Overview
| Uniprot ID | P98187 |
|---|
| Protein Name | Cytochrome P450 4F8 |
|---|
| Gene Name | CYP4F8 |
|---|
| Organism | Homo sapiens |
|---|
Kla Sites from experimental identification
| Position |
Flanking peptide |
| 308 |
VLLLSEDKNGKELSD |
| 452 |
FDPENAQKRSPMAFI |
Function
A cytochrome P450 monooxygenase involved in the metabolism of endogenous polyunsaturated fatty acids (PUFAs) and their oxygenated derivatives (oxylipins). Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (CPR; NADPH-ferrihemoprotein reductase). Catalyzes the hydroxylation of carbon hydrogen bonds, with preference for omega-1 and omega-2 positions (PubMed:10791960, PubMed:15789615, PubMed:16112640). Hydroxylates (5Z,8Z,11Z,14Z)-eicosatetraenoic acid (arachidonate) predominantly at omega-2 position to form (18R)-hydroxyeicosatetraenoic acid (18R-HETE) (PubMed:10791960). Exhibits omega-1 hydroxylase activity toward prostaglandin (PG) H1, PGH2 and PGI2 (PubMed:10791960, PubMed:15789615). Catalyzes the epoxidation of double bonds of PUFAs, including docosahexaenoic and docosapentaenoic acids (PubMed:16112640). Shows little activity against PGD2, PGE1, PGE2, PGF2alpha, and leukotriene B4
Protein Sequence
10
MSLLSLSWLG
20
LRPVAASPWL
30
LLLVVGASWL
40
LARILAWTYA
50
FYHNGRRLRC
60
FPQPRKQNWF
70
LGHLGLVTPT
80
EEGLRVLTQL
90
VATYPQGFVR
100
WLGPITPIIN
110
LCHPDIVRSV
120
INTSDAITDK
130
DIVFYKTLKP
140
WLGDGLLLSV
150
GDKWRHHRRL
160
LTPAFHFNIL
170
KPYIKIFSKS
180
ANIMHAKWQR
190
LAMEGSTCLD
200
VFEHISLMTL
210
DSLQKCIFSF
220
DSNCQEKPSE
230
YITAIMELSA
240
LVVKRNNQFF
250
RYKDFLYFLT
260
PCGRRFHRAC
270
RLVHDFTDAV
280
IQERRRTLTS
290
QGVDDFLQAK
300
AKSKTLDFID
310
VLLLSEDKNG
320
KELSDEDIRA
330
EADTFMFGGH
340
DTTASGLSWV
350
LYNLARHPEY
360
QERCRQEVQE
370
LLKDREPKEI
380
EWDDLAQLPF
390
LTMCLKESLR
400
LHPPIPTFAR
410
GCTQDVVLPD
420
SRVIPKGNVC
430
NINIFAIHHN
440
PSVWPDPEVY
450
DPFRFDPENA
460
QKRSPMAFIP
470
FSAGPRNCIG
480
QKFAMAEMKV
490
VLALTLLRFR
500
ILPDHREPRR
510
TPEIVLRAED
520
GLWLRVEPLG
Gene Ontology
| Classification |
GO ID |
Description |
| Cellular Component |
GO:0005789 |
endoplasmic reticulum membrane |
| Molecular Function |
GO:0018685 |
alkane 1-monooxygenase activity |
| Molecular Function |
GO:0020037 |
heme binding |
| Molecular Function |
GO:0005506 |
iron ion binding |
| Molecular Function |
GO:0004497 |
monooxygenase activity |
| Molecular Function |
GO:0016712 |
oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen |
| Biological Process |
GO:0019369 |
arachidonate metabolic process |
| Biological Process |
GO:0006690 |
icosanoid metabolic process |
| Biological Process |
GO:0042361 |
menaquinone catabolic process |
| Biological Process |
GO:0042376 |
phylloquinone catabolic process |
| Biological Process |
GO:0006693 |
prostaglandin metabolic process |
Reference
[1] Shi CM, Wang QC, Li XL, Yang YH, Tang XY et al.. Global Profiling of Protein Lactylation in Human Hippocampi.. Proteomics Clin Appl 19(2):e202400061. 2025 Mar. PMID: 39610256.