Search Results

Overview

Uniprot ID	Q00059
Protein Name	Transcription factor A, mitochondrial
Gene Name	TFAM
Organism	Homo sapiens

Kla Sites from experimental identification

Position	Flanking peptide
174	AERFQEAKGDSPQEK
205	ELYIQHAKEDETRYH

Function

Binds to the mitochondrial light strand promoter and functions in mitochondrial transcription regulation (PubMed:29445193, PubMed:32183942). Component of the mitochondrial transcription initiation complex, composed at least of TFB2M, TFAM and POLRMT that is required for basal transcription of mitochondrial DNA (PubMed:29149603). In this complex, TFAM recruits POLRMT to a specific promoter whereas TFB2M induces structural changes in POLRMT to enable promoter opening and trapping of the DNA non-template strand (PubMed:20410300). Required for accurate and efficient promoter recognition by the mitochondrial RNA polymerase (PubMed:22037172). Promotes transcription initiation from the HSP1 and the light strand promoter by binding immediately upstream of transcriptional start sites (PubMed:22037172). Is able to unwind DNA (PubMed:22037172). Bends the mitochondrial light strand promoter DNA into a U-turn shape via its HMG boxes (PubMed:1737790). Required for maintenance of normal levels of mitochondrial DNA (PubMed:19304746, PubMed:22841477). May play a role in organizing and compacting mitochondrial DNA (PubMed:22037171)

Protein Sequence

10 MAFLRSMWGV 20 LSALGRSGAE 30 LCTGCGSRLR 40 SPFSFVYLPR 50 WFSSVLASCP 60 KKPVSSYLRF 70 SKEQLPIFKA 80 QNPDAKTTEL 90 IRRIAQRWRE 100 LPDSKKKIYQ 110 DAYRAEWQVY 120 KEEISRFKEQ 130 LTPSQIMSLE 140 KEIMDKHLKR 150 KAMTKKKELT 160 LLGKPKRPRS 170 AYNVYVAERF 180 QEAKGDSPQE 190 KLKTVKENWK 200 NLSDSEKELY 210 IQHAKEDETR 220 YHNEMKSWEE 230 QMIEVGRKDL 240 LRRTIKKQRK YGAEEC

Gene Ontology

Classification	GO ID	Description
Cellular Component	GO:0005829	cytosol
Cellular Component	GO:0005759	mitochondrial matrix
Cellular Component	GO:0042645	mitochondrial nucleoid
Cellular Component	GO:0005739	mitochondrion
Cellular Component	GO:0005634	nucleus
Cellular Component	GO:0032991	protein-containing complex
Molecular Function	GO:0003682	chromatin binding
Molecular Function	GO:0031072	heat shock protein binding
Molecular Function	GO:0001018	mitochondrial promoter sequence-specific DNA binding
Molecular Function	GO:0034246	mitochondrial transcription factor activity
Molecular Function	GO:0003723	RNA binding
Molecular Function	GO:0043565	sequence-specific DNA binding
Molecular Function	GO:0001223	transcription coactivator binding
Biological Process	GO:0006390	mitochondrial transcription
Biological Process	GO:0001666	response to hypoxia
Biological Process	GO:0007584	response to nutrient
Biological Process	GO:0006391	transcription initiation at mitochondrial promoter

Reference

[1] Yang Z, Yan C, Ma J, Peng P, Ren X et al.. Lactylome analysis suggests lactylation-dependent mechanisms of metabolic adaptation in hepatocellular carcinoma.. Nat Metab 5(1):61-79. 2023 Jan. PMID: 36593272.

[2] Shi CM, Wang QC, Li XL, Yang YH, Tang XY et al.. Global Profiling of Protein Lactylation in Human Hippocampi.. Proteomics Clin Appl 19(2):e202400061. 2025 Mar. PMID: 39610256.