Search Results
Overview
| Uniprot ID | P68403 |
|---|---|
| Protein Name | Protein kinase C beta type |
| Gene Name | Prkcb |
| Organism | Rattus norvegicus |
Kla Sites from experimental identification
| Position | Flanking peptide |
|---|---|
| 205 | LKLIPDPKSESKQKT |
| 216 | KQKTKTIKCSLNPEW |
| 315 | AKIGQGTKAPEEKTA |
| 327 | KTANTISKFDNNGNR |
| 38 | VHEVKNHKFTARFFK |
| 566 | AYPKSMSKEAVAICK |
| 573 | KEAVAICKGLMTKHP |
Function
Calcium-activated, phospholipid- and diacylglycerol (DAG)-dependent serine/threonine-protein kinase involved in various cellular processes such as regulation of the B-cell receptor (BCR) signalosome, oxidative stress-induced apoptosis, androgen receptor-dependent transcription regulation, insulin signaling and endothelial cells proliferation. Plays a key role in B-cell activation by regulating BCR-induced NF-kappa-B activation. Mediates the activation of the canonical NF-kappa-B pathway (NFKB1) by direct phosphorylation of CARD11/CARMA1 at 'Ser-559', 'Ser-644' and 'Ser-652'. Phosphorylation induces CARD11/CARMA1 association with lipid rafts and recruitment of the BCL10-MALT1 complex as well as MAP3K7/TAK1, which then activates IKK complex, resulting in nuclear translocation and activation of NFKB1. Plays a direct role in the negative feedback regulation of the BCR signaling, by down-modulating BTK function via direct phosphorylation of BTK at 'Ser-180', which results in the alteration of BTK plasma membrane localization and in turn inhibition of BTK activity. Involved in apoptosis following oxidative damage: in case of oxidative conditions, specifically phosphorylates 'Ser-36' of isoform p66Shc of SHC1, leading to mitochondrial accumulation of p66Shc, where p66Shc acts as a reactive oxygen species producer. Acts as a coactivator of androgen receptor (ANDR)-dependent transcription, by being recruited to ANDR target genes and specifically mediating phosphorylation of 'Thr-6' of histone H3 (H3T6ph), a specific tag for epigenetic transcriptional activation that prevents demethylation of histone H3 'Lys-4' (H3K4me) by LSD1/KDM1A. In insulin signaling, may function downstream of IRS1 in muscle cells and mediate insulin-dependent DNA synthesis through the RAF1-MAPK/ERK signaling cascade. Participates in the regulation of glucose transport in adipocytes by negatively modulating the insulin-stimulated translocation of the glucose transporter SLC2A4/GLUT4. Phosphorylates SLC2A1/GLUT1, promoting glucose uptake by SLC2A1/GLUT1. Under high glucose in pancreatic beta-cells, is probably involved in the inhibition of the insulin gene transcription, via regulation of MYC expression. In endothelial cells, activation of PRKCB induces increased phosphorylation of RB1, increased VEGFA-induced cell proliferation, and inhibits PI3K/AKT-dependent nitric oxide synthase (NOS3/eNOS) regulation by insulin, which causes endothelial dysfunction. Also involved in triglyceride homeostasis (By similarity). Phosphorylates ATF2 which promotes cooperation between ATF2 and JUN, activating transcription. Phosphorylates KLHL3 in response to angiotensin II signaling, decreasing the interaction between KLHL3 and WNK4 (By similarity). Phosphorylates and activates LRRK1, which phosphorylates RAB proteins involved in intracellular trafficking (By similarity)
Protein Sequence
Gene Ontology
| Classification | GO ID | Description |
|---|---|---|
| Cellular Component | GO:0031526 | brush border membrane |
| Biological Process | GO:0030949 | positive regulation of vascular endothelial growth factor receptor signaling pathway |
| Biological Process | GO:0070528 | protein kinase C signaling |
| Biological Process | GO:0006468 | protein phosphorylation |
| Biological Process | GO:0010827 | regulation of D-glucose transmembrane transport |
| Biological Process | GO:0014059 | regulation of dopamine secretion |
| Biological Process | GO:0040008 | regulation of growth |
| Biological Process | GO:2000300 | regulation of synaptic vesicle exocytosis |
| Biological Process | GO:0006357 | regulation of transcription by RNA polymerase II |
| Biological Process | GO:1990776 | response to angiotensin |
| Biological Process | GO:0045471 | response to ethanol |
| Biological Process | GO:0009749 | response to glucose |
| Biological Process | GO:0001666 | response to hypoxia |
| Biological Process | GO:0033280 | response to vitamin D |
| Biological Process | GO:0009410 | response to xenobiotic stimulus |
| Cellular Component | GO:0044305 | calyx of Held |
| Cellular Component | GO:0005813 | centrosome |
| Cellular Component | GO:0005737 | cytoplasm |
| Cellular Component | GO:0005829 | cytosol |
| Cellular Component | GO:0016020 | membrane |
| Cellular Component | GO:0005654 | nucleoplasm |
| Cellular Component | GO:0005634 | nucleus |
| Cellular Component | GO:0005886 | plasma membrane |
| Cellular Component | GO:0099523 | presynaptic cytosol |
| Cellular Component | GO:0008091 | spectrin |
| Molecular Function | GO:0005524 | ATP binding |
| Molecular Function | GO:0005246 | calcium channel regulator activity |
| Molecular Function | GO:0004698 | calcium,diacylglycerol-dependent serine/threonine kinase activity |
| Molecular Function | GO:0003682 | chromatin binding |
| Molecular Function | GO:0004697 | diacylglycerol-dependent serine/threonine kinase activity |
| Molecular Function | GO:0042393 | histone binding |
| Molecular Function | GO:0035403 | histone H3T6 kinase activity |
| Molecular Function | GO:0050681 | nuclear androgen receptor binding |
| Molecular Function | GO:0005080 | protein kinase C binding |
| Molecular Function | GO:0106310 | protein serine kinase activity |
| Molecular Function | GO:0004674 | protein serine/threonine kinase activity |
| Molecular Function | GO:0003713 | transcription coactivator activity |
| Molecular Function | GO:0008270 | zinc ion binding |
| Biological Process | GO:0002250 | adaptive immune response |
| Biological Process | GO:0006915 | apoptotic process |
| Biological Process | GO:0042113 | B cell activation |
| Biological Process | GO:0050853 | B cell receptor signaling pathway |
| Biological Process | GO:0006816 | calcium ion transport |
| Biological Process | GO:0071322 | cellular response to carbohydrate stimulus |
| Biological Process | GO:0018894 | dibenzo-p-dioxin metabolic process |
| Biological Process | GO:0006351 | DNA-templated transcription |
| Biological Process | GO:0006874 | intracellular calcium ion homeostasis |
| Biological Process | GO:0035556 | intracellular signal transduction |
| Biological Process | GO:0010829 | negative regulation of D-glucose transmembrane transport |
| Biological Process | GO:0046627 | negative regulation of insulin receptor signaling pathway |
| Biological Process | GO:0007207 | phospholipase C-activating G protein-coupled acetylcholine receptor signaling pathway |
| Biological Process | GO:0045766 | positive regulation of angiogenesis |
| Biological Process | GO:0043123 | positive regulation of canonical NF-kappaB signal transduction |
| Biological Process | GO:0160020 | positive regulation of ferroptosis |
| Biological Process | GO:0042488 | positive regulation of odontogenesis of dentin-containing tooth |
Reference
[1] Yao Y, Bade R, Li G, Zhang A, Zhao H et al.. Global-Scale Profiling of Differential Expressed Lysine-Lactylated Proteins in the Cerebral Endothelium of Cerebral Ischemia-Reperfusion Injury Rats.. Cell Mol Neurobiol 43(5):1989-2004. 2023 Jul. PMID: 36030297.
[2] Chen Y, Sun W, Sun Z, Zhao H, Wu T et al.. Effect of electroacupuncture on hippocampal protein lactylation in a rat model of vascular dementia.. Front Neurol 16:1629474. 2025. PMID: 40963935.