Search Results

Overview

Uniprot IDP63246
Protein NameSmall ribosomal subunit protein RACK1
Gene NameRACK1
OrganismSus scrofa

Kla Sites from experimental identification

Position Flanking peptide
106 RRFVGHTKDVLSVAF
130 GSRDKTIKLWNTLGV
175 CGWDKLVKVWNLANC
183 VWNLANCKLKTNHIG
185 NLANCKLKTNHIGHT
38 ILSASRDKTIIMWKL

Function

Scaffolding protein involved in the recruitment, assembly and/or regulation of a variety of signaling molecules. Interacts with a wide variety of proteins and plays a role in many cellular processes. Component of the 40S ribosomal subunit involved in translational repression (By similarity). Involved in the initiation of the ribosome quality control (RQC), a pathway that takes place when a ribosome has stalled during translation, by promoting ubiquitination of a subset of 40S ribosomal subunits (By similarity). Binds to and stabilizes activated protein kinase C (PKC), increasing PKC-mediated phosphorylation. May recruit activated PKC to the ribosome, leading to phosphorylation of EIF6. Inhibits the activity of SRC kinases including SRC, LCK and YES1. Inhibits cell growth by prolonging the G0/G1 phase of the cell cycle. Enhances phosphorylation of BMAL1 by PRKCA and inhibits transcriptional activity of the BMAL1-CLOCK heterodimer. Facilitates ligand-independent nuclear translocation of AR following PKC activation, represses AR transactivation activity and is required for phosphorylation of AR by SRC. Modulates IGF1R-dependent integrin signaling and promotes cell spreading and contact with the extracellular matrix. Involved in PKC-dependent translocation of ADAM12 to the cell membrane. Promotes the ubiquitination and proteasome-mediated degradation of proteins such as CLEC1B and HIF1A. Required for VANGL2 membrane localization, inhibits Wnt signaling, and regulates cellular polarization and oriented cell division during gastrulation. Required for PTK2/FAK1 phosphorylation and dephosphorylation. Promotes apoptosis by increasing oligomerization of BAX and disrupting the interaction of BAX with the anti-apoptotic factor BCL2L. Inhibits TRPM6 channel activity. Regulates cell surface expression of some GPCRs such as TBXA2R. Plays a role in regulation of FLT1-mediated cell migration (By similarity). Regulates internalization of the muscarinic receptor CHRM2 (PubMed:20976005). Involved in the transport of ABCB4 from the Golgi to the apical bile canalicular membrane (By similarity). Acts as an adapter for the dephosphorylation and inactivation of AKT1 by promoting recruitment of PP2A phosphatase to AKT1 (By similarity)

Protein Sequence

10 MTEQMTLRGT 20 LKGHNGWVTQ 30 IATTPQFPDM 40 ILSASRDKTI 50 IMWKLTRDET 60 NYGIPQRALR 70 GHSHFVSDVV 80 ISSDGQFALS 90 GSWDGTLRLW 100 DLTTGTTTRR 110 FVGHTKDVLS 120 VAFSSDNRQI 130 VSGSRDKTIK 140 LWNTLGVCKY 150 TVQDESHSEW 160 VSCVRFSPNS 170 SNPIIVSCGW 180 DKLVKVWNLA 190 NCKLKTNHIG 200 HTGYLNTVTV 210 SPDGSLCASG 220 GKDGQAMLWD 230 LNEGKHLYTL 240 DGGDIINALC 250 FSPNRYWLCA 260 ATGPSIKIWD 270 LEGKIIVDEL 280 KQEVISTSSK 290 AEPPQCTSLA 300 WSADGQTLFA 310 GYTDNLVRVW QVTIGTR

Gene Ontology

Classification GO ID Description
Cellular Component GO:0005737 cytoplasm
Cellular Component GO:0005829 cytosol
Cellular Component GO:0030425 dendrite
Cellular Component GO:1990630 IRE1-RACK1-PP2A complex
Cellular Component GO:0030496 midbody
Cellular Component GO:0005739 mitochondrion
Cellular Component GO:0043025 neuronal cell body
Cellular Component GO:0005654 nucleoplasm
Cellular Component GO:0005634 nucleus
Cellular Component GO:0043204 perikaryon
Cellular Component GO:0048471 perinuclear region of cytoplasm
Cellular Component GO:0001891 phagocytic cup
Cellular Component GO:1990904 ribonucleoprotein complex
Cellular Component GO:0005840 ribosome
Molecular Function GO:0051434 BH3 domain binding
Molecular Function GO:0030332 cyclin binding
Molecular Function GO:0008656 cysteine-type endopeptidase activator activity involved in apoptotic process
Molecular Function GO:0042803 protein homodimerization activity
Molecular Function GO:0005080 protein kinase C binding
Molecular Function GO:0019903 protein phosphatase binding
Molecular Function GO:0030291 protein serine/threonine kinase inhibitor activity
Molecular Function GO:0030292 protein tyrosine kinase inhibitor activity
Molecular Function GO:0030971 receptor tyrosine kinase binding
Molecular Function GO:0043022 ribosome binding
Molecular Function GO:0042169 SH2 domain binding
Molecular Function GO:0035591 signaling adaptor activity
Molecular Function GO:0045182 translation regulator activity
Biological Process GO:0071333 cellular response to glucose stimulus
Biological Process GO:0071363 cellular response to growth factor stimulus
Biological Process GO:0007369 gastrulation
Biological Process GO:0030308 negative regulation of cell growth
Biological Process GO:1903751 negative regulation of intrinsic apoptotic signaling pathway in response to hydrogen peroxide
Biological Process GO:0050765 negative regulation of phagocytosis
Biological Process GO:0051898 negative regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction
Biological Process GO:0045879 negative regulation of smoothened signaling pathway
Biological Process GO:2001125 negative regulation of translational frameshifting
Biological Process GO:0030178 negative regulation of Wnt signaling pathway
Biological Process GO:0043065 positive regulation of apoptotic process
Biological Process GO:0030335 positive regulation of cell migration
Biological Process GO:2000543 positive regulation of gastrulation
Biological Process GO:0042998 positive regulation of Golgi to plasma membrane protein transport
Biological Process GO:0043547 positive regulation of GTPase activity
Biological Process GO:2001244 positive regulation of intrinsic apoptotic signaling pathway
Biological Process GO:0051901 positive regulation of mitochondrial depolarization
Biological Process GO:0032436 positive regulation of proteasomal ubiquitin-dependent protein catabolic process
Biological Process GO:0001934 positive regulation of protein phosphorylation
Biological Process GO:0031334 positive regulation of protein-containing complex assembly
Biological Process GO:0016567 protein ubiquitination
Biological Process GO:0106070 regulation of adenylate cyclase-activating G protein-coupled receptor signaling pathway
Biological Process GO:0051726 regulation of cell cycle
Biological Process GO:0051302 regulation of cell division
Biological Process GO:2000114 regulation of establishment of cell polarity
Biological Process GO:0032880 regulation of protein localization
Biological Process GO:0072344 rescue of stalled cytosolic ribosome
Biological Process GO:0048511 rhythmic process

Reference

[1] Fan S, Zhou R, Wen H, Ye H, Ma S et al.. Global profiling of protein lactylome in porcine granulosa cells.. J Ovarian Res 18(1):177. 2025 Aug 8. PMID: 40781717.