Overview
| Uniprot ID | P84092 |
|---|
| Protein Name | AP-2 complex subunit mu |
|---|
| Gene Name | Ap2m1 |
|---|
| Organism | Rattus norvegicus |
|---|
Kla Sites from experimental identification
| Position |
Flanking peptide |
| 224 |
NDKIVIEKQGKGTAD |
| 256 |
HQCVRLSKFDSERSI |
| 312 |
VVIKSNFKPSLLAQK |
| 345 |
MKGKAKYKASENAIV |
| 405 |
GLKVRYLKVFEPKLN |
| 410 |
YLKVFEPKLNYSDHD |
| 59 |
RTSFFHVKRSNIWLA |
Function
Component of the adaptor protein complex 2 (AP-2) (PubMed:14745134, PubMed:15473838). Adaptor protein complexes function in protein transport via transport vesicles in different membrane traffic pathways (PubMed:14745134, PubMed:15473838). Adaptor protein complexes are vesicle coat components and appear to be involved in cargo selection and vesicle formation (PubMed:14745134, PubMed:15473838). AP-2 is involved in clathrin-dependent endocytosis in which cargo proteins are incorporated into vesicles surrounded by clathrin (clathrin-coated vesicles, CCVs) which are destined for fusion with the early endosome (PubMed:14745134, PubMed:15473838). The clathrin lattice serves as a mechanical scaffold but is itself unable to bind directly to membrane components (PubMed:14745134, PubMed:15473838). Clathrin-associated adaptor protein (AP) complexes which can bind directly to both the clathrin lattice and to the lipid and protein components of membranes are considered to be the major clathrin adaptors contributing the CCV formation (PubMed:14745134, PubMed:15473838). AP-2 also serves as a cargo receptor to selectively sort the membrane proteins involved in receptor-mediated endocytosis (PubMed:14745134, PubMed:15473838). AP-2 seems to play a role in the recycling of synaptic vesicle membranes from the presynaptic surface (By similarity). AP-2 recognizes Y-X-X-[FILMV] (Y-X-X-Phi) and [ED]-X-X-X-L-[LI] endocytosis signal motifs within the cytosolic tails of transmembrane cargo molecules (PubMed:15985462). AP-2 may also play a role in maintaining normal post-endocytic trafficking through the ARF6-regulated, non-clathrin pathway (By similarity). During long-term potentiation in hippocampal neurons, AP-2 is responsible for the endocytosis of ADAM10 (By similarity). The AP-2 mu (AP2M1) subunit binds to transmembrane cargo proteins; it recognizes the Y-X-X-Phi motifs (PubMed:15985462). The surface region interacting with to the Y-X-X-Phi motif is inaccessible in cytosolic AP-2, but becomes accessible through a conformational change following phosphorylation of AP-2 mu subunit at Thr-156 in membrane-associated AP-2 (PubMed:11516654, PubMed:15985462). The membrane-specific phosphorylation event appears to involve assembled clathrin which activates the AP-2 mu kinase AAK1 (By similarity). Plays a role in endocytosis of frizzled family members upon Wnt signaling (PubMed:20947020)
Protein Sequence
10
MIGGLFIYNH
20
KGEVLISRVY
30
RDDIGRNAVD
40
AFRVNVIHAR
50
QQVRSPVTNI
60
ARTSFFHVKR
70
SNIWLAAVTK
80
QNVNAAMVFE
90
FLYKMCDVMA
100
AYFGKISEEN
110
IKNNFVLIYE
120
LLDEILDFGY
130
PQNSETGALK
140
TFITQQGIKS
150
QHQTKEEQSQ
160
ITSQVTGQIG
170
WRREGIKYRR
180
NELFLDVLES
190
VNLLMSPQGQ
200
VLSAHVSGRV
210
VMKSYLSGMP
220
ECKFGMNDKI
230
VIEKQGKGTA
240
DETSKSGKQS
250
IAIDDCTFHQ
260
CVRLSKFDSE
270
RSISFIPPDG
280
EFELMRYRTT
290
KDIILPFRVI
300
PLVREVGRTK
310
LEVKVVIKSN
320
FKPSLLAQKI
330
EVRIPTPLNT
340
SGVQVICMKG
350
KAKYKASENA
360
IVWKIKRMAG
370
MKESQISAEI
380
ELLPTNDKKK
390
WARPPISMNF
400
EVPFAPSGLK
410
VRYLKVFEPK
420
LNYSDHDVIK
430
WVRYIGRSGI
YETRC
Gene Ontology
| Classification |
GO ID |
Description |
| Cellular Component |
GO:0030121 |
AP-1 adaptor complex |
| Cellular Component |
GO:0030122 |
AP-2 adaptor complex |
| Cellular Component |
GO:0005905 |
clathrin-coated pit |
| Cellular Component |
GO:0005829 |
cytosol |
| Cellular Component |
GO:0098894 |
extrinsic component of presynaptic endocytic zone membrane |
| Cellular Component |
GO:0098978 |
glutamatergic synapse |
| Cellular Component |
GO:0005886 |
plasma membrane |
| Cellular Component |
GO:0098794 |
postsynapse |
| Cellular Component |
GO:0045202 |
synapse |
| Cellular Component |
GO:0008021 |
synaptic vesicle |
| Cellular Component |
GO:0043195 |
terminal bouton |
| Molecular Function |
GO:0035615 |
clathrin adaptor activity |
| Molecular Function |
GO:0097718 |
disordered domain specific binding |
| Molecular Function |
GO:0008289 |
lipid binding |
| Molecular Function |
GO:0050750 |
low-density lipoprotein particle receptor binding |
| Molecular Function |
GO:0005048 |
signal sequence binding |
| Molecular Function |
GO:0044325 |
transmembrane transporter binding |
| Biological Process |
GO:0072583 |
clathrin-dependent endocytosis |
| Biological Process |
GO:0006896 |
Golgi to vacuole transport |
| Biological Process |
GO:0006886 |
intracellular protein transport |
| Biological Process |
GO:1903077 |
negative regulation of protein localization to plasma membrane |
| Biological Process |
GO:0002092 |
positive regulation of receptor internalization |
| Biological Process |
GO:1900244 |
positive regulation of synaptic vesicle endocytosis |
| Biological Process |
GO:0098884 |
postsynaptic neurotransmitter receptor internalization |
| Biological Process |
GO:0065003 |
protein-containing complex assembly |
| Biological Process |
GO:0031623 |
receptor internalization |
| Biological Process |
GO:0097494 |
regulation of vesicle size |
| Biological Process |
GO:0048488 |
synaptic vesicle endocytosis |
| Biological Process |
GO:0006900 |
vesicle budding from membrane |
Reference
[1] Chen Y, Sun W, Sun Z, Zhao H, Wu T et al.. Effect of electroacupuncture on hippocampal protein lactylation in a rat model of vascular dementia.. Front Neurol 16:1629474. 2025. PMID: 40963935.