Search Results
Overview
| Uniprot ID | Q00403 |
|---|---|
| Protein Name | Transcription initiation factor IIB |
| Gene Name | GTF2B |
| Organism | Homo sapiens |
Kla Sites from experimental identification
| Position | Flanking peptide |
|---|---|
| 100 | FDEFGNSKYQNRRTM |
| 59 | WRTFSNDKATKDPSR |
| 62 | FSNDKATKDPSRVGD |
Function
General transcription factor that plays a role in transcription initiation by RNA polymerase II (Pol II). Involved in the pre-initiation complex (PIC) formation and Pol II recruitment at promoter DNA (PubMed:12931194, PubMed:1517211, PubMed:1876184, PubMed:1946368, PubMed:27193682, PubMed:3029109, PubMed:3818643, PubMed:7601352, PubMed:8413225, PubMed:8515820, PubMed:8516311, PubMed:8516312, PubMed:9420329). Together with the TATA box-bound TBP forms the core initiation complex and provides a bridge between TBP and the Pol II-TFIIF complex (PubMed:8413225, PubMed:8504927, PubMed:8515820, PubMed:8516311, PubMed:8516312). Released from the PIC early following the onset of transcription during the initiation and elongation transition and reassociates with TBP during the next transcription cycle (PubMed:7601352). Associates with chromatin to core promoter-specific regions (PubMed:12931194, PubMed:24441171). Binds to two distinct DNA core promoter consensus sequence elements in a TBP-independent manner; these IIB-recognition elements (BREs) are localized immediately upstream (BREu), 5'-[GC][GC][GA]CGCC-3', and downstream (BREd), 5'-[GA]T[TGA][TG][GT][TG][TG]-3', of the TATA box element (PubMed:10619841, PubMed:16230532, PubMed:7675079, PubMed:9420329). Modulates transcription start site selection (PubMed:10318856). Also exhibits autoacetyltransferase activity that contributes to the activated transcription (PubMed:12931194)
Protein Sequence
Gene Ontology
| Classification | GO ID | Description |
|---|---|---|
| Cellular Component | GO:0032153 | cell division site |
| Molecular Function | GO:0061733 | protein-lysine-acetyltransferase activity |
| Molecular Function | GO:0000993 | RNA polymerase II complex binding |
| Molecular Function | GO:0000979 | RNA polymerase II core promoter sequence-specific DNA binding |
| Molecular Function | GO:0016251 | RNA polymerase II general transcription initiation factor activity |
| Molecular Function | GO:0017025 | TBP-class protein binding |
| Molecular Function | GO:0008270 | zinc ion binding |
| Biological Process | GO:0006352 | DNA-templated transcription initiation |
| Biological Process | GO:0051177 | meiotic sister chromatid cohesion |
| Biological Process | GO:1904798 | positive regulation of core promoter binding |
| Biological Process | GO:0006473 | protein acetylation |
| Biological Process | GO:1990114 | RNA polymerase II core complex assembly |
| Biological Process | GO:0051123 | RNA polymerase II preinitiation complex assembly |
| Biological Process | GO:0051225 | spindle assembly |
| Biological Process | GO:0006366 | transcription by RNA polymerase II |
| Biological Process | GO:0006367 | transcription initiation at RNA polymerase II promoter |
| Biological Process | GO:0001174 | transcriptional start site selection at RNA polymerase II promoter |
| Biological Process | GO:0019083 | viral transcription |
| Cellular Component | GO:0005694 | chromosome |
| Cellular Component | GO:0042585 | germinal vesicle |
| Cellular Component | GO:0000776 | kinetochore |
| Cellular Component | GO:0016604 | nuclear body |
| Cellular Component | GO:0005654 | nucleoplasm |
| Cellular Component | GO:0005634 | nucleus |
| Cellular Component | GO:0032993 | protein-DNA complex |
| Cellular Component | GO:0090575 | RNA polymerase II transcription regulator complex |
| Cellular Component | GO:0005669 | transcription factor TFIID complex |
| Cellular Component | GO:0097550 | transcription preinitiation complex |
| Molecular Function | GO:0016407 | acetyltransferase activity |
| Molecular Function | GO:0140297 | DNA-binding transcription factor binding |
| Molecular Function | GO:0046966 | nuclear thyroid hormone receptor binding |
| Molecular Function | GO:1990841 | promoter-specific chromatin binding |
Reference
[1] Yang Z, Yan C, Ma J, Peng P, Ren X et al.. Lactylome analysis suggests lactylation-dependent mechanisms of metabolic adaptation in hepatocellular carcinoma.. Nat Metab 5(1):61-79. 2023 Jan. PMID: 36593272.
[2] He C, Zhang J, Bai X, Lu C, Zhang K. Lysine lactylation-based insight to understanding the characterization of cervical cancer.. Biochim Biophys Acta Mol Basis Dis 1870(7):167356. 2024 Oct. PMID: 39025375.