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Overview

Uniprot IDP97450
Protein NameATP synthase peripheral stalk subunit F6, mitochondrial
Gene NameAtp5pf
OrganismMus musculus

Kla Sites from experimental identification

Position Flanking peptide
105 PKFEVIDKPQS****
41 KELDPVQKLFVDKIR
46 VQKLFVDKIREYKSK
79 DRELYKLKQMYGKGE
84 KLKQMYGKGEMDTFP
94 MDTFPTFKFDDPKFE
99 TFKFDDPKFEVIDKP

Function

Subunit F6, of the mitochondrial membrane ATP synthase complex (F(1)F(0) ATP synthase or Complex V) that produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. ATP synthase complex consist of a soluble F(1) head domain - the catalytic core - and a membrane F(1) domain - the membrane proton channel. These two domains are linked by a central stalk rotating inside the F(1) region and a stationary peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation (By similarity). In vivo, can only synthesize ATP although its ATP hydrolase activity can be activated artificially in vitro (By similarity). Part of the complex F(0) domain (By similarity). Part of the complex F(0) domain and the peripheric stalk, which acts as a stator to hold the catalytic alpha(3)beta(3) subcomplex and subunit a/ATP6 static relative to the rotary elements (By similarity)

Protein Sequence

10 MVLQRIFRLS 20 SVLRSAVSVH 30 LKRNIGVTAV 40 AFNKELDPVQ 50 KLFVDKIREY 60 KSKRQASGGP 70 VDIGPEYQQD 80 LDRELYKLKQ 90 MYGKGEMDTF 100 PTFKFDDPKF EVIDKPQS

Gene Ontology

Classification GO ID Description
Cellular Component GO:0009986 cell surface
Cellular Component GO:0005814 centriole
Cellular Component GO:0005615 extracellular space
Cellular Component GO:0005743 mitochondrial inner membrane
Cellular Component GO:0005739 mitochondrion
Cellular Component GO:0045259 proton-transporting ATP synthase complex
Cellular Component GO:0097229 sperm end piece
Molecular Function GO:0044877 protein-containing complex binding
Molecular Function GO:0046933 proton-transporting ATP synthase activity, rotational mechanism
Biological Process GO:1900139 negative regulation of arachidonate secretion
Biological Process GO:0032307 negative regulation of prostaglandin secretion
Biological Process GO:0045777 positive regulation of blood pressure
Biological Process GO:0010460 positive regulation of heart rate
Biological Process GO:0042776 proton motive force-driven mitochondrial ATP synthesis

Reference

[1] Zhuo W, Zhang M, Tan J, Gao Y, Wang Y et al.. Lysine lactylation analysis of proteins in the heart of the Kawasaki disease mouse model.. Front Cell Dev Biol 13:1550220. 2025. PMID: 40114965.

[2] Wu D, Tang Y, Li X, Xiong S, Zhang Z et al.. Characterization of protein lactylation in healthy and ischemic mouse hearts.. Front Cardiovasc Med 12:1644886. 2025. PMID: 41089239.