Search Results

Overview

Uniprot IDQ01085
Protein NameNucleolysin TIAR
Gene NameTIAL1
OrganismHomo sapiens

Kla Sites from experimental identification

Position Flanking peptide
189 STQENNTKQLRFEDV
90 ATTPSSQKKDTSNHF

Function

RNA-binding protein involved in alternative pre-RNA splicing and in cytoplasmic stress granules formation (PubMed:10613902, PubMed:1326761, PubMed:17488725, PubMed:8576255). Shows a preference for uridine-rich RNAs (PubMed:8576255). Activates splicing of alternative exons with weak 5' splice sites followed by a U-rich stretch on its own pre-mRNA and on TIA1 mRNA (By similarity). Promotes the inclusion of TIA1 exon 5 to give rise to the long isoform (isoform a) of TIA1 (PubMed:17488725). Acts downstream of the stress-induced phosphorylation of EIF2S1/EIF2A to promote the recruitment of untranslated mRNAs to cytoplasmic stress granules (SG) (PubMed:10613902). Possesses nucleolytic activity against cytotoxic lymphocyte target cells (PubMed:1326761). May be involved in apoptosis (PubMed:1326761)

Protein Sequence

10 MMEDDGQPRT 20 LYVGNLSRDV 30 TEVLILQLFS 40 QIGPCKSCKM 50 ITEHTSNDPY 60 CFVEFYEHRD 70 AAAALAAMNG 80 RKILGKEVKV 90 NWATTPSSQK 100 KDTSNHFHVF 110 VGDLSPEITT 120 EDIKSAFAPF 130 GKISDARVVK 140 DMATGKSKGY 150 GFVSFYNKLD 160 AENAIVHMGG 170 QWLGGRQIRT 180 NWATRKPPAP 190 KSTQENNTKQ 200 LRFEDVVNQS 210 SPKNCTVYCG 220 GIASGLTDQL 230 MRQTFSPFGQ 240 IMEIRVFPEK 250 GYSFVRFSTH 260 ESAAHAIVSV 270 NGTTIEGHVV 280 KCYWGKESPD 290 MTKNFQQVDY 300 SQWGQWSQVY 310 GNPQQYGQYM 320 ANGWQVPPYG 330 VYGQPWNQQG 340 FGVDQSPSAA 350 WMGGFGAQPP 360 QGQAPPPVIP 370 PPNQAGYGMA SYQTQ

Gene Ontology

Classification GO ID Description
Cellular Component GO:0044194 cytolytic granule
Cellular Component GO:0005737 cytoplasm
Cellular Component GO:0010494 cytoplasmic stress granule
Cellular Component GO:0005829 cytosol
Cellular Component GO:0005764 lysosome
Cellular Component GO:0005654 nucleoplasm
Cellular Component GO:0005634 nucleus
Molecular Function GO:0003677 DNA binding
Molecular Function GO:0003730 mRNA 3'-UTR binding
Molecular Function GO:0140517 protein-RNA adaptor activity
Molecular Function GO:0003723 RNA binding
Biological Process GO:0006915 apoptotic process
Biological Process GO:0006952 defense response
Biological Process GO:0008285 negative regulation of cell population proliferation
Biological Process GO:0035332 positive regulation of hippo signaling
Biological Process GO:2000637 positive regulation of miRNA-mediated gene silencing
Biological Process GO:0000381 regulation of alternative mRNA splicing, via spliceosome
Biological Process GO:0006357 regulation of transcription by RNA polymerase II

Reference

[1] Yang Z, Yan C, Ma J, Peng P, Ren X et al.. Lactylome analysis suggests lactylation-dependent mechanisms of metabolic adaptation in hepatocellular carcinoma.. Nat Metab 5(1):61-79. 2023 Jan. PMID: 36593272.

[2] Hong H, Chen X, Wang H, Gu X, Yuan Y et al.. Global profiling of protein lysine lactylation and potential target modified protein analysis in hepatocellular carcinoma.. Proteomics 23(9):e2200432. 2023 May. PMID: 36625413.