Search Results

Overview

Uniprot IDQ01813
Protein NameATP-dependent 6-phosphofructokinase, platelet type
Gene NamePFKP
OrganismHomo sapiens

Kla Sites from experimental identification

Position Flanking peptide
10 ADDSRAPKGSLRKFL
109 RTREGRLKAACNLLQ
15 APKGSLRKFLEHLSG
289 PITSEKIKELVVTQL
395 AGNLNTYKRLAIKLP
688 FDRNFGTKISARAME
736 FQPVAELKKQTDFEH

Function

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis

Protein Sequence

10 MDADDSRAPK 20 GSLRKFLEHL 30 SGAGKAIGVL 40 TSGGDAQGMN 50 AAVRAVVRMG 60 IYVGAKVYFI 70 YEGYQGMVDG 80 GSNIAEADWE 90 SVSSILQVGG 100 TIIGSARCQA 110 FRTREGRLKA 120 ACNLLQRGIT 130 NLCVIGGDGS 140 LTGANLFRKE 150 WSGLLEELAR 160 NGQIDKEAVQ 170 KYAYLNVVGM 180 VGSIDNDFCG 190 TDMTIGTDSA 200 LHRIIEVVDA 210 IMTTAQSHQR 220 TFVLEVMGRH 230 CGYLALVSAL 240 ACGADWVFLP 250 ESPPEEGWEE 260 QMCVKLSENR 270 ARKKRLNIII 280 VAEGAIDTQN 290 KPITSEKIKE 300 LVVTQLGYDT 310 RVTILGHVQR 320 GGTPSAFDRI 330 LASRMGVEAV 340 IALLEATPDT 350 PACVVSLNGN 360 HAVRLPLMEC 370 VQMTQDVQKA 380 MDERRFQDAV 390 RLRGRSFAGN 400 LNTYKRLAIK 410 LPDDQIPKTN 420 CNVAVINVGA 430 PAAGMNAAVR 440 SAVRVGIADG 450 HRMLAIYDGF 460 DGFAKGQIKE 470 IGWTDVGGWT 480 GQGGSILGTK 490 RVLPGKYLEE 500 IATQMRTHSI 510 NALLIIGGFE 520 AYLGLLELSA 530 AREKHEEFCV 540 PMVMVPATVS 550 NNVPGSDFSI 560 GADTALNTIT 570 DTCDRIKQSA 580 SGTKRRVFII 590 ETMGGYCGYL 600 ANMGGLAAGA 610 DAAYIFEEPF 620 DIRDLQSNVE 630 HLTEKMKTTI 640 QRGLVLRNES 650 CSENYTTDFI 660 YQLYSEEGKG 670 VFDCRKNVLG 680 HMQQGGAPSP 690 FDRNFGTKIS 700 ARAMEWITAK 710 LKEARGRGKK 720 FTTDDSICVL 730 GISKRNVIFQ 740 PVAELKKQTD 750 FEHRIPKEQW 760 WLKLRPLMKI 770 LAKYKASYDV 780 SDSGQLEHVQ PWSV

Gene Ontology

Classification GO ID Description
Cellular Component GO:0005945 6-phosphofructokinase complex
Cellular Component GO:0005737 cytoplasm
Cellular Component GO:0005829 cytosol
Cellular Component GO:0070062 extracellular exosome
Cellular Component GO:0016020 membrane
Cellular Component GO:0005634 nucleus
Molecular Function GO:0003872 6-phosphofructokinase activity
Molecular Function GO:0005524 ATP binding
Molecular Function GO:0045296 cadherin binding
Molecular Function GO:0070095 fructose-6-phosphate binding
Molecular Function GO:0042802 identical protein binding
Molecular Function GO:0046872 metal ion binding
Molecular Function GO:0044877 protein-containing complex binding
Biological Process GO:0061621 canonical glycolysis
Biological Process GO:0030388 fructose 1,6-bisphosphate metabolic process
Biological Process GO:0006002 fructose 6-phosphate metabolic process

Reference

[1] Yang D, Yin J, Shan L, Yi X, Zhang W et al.. Identification of lysine-lactylated substrates in gastric cancer cells.. iScience 25(7):104630. 2022 Jul 15. PMID: 35800753.

[2] Yang Z, Yan C, Ma J, Peng P, Ren X et al.. Lactylome analysis suggests lactylation-dependent mechanisms of metabolic adaptation in hepatocellular carcinoma.. Nat Metab 5(1):61-79. 2023 Jan. PMID: 36593272.

[3] Hong H, Chen X, Wang H, Gu X, Yuan Y et al.. Global profiling of protein lysine lactylation and potential target modified protein analysis in hepatocellular carcinoma.. Proteomics 23(9):e2200432. 2023 May. PMID: 36625413.

[4] Lin Y, Chen M, Wang D, Yu Y, Chen R et al.. Multi-Proteomic Analysis Reveals the Effect of Protein Lactylation on Matrix and Cholesterol Metabolism in Tendinopathy.. J Proteome Res 22(6):1712-1722. 2023 Jun 2. PMID: 37159428.

[5] Cheng Z, Huang H, Li M, Chen Y. Proteomic analysis identifies PFKP lactylation in SW480 colon cancer cells.. iScience 27(1):108645. 2024 Jan 19. PMID: 38155775.

[6] He C, Zhang J, Bai X, Lu C, Zhang K. Lysine lactylation-based insight to understanding the characterization of cervical cancer.. Biochim Biophys Acta Mol Basis Dis 1870(7):167356. 2024 Oct. PMID: 39025375.

[7] Bao Q, Wan N, He Z, Cao J, Yuan W et al.. Subcellular Proteomic Mapping of Lysine Lactylation.. J Am Soc Mass Spectrom 35(12):3221-3232. 2024 Dec 4. PMID: 39569522.

[8] Shi CM, Wang QC, Li XL, Yang YH, Tang XY et al.. Global Profiling of Protein Lactylation in Human Hippocampi.. Proteomics Clin Appl 19(2):e202400061. 2025 Mar. PMID: 39610256.

[9] He J, Lai T, Zhou Z, Yang H, Lei Z et al.. Multiomics profiling reveals the involvement of protein lactylation in nonhomologous end joining pathway conferring radioresistance in lung adenocarcinoma cell.. Sci Rep 15(1):24651. 2025 Jul 9. PMID: 40634431.

[10] Yan M, Tu H, Tang S, Gai Z, Shi Q et al.. Lactylated Proteomic Analysis Reveals Functional Implications of Lysine Lactylation In Asthenozoospermia.. Mol Cell Proteomics 24(12):101439. 2025 Dec. PMID: 41192556.

[11] Wu Q, Li Z, Gong T, Zheng X, Zhou X et al.. Porphyromonas gingivalis infection induces lysine lactylation reprogramming in human umbilical vein endothelial cells.. Front Cell Infect Microbiol 16:1706727. 2026. PMID: 41696360.