Overview
| Uniprot ID | Q01650 |
|---|
| Protein Name | Large neutral amino acids transporter small subunit 1 |
|---|
| Gene Name | SLC7A5 |
|---|
| Organism | Homo sapiens |
|---|
Kla Sites from experimental identification
| Position |
Flanking peptide |
| 479 |
YFFGVWWKNKPKWLL |
Function
The heterodimer with SLC3A2 functions as a sodium-independent, high-affinity transporter that mediates uptake of large neutral amino acids such as phenylalanine, tyrosine, leucine, histidine, methionine, tryptophan, valine, isoleucine and alanine (PubMed:10049700, PubMed:10574970, PubMed:11557028, PubMed:11564694, PubMed:12117417, PubMed:12225859, PubMed:15769744, PubMed:18262359, PubMed:25998567, PubMed:30867591, PubMed:9751058, PubMed:26305885). The heterodimer with SLC3A2 mediates the uptake of L-DOPA (By similarity). Functions as an amino acid exchanger (PubMed:11557028, PubMed:12117417, PubMed:12225859, PubMed:30867591). May play a role in the transport of L-DOPA across the blood-brain barrier (By similarity). May act as the major transporter of tyrosine in fibroblasts (Probable). May mediate blood-to-retina L-leucine transport across the inner blood-retinal barrier (By similarity). Can mediate the transport of thyroid hormones diiodothyronine (T2), triiodothyronine (T3) and thyroxine (T4) across the cell membrane (PubMed:11564694, PubMed:26305885). When associated with LAPTM4B, the heterodimer formed by SLC3A2 and SLC7A5 is recruited to lysosomes to promote leucine uptake into these organelles, and thereby mediates mTORC1 activation (PubMed:25998567). Involved in the uptake of toxic methylmercury (MeHg) when administered as the L-cysteine or D,L-homocysteine complexes (PubMed:12117417). Involved in the cellular activity of small molecular weight nitrosothiols, via the stereoselective transport of L-nitrosocysteine (L-CNSO) across the membrane (PubMed:15769744). Mediates the uptake of 3-iodo-L-tyrosine (MIT) (PubMed:26305885). Mediates the uptake of 3,5-diiodo-L-tyrosine (DIT) (PubMed:38661522)
Protein Sequence
10
MAGAGPKRRA
20
LAAPAAEEKE
30
EAREKMLAAK
40
SADGSAPAGE
50
GEGVTLQRNI
60
TLLNGVAIIV
70
GTIIGSGIFV
80
TPTGVLKEAG
90
SPGLALVVWA
100
ACGVFSIVGA
110
LCYAELGTTI
120
SKSGGDYAYM
130
LEVYGSLPAF
140
LKLWIELLII
150
RPSSQYIVAL
160
VFATYLLKPL
170
FPTCPVPEEA
180
AKLVACLCVL
190
LLTAVNCYSV
200
KAATRVQDAF
210
AAAKLLALAL
220
IILLGFVQIG
230
KGDVSNLDPN
240
FSFEGTKLDV
250
GNIVLALYSG
260
LFAYGGWNYL
270
NFVTEEMINP
280
YRNLPLAIII
290
SLPIVTLVYV
300
LTNLAYFTTL
310
STEQMLSSEA
320
VAVDFGNYHL
330
GVMSWIIPVF
340
VGLSCFGSVN
350
GSLFTSSRLF
360
FVGSREGHLP
370
SILSMIHPQL
380
LTPVPSLVFT
390
CVMTLLYAFS
400
KDIFSVINFF
410
SFFNWLCVAL
420
AIIGMIWLRH
430
RKPELERPIK
440
VNLALPVFFI
450
LACLFLIAVS
460
FWKTPVECGI
470
GFTIILSGLP
480
VYFFGVWWKN
490
KPKWLLQGIF
500
STTVLCQKLM
QVVPQET
Gene Ontology
| Classification |
GO ID |
Description |
| Cellular Component |
GO:1990184 |
amino acid transport complex |
| Cellular Component |
GO:0016324 |
apical plasma membrane |
| Cellular Component |
GO:0009925 |
basal plasma membrane |
| Cellular Component |
GO:0016323 |
basolateral plasma membrane |
| Cellular Component |
GO:0005829 |
cytosol |
| Cellular Component |
GO:0098591 |
external side of apical plasma membrane |
| Cellular Component |
GO:0070062 |
extracellular exosome |
| Cellular Component |
GO:0005765 |
lysosomal membrane |
| Cellular Component |
GO:0016020 |
membrane |
| Cellular Component |
GO:0031528 |
microvillus membrane |
| Cellular Component |
GO:0005886 |
plasma membrane |
| Molecular Function |
GO:0015171 |
amino acid transmembrane transporter activity |
| Molecular Function |
GO:0015297 |
antiporter activity |
| Molecular Function |
GO:0015173 |
aromatic amino acid transmembrane transporter activity |
| Molecular Function |
GO:0015179 |
L-amino acid transmembrane transporter activity |
| Molecular Function |
GO:0015190 |
L-leucine transmembrane transporter activity |
| Molecular Function |
GO:0015196 |
L-tryptophan transmembrane transporter activity |
| Molecular Function |
GO:0015175 |
neutral L-amino acid transmembrane transporter activity |
| Molecular Function |
GO:0042605 |
peptide antigen binding |
| Molecular Function |
GO:0015349 |
thyroid hormone transmembrane transporter activity |
| Biological Process |
GO:0032328 |
alanine transport |
| Biological Process |
GO:0089718 |
amino acid import across plasma membrane |
| Biological Process |
GO:0003333 |
amino acid transmembrane transport |
| Biological Process |
GO:0042149 |
cellular response to glucose starvation |
| Biological Process |
GO:1903577 |
cellular response to L-arginine |
| Biological Process |
GO:0071222 |
cellular response to lipopolysaccharide |
| Biological Process |
GO:0015818 |
isoleucine transport |
| Biological Process |
GO:1902024 |
L-histidine transport |
| Biological Process |
GO:1903801 |
L-leucine import across plasma membrane |
| Biological Process |
GO:0015820 |
L-leucine transport |
| Biological Process |
GO:1904556 |
L-tryptophan transmembrane transport |
| Biological Process |
GO:0097421 |
liver regeneration |
| Biological Process |
GO:0015821 |
methionine transport |
| Biological Process |
GO:0010507 |
negative regulation of autophagy |
| Biological Process |
GO:0010629 |
negative regulation of gene expression |
| Biological Process |
GO:1905460 |
negative regulation of vascular associated smooth muscle cell apoptotic process |
| Biological Process |
GO:0015804 |
neutral amino acid transport |
| Biological Process |
GO:0015823 |
phenylalanine transport |
| Biological Process |
GO:0002720 |
positive regulation of cytokine production involved in immune response |
| Biological Process |
GO:0060252 |
positive regulation of glial cell proliferation |
| Biological Process |
GO:0032740 |
positive regulation of interleukin-17 production |
| Biological Process |
GO:0032753 |
positive regulation of interleukin-4 production |
| Biological Process |
GO:1905534 |
positive regulation of L-leucine import across plasma membrane |
| Biological Process |
GO:0032729 |
positive regulation of type II interferon production |
| Biological Process |
GO:0015824 |
proline transport |
| Biological Process |
GO:0055093 |
response to hyperoxia |
| Biological Process |
GO:0014850 |
response to muscle activity |
| Biological Process |
GO:0070327 |
thyroid hormone transport |
| Biological Process |
GO:0150104 |
transport across blood-brain barrier |
| Biological Process |
GO:0015827 |
tryptophan transport |
| Biological Process |
GO:0015828 |
tyrosine transport |
| Biological Process |
GO:0015829 |
valine transport |
| Biological Process |
GO:0042908 |
xenobiotic transport |
Reference
[1] Cheng Z, Huang H, Li M, Chen Y. Proteomic analysis identifies PFKP lactylation in SW480 colon cancer cells.. iScience 27(1):108645. 2024 Jan 19. PMID: 38155775.