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Overview

Uniprot ID	Q01484
Protein Name	Ankyrin-2
Gene Name	ANK2
Organism	Homo sapiens

Kla Sites from experimental identification

Position	Flanking peptide
2033	KVRVEKEKGPILTQR
206	AARKDDTKSAALLLQ
3464	TSPTKESKEHFFDLY
3746	ALFPQTHKEQVQQDF
618	VALLLLEKGASPHAT

Function

Plays an essential role in the localization and membrane stabilization of ion transporters and ion channels in several cell types, including cardiomyocytes, as well as in striated muscle cells. In skeletal muscle, required for proper localization of DMD and DCTN4 and for the formation and/or stability of a special subset of microtubules associated with costameres and neuromuscular junctions. In cardiomyocytes, required for coordinate assembly of Na/Ca exchanger, SLC8A1/NCX1, Na/K ATPases ATP1A1 and ATP1A2 and inositol 1,4,5-trisphosphate (InsP3) receptors at sarcoplasmic reticulum/sarcolemma sites. Required for expression and targeting of SPTBN1 in neonatal cardiomyocytes and for the regulation of neonatal cardiomyocyte contraction rate (PubMed:12571597). In the inner segment of rod photoreceptors, required for the coordinated expression of the Na/K ATPase, Na/Ca exchanger and beta-2-spectrin (SPTBN1) (By similarity). Plays a role in endocytosis and intracellular protein transport. Associates with phosphatidylinositol 3-phosphate (PI3P)-positive organelles and binds dynactin to promote long-range motility of cells. Recruits RABGAP1L to (PI3P)-positive early endosomes, where RABGAP1L inactivates RAB22A, and promotes polarized trafficking to the leading edge of the migrating cells. Part of the ANK2/RABGAP1L complex which is required for the polarized recycling of fibronectin receptor ITGA5 ITGB1 to the plasma membrane that enables continuous directional cell migration (By similarity)

Protein Sequence

10 MMNEDAAQKS 20 DSGEKFNGSS 30 QRRKRPKKSD 40 SNASFLRAAR 50 AGNLDKVVEY 60 LKGGIDINTC 70 NQNGLNALHL 80 AAKEGHVGLV 90 QELLGRGSSV 100 DSATKKGNTA 110 LHIASLAGQA 120 EVVKVLVKEG 130 ANINAQSQNG 140 FTPLYMAAQE 150 NHIDVVKYLL 160 ENGANQSTAT 170 EDGFTPLAVA 180 LQQGHNQAVA 190 ILLENDTKGK 200 VRLPALHIAA 210 RKDDTKSAAL 220 LLQNDHNADV 230 QSKMMVNRTT 240 ESGFTPLHIA 250 AHYGNVNVAT 260 LLLNRGAAVD 270 FTARNGITPL 280 HVASKRGNTN 290 MVKLLLDRGG 300 QIDAKTRDGL 310 TPLHCAARSG 320 HDQVVELLLE 330 RGAPLLARTK 340 NGLSPLHMAA 350 QGDHVECVKH 360 LLQHKAPVDD 370 VTLDYLTALH 380 VAAHCGHYRV 390 TKLLLDKRAN 400 PNARALNGFT 410 PLHIACKKNR 420 IKVMELLVKY 430 GASIQAITES 440 GLTPIHVAAF 450 MGHLNIVLLL 460 LQNGASPDVT 470 NIRGETALHM 480 AARAGQVEVV 490 RCLLRNGALV 500 DARAREEQTP 510 LHIASRLGKT 520 EIVQLLLQHM 530 AHPDAATTNG 540 YTPLHISARE 550 GQVDVASVLL 560 EAGAAHSLAT 570 KKGFTPLHVA 580 AKYGSLDVAK 590 LLLQRRAAAD 600 SAGKNGLTPL 610 HVAAHYDNQK 620 VALLLLEKGA 630 SPHATAKNGY 640 TPLHIAAKKN 650 QMQIASTLLN 660 YGAETNIVTK 670 QGVTPLHLAS 680 QEGHTDMVTL 690 LLDKGANIHM 700 STKSGLTSLH 710 LAAQEDKVNV 720 ADILTKHGAD 730 QDAHTKLGYT 740 PLIVACHYGN 750 VKMVNFLLKQ 760 GANVNAKTKN 770 GYTPLHQAAQ 780 QGHTHIINVL 790 LQHGAKPNAT 800 TANGNTALAI 810 AKRLGYISVV 820 DTLKVVTEEV 830 TTTTTTITEK 840 HKLNVPETMT 850 EVLDVSDEEG 860 DDTMTGDGGE 870 YLRPEDLKEL 880 GDDSLPSSQF 890 LDGMNYLRYS 900 LEGGRSDSLR 910 SFSSDRSHTL 920 SHASYLRDSA 930 VMDDSVVIPS 940 HQVSTLAKEA 950 ERNSYRLSWG 960 TENLDNVALS 970 SSPIHSGFLV 980 SFMVDARGGA 990 MRGCRHNGLR 1000 IIIPPRKCTA 1010 PTRVTCRLVK 1020 RHRLATMPPM 1030 VEGEGLASRL 1040 IEVGPSGAQF 1050 LGKLHLPTAP 1060 PPLNEGESLV 1070 SRILQLGPPG 1080 TKFLGPVIVE 1090 IPHFAALRGK 1100 ERELVVLRSE 1110 NGDSWKEHFC 1120 DYTEDELNEI 1130 LNGMDEVLDS 1140 PEDLEKKRIC 1150 RIITRDFPQY 1160 FAVVSRIKQD 1170 SNLIGPEGGV 1180 LSSTVVPQVQ 1190 AVFPEGALTK 1200 RIRVGLQAQP 1210 MHSELVKKIL 1220 GNKATFSPIV 1230 TLEPRRRKFH 1240 KPITMTIPVP 1250 KASSDVMLNG 1260 FGGDAPTLRL 1270 LCSITGGTTP 1280 AQWEDITGTT 1290 PLTFVNECVS 1300 FTTNVSARFW 1310 LIDCRQIQES 1320 VTFASQVYRE 1330 IICVPYMAKF 1340 VVFAKSHDPI 1350 EARLRCFCMT 1360 DDKVDKTLEQ 1370 QENFAEVARS 1380 RDVEVLEGKP 1390 IYVDCFGNLV 1400 PLTKSGQHHI 1410 FSFFAFKENR 1420 LPLFVKVRDT 1430 TQEPCGRLSF 1440 MKEPKSTRGL 1450 VHQAICNLNI 1460 TLPIYTKESE 1470 SDQEQEEEID 1480 MTSEKNDETE 1490 STETSVLKSH 1500 LVNEVPVLAS 1510 PDLLSEVSEM 1520 KQDLIKMTAI 1530 LTTDVSDKAG 1540 SIKVKELVKA 1550 AEEEPGEPFE 1560 IVERVKEDLE 1570 KVNEILRSGT 1580 CTRDESSVQS 1590 SRSERGLVEE 1600 EWVIVSDEEI 1610 EEARQKAPLE 1620 ITEYPCVEVR 1630 IDKEIKGKVE 1640 KDSTGLVNYL 1650 TDDLNTCVPL 1660 PKEQLQTVQD 1670 KAGKKCEALA 1680 VGRSSEKEGK 1690 DIPPDETQST 1700 QKQHKPSLGI 1710 KKPVRRKLKE 1720 KQKQKEEGLQ 1730 ASAEKAELKK 1740 GSSEESLGED 1750 PGLAPEPLPT 1760 VKATSPLIEE 1770 TPIGSIKDKV 1780 KALQKRVEDE 1790 QKGRSKLPIR 1800 VKGKEDVPKK 1810 TTHRPHPAAS 1820 PSLKSERHAP 1830 GSPSPKTERH 1840 STLSSSAKTE 1850 RHPPVSPSSK 1860 TEKHSPVSPS 1870 AKTERHSPAS 1880 SSSKTEKHSP 1890 VSPSTKTERH 1900 SPVSSTKTER 1910 HPPVSPSGKT 1920 DKRPPVSPSG 1930 RTEKHPPVSP 1940 GRTEKRLPVS 1950 PSGRTDKHQP 1960 VSTAGKTEKH 1970 LPVSPSGKTE 1980 KQPPVSPTSK 1990 TERIEETMSV 2000 RELMKAFQSG 2010 QDPSKHKTGL 2020 FEHKSAKQKQ 2030 PQEKGKVRVE 2040 KEKGPILTQR 2050 EAQKTENQTI 2060 KRGQRLPVTG 2070 TAESKRGVRV 2080 SSIGVKKEDA 2090 AGGKEKVLSH 2100 KIPEPVQSVP 2110 EEESHRESEV 2120 PKEKMADEQG 2130 DMDLQISPDR 2140 KTSTDFSEVI 2150 KQELEDNDKY 2160 QQFRLSEETE 2170 KAQLHLDQVL 2180 TSPFNTTFPL 2190 DYMKDEFLPA 2200 LSLQSGALDG 2210 SSESLKNEGV 2220 AGSPCGSLME 2230 GTPQISSEES 2240 YKHEGLAETP 2250 ETSPESLSFS 2260 PKKSEEQTGE 2270 TKESTKTETT 2280 TEIRSEKEHP 2290 TTKDITGGSE 2300 ERGATVTEDS 2310 ETSTESFQKE 2320 ATLGSPKDTS 2330 PKRQDDCTGS 2340 CSVALAKETP 2350 TGLTEEAACD 2360 EGQRTFGSSA 2370 HKTQTDSEVQ 2380 ESTATSDETK 2390 ALPLPEASVK 2400 TDTGTESKPQ 2410 GVIRSPQGLE 2420 LALPSRDSEV 2430 LSAVADDSLA 2440 VSHKDSLEAS 2450 PVLEDNSSHK 2460 TPDSLEPSPL 2470 KESPCRDSLE 2480 SSPVEPKMKA 2490 GIFPSHFPLP 2500 AAVAKTELLT 2510 EVASVRSRLL 2520 RDPDGSAEDD 2530 SLEQTSLMES 2540 SGKSPLSPDT 2550 PSSEEVSYEV 2560 TPKTTDVSTP 2570 KPAVIHECAE 2580 EDDSENGEKK 2590 RFTPEEEMFK 2600 MVTKIKMFDE 2610 LEQEAKQKRD 2620 YKKEPKQEES 2630 SSSSDPDADC 2640 SVDVDEPKHT 2650 GSGEDESGVP 2660 VLVTSESRKV 2670 SSSSESEPEL 2680 AQLKKGADSG 2690 LLPEPVIRVQ 2700 PPSPLPSSMD 2710 SNSSPEEVQF 2720 QPVVSKQYTF 2730 KMNEDTQEEP 2740 GKSEEEKDSE 2750 SHLAEDRHAV 2760 STEAEDRSYD 2770 KLNRDTDQPK 2780 ICDGHGCEAM 2790 SPSSSAAPVS 2800 SGLQSPTGDD 2810 VDEQPVIYKE 2820 SLALQGTHEK 2830 DTEGEELDVS 2840 RAESPQADCP 2850 SESFSSSSSL 2860 PHCLVSEGKE 2870 LDEDISATSS 2880 IQKTEVTKTD 2890 ETFENLPKDC 2900 PSQDSSITTQ 2910 TDRFSMDVPV 2920 SDLAENDEIY 2930 DPQITSPYEN 2940 VPSQSFFSSE 2950 ESKTQTDANH 2960 TTSFHSSEVY 2970 SVTITSPVED 2980 VVVASSSSGT 2990 VLSKESNFEG 3000 QDIKMESQQE 3010 STLWEMQSDS 3020 VSSSFEPTMS 3030 ATTTVVGEQI 3040 SKVIITKTDV 3050 DSDSWSEIRE 3060 DDEAFEARVK 3070 EEEQKIFGLM 3080 VDRQSQGTTP 3090 DTTPARTPTE 3100 EGTPTSEQNP 3110 FLFQEGKLFE 3120 MTRSGAIDMT 3130 KRSYADESFH 3140 FFQIGQESRE 3150 ETLSEDVKEG 3160 ATGADPLPLE 3170 TSAESLALSE 3180 SKETVDDEAD 3190 LLPDDVSEEV 3200 EEIPASDAQL 3210 NSQMGISAST 3220 ETPTKEAVSV 3230 GTKDLPTVQT 3240 GDIPPLSGVK 3250 QISCPDSSEP 3260 AVQVQLDFST 3270 LTRSVYSDRG 3280 DDSPDSSPEE 3290 QKSVIEIPTA 3300 PMENVPFTES 3310 KSKIPVRTMP 3320 TSTPAPPSAE 3330 YESSVSEDFL 3340 SSVDEENKAD 3350 EAKPKSKLPV 3360 KVPLQRVEQQ 3370 LSDLDTSVQK 3380 TVAPQGQDMA 3390 SIAPDNRSKS 3400 ESDASSLDSK 3410 TKCPVKTRSY 3420 TETETESRER 3430 AEELELESEE 3440 GATRPKILTS 3450 RLPVKSRSTT 3460 SSCRGGTSPT 3470 KESKEHFFDL 3480 YRNSIEFFEE 3490 ISDEASKLVD 3500 RLTQSEREQE 3510 IVSDDESSSA 3520 LEVSVIENLP 3530 PVETEHSVPE 3540 DIFDTRPIWD 3550 ESIETLIERI 3560 PDENGHDHAE 3570 DPQDEQERIE 3580 ERLAYIADHL 3590 GFSWTELARE 3600 LDFTEEQIHQ 3610 IRIENPNSLQ 3620 DQSHALLKYW 3630 LERDGKHATD 3640 TNLVECLTKI 3650 NRMDIVHLME 3660 TNTEPLQERI 3670 SHSYAEIEQT 3680 ITLDHSEGFS 3690 VLQEELCTAQ 3700 HKQKEEQAVS 3710 KESETCDHPP 3720 IVSEEDISVG 3730 YSTFQDGVPK 3740 TEGDSSATAL 3750 FPQTHKEQVQ 3760 QDFSGKMQDL 3770 PEESSLEYQQ 3780 EYFVTTPGTE 3790 TSETQKAMIV 3800 PSSPSKTPEE 3810 VSTPAEEEKL 3820 YLQTPTSSER 3830 GGSPIIQEPE 3840 EPSEHREESS 3850 PRKTSLVIVE 3860 SADNQPETCE 3870 RLDEDAAFEK 3880 GDDMPEIPPE 3890 TVTEEEYIDE 3900 HGHTVVKKVT 3910 RKIIRRYVSS 3920 EGTEKEEIMV 3930 QGMPQEPVNI 3940 EEGDGYSKVI 3950 KRVVLKSDTE QSEDNNE

Gene Ontology

Classification	GO ID	Description
Cellular Component	GO:0031672	A band
Biological Process	GO:0086066	atrial cardiac muscle cell to AV node cell communication
Biological Process	GO:0003283	atrial septum development
Biological Process	GO:0006897	endocytosis
Biological Process	GO:0006874	intracellular calcium ion homeostasis
Biological Process	GO:0008104	intracellular protein localization
Biological Process	GO:0086046	membrane depolarization during SA node cell action potential
Biological Process	GO:0030913	paranodal junction assembly
Biological Process	GO:0051928	positive regulation of calcium ion transport
Biological Process	GO:0010628	positive regulation of gene expression
Biological Process	GO:1903288	positive regulation of potassium ion import across plasma membrane
Biological Process	GO:0034394	protein localization to cell surface
Biological Process	GO:0070972	protein localization to endoplasmic reticulum
Biological Process	GO:0036309	protein localization to M-band
Biological Process	GO:0072659	protein localization to plasma membrane
Biological Process	GO:0036371	protein localization to T-tubule
Biological Process	GO:0050821	protein stabilization
Biological Process	GO:0015031	protein transport
Biological Process	GO:0098910	regulation of atrial cardiac muscle cell action potential
Biological Process	GO:0051924	regulation of calcium ion transport
Biological Process	GO:0086004	regulation of cardiac muscle cell contraction
Biological Process	GO:0055117	regulation of cardiac muscle contraction
Biological Process	GO:0010882	regulation of cardiac muscle contraction by calcium ion signaling
Biological Process	GO:0010881	regulation of cardiac muscle contraction by regulation of the release of sequestered calcium ion
Biological Process	GO:0002027	regulation of heart rate
Biological Process	GO:0086091	regulation of heart rate by cardiac conduction
Biological Process	GO:0031647	regulation of protein stability
Biological Process	GO:0051279	regulation of release of sequestered calcium ion into cytosol
Biological Process	GO:0098907	regulation of SA node cell action potential
Biological Process	GO:0060307	regulation of ventricular cardiac muscle cell membrane repolarization
Biological Process	GO:0051597	response to methylmercury
Biological Process	GO:0086015	SA node cell action potential
Biological Process	GO:0086070	SA node cell to atrial cardiac muscle cell communication
Biological Process	GO:0070296	sarcoplasmic reticulum calcium ion transport
Biological Process	GO:0033292	T-tubule organization
Biological Process	GO:0086005	ventricular cardiac muscle cell action potential
Cellular Component	GO:0016324	apical plasma membrane
Cellular Component	GO:0016323	basolateral plasma membrane
Cellular Component	GO:0043034	costamere
Cellular Component	GO:0005856	cytoskeleton
Cellular Component	GO:0005829	cytosol
Cellular Component	GO:0005769	early endosome
Cellular Component	GO:0014704	intercalated disc
Cellular Component	GO:0005764	lysosome
Cellular Component	GO:0031430	M band
Cellular Component	GO:0005739	mitochondrion
Cellular Component	GO:0043005	neuron projection
Cellular Component	GO:0005886	plasma membrane
Cellular Component	GO:0045211	postsynaptic membrane
Cellular Component	GO:0055037	recycling endosome
Cellular Component	GO:0042383	sarcolemma
Cellular Component	GO:0030315	T-tubule
Cellular Component	GO:0030018	Z disc
Molecular Function	GO:0051117	ATPase binding
Molecular Function	GO:0099103	channel activator activity
Molecular Function	GO:0008093	cytoskeletal anchor activity
Molecular Function	GO:0019899	enzyme binding
Molecular Function	GO:0140031	phosphorylation-dependent protein binding
Molecular Function	GO:0099104	potassium channel activator activity
Molecular Function	GO:0019901	protein kinase binding
Molecular Function	GO:0030674	protein-macromolecule adaptor activity
Molecular Function	GO:0030507	spectrin binding
Molecular Function	GO:0005200	structural constituent of cytoskeleton
Molecular Function	GO:0044325	transmembrane transporter binding
Biological Process	GO:0086014	atrial cardiac muscle cell action potential

Reference

[1] Shi CM, Wang QC, Li XL, Yang YH, Tang XY et al.. Global Profiling of Protein Lactylation in Human Hippocampi.. Proteomics Clin Appl 19(2):e202400061. 2025 Mar. PMID: 39610256.