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Overview

Uniprot ID	P97823
Protein Name	Acyl-protein thioesterase 1
Gene Name	Lypla1
Organism	Mus musculus

Kla Sites from experimental identification

Position	Flanking peptide
105	ALIDQEVKNGIPSNR

Function

Acts as an acyl-protein thioesterase (By similarity). Hydrolyzes fatty acids from S-acylated cysteine residues in proteins such as trimeric G alpha proteins or HRAS (By similarity). Acts as a palmitoyl thioesterase that catalyzes depalmitoylation of proteins, such as ADRB2, KCNMA1 and SQSTM1 (By similarity). Acts as a negative regulator of autophagy by mediating palmitoylation of SQSTM1, decreasing affinity between SQSTM1 and ATG8 proteins and recruitment of ubiquitinated cargo proteins to autophagosomes (By similarity). Acts as a lysophospholipase and hydrolyzes lysophosphatidylcholine (lyso-PC) (By similarity). Also hydrolyzes lysophosphatidylethanolamine (lyso-PE), lysophosphatidylinositol (lyso-PI) and lysophosphatidylserine (lyso-PS) (PubMed:9139730). Has much higher thioesterase activity than lysophospholipase activity (By similarity). Contributes to the production of lysophosphatidic acid (LPA) during blood coagulation by recognizing and cleaving plasma phospholipids to generate lysophospholipids which in turn act as substrates for ENPP2 to produce LPA (By similarity)

Protein Sequence

10 MCGNNMSAPM 20 PAVVPAARKA 30 TAAVIFLHGL 40 GDTGHGWAEA 50 FAGIKSPHIK 60 YICPHAPVMP 70 VTLNMNMAMP 80 SWFDIVGLSP 90 DSQEDESGIK 100 QAAETVKALI 110 DQEVKNGIPS 120 NRIILGGFSQ 130 GGALSLYTAL 140 TTQQKLAGVT 150 ALSCWLPLRA 160 SFSQGPINSA 170 NRDISVLQCH 180 GDCDPLVPLM 190 FGSLTVERLK 200 ALINPANVTF 210 KIYEGMMHSS 220 CQQEMMDVKH 230 FIDKLLPPID

Gene Ontology

Classification	GO ID	Description
Cellular Component	GO:0005737	cytoplasm
Cellular Component	GO:0005829	cytosol
Cellular Component	GO:0005783	endoplasmic reticulum
Cellular Component	GO:0005739	mitochondrion
Cellular Component	GO:0031965	nuclear membrane
Cellular Component	GO:0005654	nucleoplasm
Cellular Component	GO:0005886	plasma membrane
Molecular Function	GO:0004620	glycerophospholipase activity
Molecular Function	GO:0016298	lipase activity
Molecular Function	GO:0008474	palmitoyl-(protein) hydrolase activity
Molecular Function	GO:0004622	phosphatidylcholine lysophospholipase A1 activity
Biological Process	GO:0035973	aggrephagy
Biological Process	GO:0006631	fatty acid metabolic process
Biological Process	GO:0015908	fatty acid transport
Biological Process	GO:1905336	negative regulation of aggrephagy
Biological Process	GO:0042997	negative regulation of Golgi to plasma membrane protein transport
Biological Process	GO:0002084	protein depalmitoylation
Biological Process	GO:0071211	protein targeting to vacuole involved in autophagy

Reference

[1] Zhuo W, Zhang M, Tan J, Gao Y, Wang Y et al.. Lysine lactylation analysis of proteins in the heart of the Kawasaki disease mouse model.. Front Cell Dev Biol 13:1550220. 2025. PMID: 40114965.

[2] Wu D, Tang Y, Li X, Xiong S, Zhang Z et al.. Characterization of protein lactylation in healthy and ischemic mouse hearts.. Front Cardiovasc Med 12:1644886. 2025. PMID: 41089239.