMID: 39246443.