Search Results
Overview
| Uniprot ID | P67809 |
|---|---|
| Protein Name | Y-box-binding protein 1 |
| Gene Name | YBX1 |
| Organism | Homo sapiens |
Kla Sites from experimental identification
| Position | Flanking peptide |
|---|---|
| 137 | GVPVQGSKYAADRNH |
| 301 | NPKPQDGKETKAADP |
| 81 | FINRNDTKEDVFVHQ |
| 92 | FVHQTAIKKNNPRKY |
| 93 | VHQTAIKKNNPRKYL |
Function
DNA- and RNA-binding protein involved in various processes, such as translational repression, RNA stabilization, mRNA splicing, DNA repair and transcription regulation (PubMed:10817758, PubMed:11698476, PubMed:14718551, PubMed:18809583, PubMed:31358969, PubMed:8188694). Predominantly acts as a RNA-binding protein: binds preferentially to the 5'-[CU]CUGCG-3' RNA motif and specifically recognizes mRNA transcripts modified by C5-methylcytosine (m5C) (PubMed:19561594, PubMed:31358969). Promotes mRNA stabilization: acts by binding to m5C-containing mRNAs and recruiting the mRNA stability maintainer ELAVL1, thereby preventing mRNA decay (PubMed:10817758, PubMed:11698476, PubMed:31358969). Component of the CRD-mediated complex that promotes MYC mRNA stability (PubMed:19029303). Contributes to the regulation of translation by modulating the interaction between the mRNA and eukaryotic initiation factors (By similarity). Plays a key role in RNA composition of extracellular exosomes by defining the sorting of small non-coding RNAs, such as tRNAs, Y RNAs, Vault RNAs and miRNAs (PubMed:27559612, PubMed:29073095). Probably sorts RNAs in exosomes by recognizing and binding C5-methylcytosine (m5C)-containing RNAs (PubMed:28341602, PubMed:29073095). Acts as a key effector of epidermal progenitors by preventing epidermal progenitor senescence: acts by regulating the translation of a senescence-associated subset of cytokine mRNAs, possibly by binding to m5C-containing mRNAs (PubMed:29712925). Also involved in pre-mRNA alternative splicing regulation: binds to splice sites in pre-mRNA and regulates splice site selection (PubMed:12604611). Binds to TSC22D1 transcripts, thereby inhibiting their translation and negatively regulating TGF-beta-mediated transcription of COL1A2 (By similarity). Also able to bind DNA: regulates transcription of the multidrug resistance gene MDR1 is enhanced in presence of the APEX1 acetylated form at 'Lys-6' and 'Lys-7' (PubMed:18809583). Binds to promoters that contain a Y-box (5'-CTGATTGGCCAA-3'), such as MDR1 and HLA class II genes (PubMed:18809583, PubMed:8188694). Promotes separation of DNA strands that contain mismatches or are modified by cisplatin (PubMed:14718551). Has endonucleolytic activity and can introduce nicks or breaks into double-stranded DNA, suggesting a role in DNA repair (PubMed:14718551). The secreted form acts as an extracellular mitogen and stimulates cell migration and proliferation (PubMed:19483673)
Protein Sequence
Gene Ontology
| Classification | GO ID | Description |
|---|---|---|
| Cellular Component | GO:0070937 | CRD-mediated mRNA stability complex |
| Cellular Component | GO:0005737 | cytoplasm |
| Cellular Component | GO:0010494 | cytoplasmic stress granule |
| Cellular Component | GO:0005829 | cytosol |
| Cellular Component | GO:0070062 | extracellular exosome |
| Cellular Component | GO:0005576 | extracellular region |
| Cellular Component | GO:0071204 | histone pre-mRNA 3'end processing complex |
| Cellular Component | GO:0005654 | nucleoplasm |
| Cellular Component | GO:0005634 | nucleus |
| Cellular Component | GO:0000932 | P-body |
| Cellular Component | GO:1990904 | ribonucleoprotein complex |
| Cellular Component | GO:0045202 | synapse |
| Cellular Component | GO:0005689 | U12-type spliceosomal complex |
| Molecular Function | GO:0062153 | C5-methylcytidine-containing RNA reader activity |
| Molecular Function | GO:0003682 | chromatin binding |
| Molecular Function | GO:0003677 | DNA binding |
| Molecular Function | GO:0003690 | double-stranded DNA binding |
| Molecular Function | GO:0051020 | GTPase binding |
| Molecular Function | GO:0035198 | miRNA binding |
| Molecular Function | GO:0003729 | mRNA binding |
| Molecular Function | GO:0003676 | nucleic acid binding |
| Molecular Function | GO:0003723 | RNA binding |
| Molecular Function | GO:1990837 | sequence-specific double-stranded DNA binding |
| Molecular Function | GO:0003697 | single-stranded DNA binding |
| Biological Process | GO:0070934 | CRD-mediated mRNA stabilization |
| Biological Process | GO:0006351 | DNA-templated transcription |
| Biological Process | GO:0048598 | embryonic morphogenesis |
| Biological Process | GO:0008544 | epidermis development |
| Biological Process | GO:1990428 | miRNA transport |
| Biological Process | GO:0006397 | mRNA processing |
| Biological Process | GO:0048255 | mRNA stabilization |
| Biological Process | GO:2000773 | negative regulation of cellular senescence |
| Biological Process | GO:1900152 | negative regulation of nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay |
| Biological Process | GO:0017148 | negative regulation of translation |
| Biological Process | GO:0051781 | positive regulation of cell division |
| Biological Process | GO:2000767 | positive regulation of cytoplasmic translation |
| Biological Process | GO:0045944 | positive regulation of transcription by RNA polymerase II |
| Biological Process | GO:1903608 | protein localization to cytoplasmic stress granule |
| Biological Process | GO:0006355 | regulation of DNA-templated transcription |
| Biological Process | GO:0010468 | regulation of gene expression |
| Biological Process | GO:0008380 | RNA splicing |
| Biological Process | GO:0050658 | RNA transport |
| Biological Process | GO:0051031 | tRNA transport |
Reference
[1] Yang Z, Yan C, Ma J, Peng P, Ren X et al.. Lactylome analysis suggests lactylation-dependent mechanisms of metabolic adaptation in hepatocellular carcinoma.. Nat Metab 5(1):61-79. 2023 Jan. PMID: 36593272.
[2] Hong H, Chen X, Wang H, Gu X, Yuan Y et al.. Global profiling of protein lysine lactylation and potential target modified protein analysis in hepatocellular carcinoma.. Proteomics 23(9):e2200432. 2023 May. PMID: 36625413.
[3] Wu Q, Li Z, Gong T, Zheng X, Zhou X et al.. Porphyromonas gingivalis infection induces lysine lactylation reprogramming in human umbilical vein endothelial cells.. Front Cell Infect Microbiol 16:1706727. 2026. PMID: 41696360.