Overview
| Uniprot ID | P78356 |
|---|
| Protein Name | Phosphatidylinositol 5-phosphate 4-kinase type-2 beta |
|---|
| Gene Name | PIP4K2B |
|---|
| Organism | Homo sapiens |
|---|
Kla Sites from experimental identification
| Position |
Flanking peptide |
| 150 |
YDRRFVIKTVSSEDV |
| 233 |
ASDKEKAKDLPTFKD |
Function
Participates in the biosynthesis of phosphatidylinositol 4,5-bisphosphate (PubMed:26774281, PubMed:9038203). Preferentially utilizes GTP, rather than ATP, for PI(5)P phosphorylation and its activity reflects changes in direct proportion to the physiological GTP concentration (PubMed:26774281). Its GTP-sensing activity is critical for metabolic adaptation (PubMed:26774281). PIP4Ks negatively regulate insulin signaling through a catalytic-independent mechanism. They interact with PIP5Ks and suppress PIP5K-mediated PtdIns(4,5)P2 synthesis and insulin-dependent conversion to PtdIns(3,4,5)P3 (PubMed:31091439)
Protein Sequence
10
MSSNCTSTTA
20
VAVAPLSASK
30
TKTKKKHFVC
40
QKVKLFRASE
50
PILSVLMWGV
60
NHTINELSNV
70
PVPVMLMPDD
80
FKAYSKIKVD
90
NHLFNKENLP
100
SRFKFKEYCP
110
MVFRNLRERF
120
GIDDQDYQNS
130
VTRSAPINSD
140
SQGRCGTRFL
150
TTYDRRFVIK
160
TVSSEDVAEM
170
HNILKKYHQF
180
IVECHGNTLL
190
PQFLGMYRLT
200
VDGVETYMVV
210
TRNVFSHRLT
220
VHRKYDLKGS
230
TVAREASDKE
240
KAKDLPTFKD
250
NDFLNEGQKL
260
HVGEESKKNF
270
LEKLKRDVEF
280
LAQLKIMDYS
290
LLVGIHDVDR
300
AEQEEMEVEE
310
RAEDEECEND
320
GVGGNLLCSY
330
GTPPDSPGNL
340
LSFPRFFGPG
350
EFDPSVDVYA
360
MKSHESSPKK
370
EVYFMAIIDI
380
LTPYDTKKKA
390
AHAAKTVKHG
400
AGAEISTVNP
410
EQYSKRFNEF
MSNILT
Gene Ontology
| Classification |
GO ID |
Description |
| Cellular Component |
GO:0005776 |
autophagosome |
| Cellular Component |
GO:0005829 |
cytosol |
| Cellular Component |
GO:0005789 |
endoplasmic reticulum membrane |
| Cellular Component |
GO:0005654 |
nucleoplasm |
| Cellular Component |
GO:0005634 |
nucleus |
| Cellular Component |
GO:0005886 |
plasma membrane |
| Molecular Function |
GO:0016308 |
1-phosphatidylinositol-4-phosphate 5-kinase activity |
| Molecular Function |
GO:0016309 |
1-phosphatidylinositol-5-phosphate 4-kinase activity |
| Molecular Function |
GO:0005524 |
ATP binding |
| Molecular Function |
GO:0005525 |
GTP binding |
| Molecular Function |
GO:0042803 |
protein homodimerization activity |
| Biological Process |
GO:1902635 |
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate biosynthetic process |
| Biological Process |
GO:0061909 |
autophagosome-lysosome fusion |
| Biological Process |
GO:0007166 |
cell surface receptor signaling pathway |
| Biological Process |
GO:0046627 |
negative regulation of insulin receptor signaling pathway |
| Biological Process |
GO:0046854 |
phosphatidylinositol phosphate biosynthetic process |
| Biological Process |
GO:2000786 |
positive regulation of autophagosome assembly |
| Biological Process |
GO:0010506 |
regulation of autophagy |
Reference
[1] Shi CM, Wang QC, Li XL, Yang YH, Tang XY et al.. Global Profiling of Protein Lactylation in Human Hippocampi.. Proteomics Clin Appl 19(2):e202400061. 2025 Mar. PMID: 39610256.