Overview
| Uniprot ID | P70349 |
|---|
| Protein Name | Adenosine 5'-monophosphoramidase HINT1 |
|---|
| Gene Name | Hint1 |
|---|
| Organism | Mus musculus |
|---|
Kla Sites from experimental identification
| Position |
Flanking peptide |
| 91 |
CAADLGLKRGYRMVV |
Function
Exhibits adenosine 5'-monophosphoramidase activity, hydrolyzing purine nucleotide phosphoramidates with a single phosphate group such as adenosine 5'monophosphoramidate (AMP-NH2) to yield AMP and NH2 (By similarity). Hydrolyzes adenosine 5'monophosphomorpholidate (AMP-morpholidate) and guanosine 5'monophosphomorpholidate (GMP-morpholidate) (By similarity). Hydrolyzes lysyl-AMP (AMP-N-epsilon-(N-alpha-acetyl lysine methyl ester)) generated by lysine tRNA ligase, as well as Met-AMP, His-AMP and Asp-AMP, lysyl-GMP (GMP-N-epsilon-(N-alpha-acetyl lysine methyl ester)) and AMP-N-alanine methyl ester (By similarity). Can also convert adenosine 5'-O-phosphorothioate and guanosine 5'-O-phosphorothioate to the corresponding nucleoside 5'-O-phosphates with concomitant release of hydrogen sulfide (By similarity). In addition, functions as a scaffolding protein that modulates transcriptional activation by the LEF1/TCF1-CTNNB1 complex and by the complex formed with MITF and CTNNB1 (By similarity). Modulates p53/TP53 levels and p53/TP53-mediated apoptosis (PubMed:35605301). Modulates proteasomal degradation of target proteins by the SCF (SKP2-CUL1-F-box protein) E3 ubiquitin-protein ligase complex (By similarity). Also exhibits SUMO-specific isopeptidase activity, deconjugating SUMO1 from RANGAP1 and RGS17 (PubMed:31088288)
Protein Sequence
10
MADEIAKAQV
20
AQPGGDTIFG
30
KIIRKEIPAK
40
IIFEDDRCLA
50
FHDISPQAPT
60
HFLVIPKKHI
70
SQISVADDDD
80
ESLLGHLMIV
90
GKKCAADLGL
100
KRGYRMVVNE
110
GADGGQSVYH
120
IHLHVLGGRQ
MNWPPG
Gene Ontology
| Classification |
GO ID |
Description |
| Cellular Component |
GO:0005829 |
cytosol |
| Cellular Component |
GO:0000118 |
histone deacetylase complex |
| Cellular Component |
GO:0005654 |
nucleoplasm |
| Cellular Component |
GO:0005634 |
nucleus |
| Cellular Component |
GO:0005886 |
plasma membrane |
| Molecular Function |
GO:0043530 |
adenosine 5'-monophosphoramidase activity |
| Molecular Function |
GO:0016929 |
deSUMOylase activity |
| Molecular Function |
GO:0016787 |
hydrolase activity |
| Molecular Function |
GO:0000166 |
nucleotide binding |
| Biological Process |
GO:0006351 |
DNA-templated transcription |
| Biological Process |
GO:0072332 |
intrinsic apoptotic signaling pathway by p53 class mediator |
| Biological Process |
GO:0050850 |
positive regulation of calcium-mediated signaling |
| Biological Process |
GO:0016926 |
protein desumoylation |
| Biological Process |
GO:0006508 |
proteolysis |
| Biological Process |
GO:0009154 |
purine ribonucleotide catabolic process |
| Biological Process |
GO:0006355 |
regulation of DNA-templated transcription |
| Cellular Component |
GO:0005737 |
cytoplasm |
Reference
[1] Wu D, Tang Y, Li X, Xiong S, Zhang Z et al.. Characterization of protein lactylation in healthy and ischemic mouse hearts.. Front Cardiovasc Med 12:1644886. 2025. PMID: 41089239.