Search Results

Overview

Uniprot IDP78324
Protein NameTyrosine-protein phosphatase non-receptor type substrate 1
Gene NameSIRPA
OrganismHomo sapiens

Kla Sites from experimental identification

Position Flanking peptide
483 VHLNRTPKQPAPKPE

Function

Immunoglobulin-like cell surface receptor for CD47. Acts as docking protein and induces translocation of PTPN6, PTPN11 and other binding partners from the cytosol to the plasma membrane. Supports adhesion of cerebellar neurons, neurite outgrowth and glial cell attachment. May play a key role in intracellular signaling during synaptogenesis and in synaptic function (By similarity). Involved in the negative regulation of receptor tyrosine kinase-coupled cellular responses induced by cell adhesion, growth factors or insulin. Mediates negative regulation of phagocytosis, mast cell activation and dendritic cell activation. CD47 binding prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. Plays a role in antiviral immunity and limits new world arenavirus infection by decreasing virus internalization (By similarity). Receptor for THBS1 (PubMed:24511121). Interaction with THBS1 stimulates phosphorylation of SIRPA (By similarity). In response to THBS1, involved in ROS signaling in non-phagocytic cells, stimulating NADPH oxidase-derived ROS production (PubMed:24511121)

Protein Sequence

10 MEPAGPAPGR 20 LGPLLCLLLA 30 ASCAWSGVAG 40 EEELQVIQPD 50 KSVLVAAGET 60 ATLRCTATSL 70 IPVGPIQWFR 80 GAGPGRELIY 90 NQKEGHFPRV 100 TTVSDLTKRN 110 NMDFSIRIGN 120 ITPADAGTYY 130 CVKFRKGSPD 140 DVEFKSGAGT 150 ELSVRAKPSA 160 PVVSGPAARA 170 TPQHTVSFTC 180 ESHGFSPRDI 190 TLKWFKNGNE 200 LSDFQTNVDP 210 VGESVSYSIH 220 STAKVVLTRE 230 DVHSQVICEV 240 AHVTLQGDPL 250 RGTANLSETI 260 RVPPTLEVTQ 270 QPVRAENQVN 280 VTCQVRKFYP 290 QRLQLTWLEN 300 GNVSRTETAS 310 TVTENKDGTY 320 NWMSWLLVNV 330 SAHRDDVKLT 340 CQVEHDGQPA 350 VSKSHDLKVS 360 AHPKEQGSNT 370 AAENTGSNER 380 NIYIVVGVVC 390 TLLVALLMAA 400 LYLVRIRQKK 410 AQGSTSSTRL 420 HEPEKNAREI 430 TQDTNDITYA 440 DLNLPKGKKP 450 APQAAEPNNH 460 TEYASIQTSP 470 QPASEDTLTY 480 ADLDMVHLNR 490 TPKQPAPKPE 500 PSFSEYASVQ VPRK

Gene Ontology

Classification GO ID Description
Cellular Component GO:0009986 cell surface
Cellular Component GO:0070062 extracellular exosome
Cellular Component GO:0101003 ficolin-1-rich granule membrane
Cellular Component GO:0016020 membrane
Cellular Component GO:0005886 plasma membrane
Cellular Component GO:0070821 tertiary granule membrane
Molecular Function GO:0098632 cell-cell adhesion mediator activity
Molecular Function GO:0030695 GTPase regulator activity
Molecular Function GO:1990405 protein antigen binding
Molecular Function GO:0086080 protein binding involved in heterotypic cell-cell adhesion
Molecular Function GO:0019903 protein phosphatase binding
Molecular Function GO:0004864 protein phosphatase inhibitor activity
Molecular Function GO:1990782 protein tyrosine kinase binding
Molecular Function GO:0017124 SH3 domain binding
Biological Process GO:0007155 cell adhesion
Biological Process GO:0016477 cell migration
Biological Process GO:0070301 cellular response to hydrogen peroxide
Biological Process GO:0071347 cellular response to interleukin-1
Biological Process GO:0071349 cellular response to interleukin-12
Biological Process GO:0071346 cellular response to type II interferon
Biological Process GO:0035696 monocyte extravasation
Biological Process GO:0043124 negative regulation of canonical NF-kappaB signal transduction
Biological Process GO:0071650 negative regulation of chemokine (C-C motif) ligand 5 production
Biological Process GO:1900016 negative regulation of cytokine production involved in inflammatory response
Biological Process GO:0070373 negative regulation of ERK1 and ERK2 cascade
Biological Process GO:0050728 negative regulation of inflammatory response
Biological Process GO:0032688 negative regulation of interferon-beta production
Biological Process GO:0032715 negative regulation of interleukin-6 production
Biological Process GO:0046329 negative regulation of JNK cascade
Biological Process GO:0031665 negative regulation of lipopolysaccharide-mediated signaling pathway
Biological Process GO:0071641 negative regulation of macrophage inflammatory protein 1 alpha production
Biological Process GO:0045019 negative regulation of nitric oxide biosynthetic process
Biological Process GO:0050765 negative regulation of phagocytosis
Biological Process GO:0032720 negative regulation of tumor necrosis factor production
Biological Process GO:0050766 positive regulation of phagocytosis
Biological Process GO:2000379 positive regulation of reactive oxygen species metabolic process
Biological Process GO:0050870 positive regulation of T cell activation
Biological Process GO:0010468 regulation of gene expression
Biological Process GO:0032651 regulation of interleukin-1 beta production
Biological Process GO:0032675 regulation of interleukin-6 production
Biological Process GO:0045428 regulation of nitric oxide biosynthetic process
Biological Process GO:0032680 regulation of tumor necrosis factor production
Biological Process GO:0032649 regulation of type II interferon production

Reference

[1] Shi CM, Wang QC, Li XL, Yang YH, Tang XY et al.. Global Profiling of Protein Lactylation in Human Hippocampi.. Proteomics Clin Appl 19(2):e202400061. 2025 Mar. PMID: 39610256.