Search Results

Overview

Uniprot ID	Q01986
Protein Name	Dual specificity mitogen-activated protein kinase kinase 1
Gene Name	Map2k1
Organism	Rattus norvegicus

Kla Sites from experimental identification

Position	Flanking peptide
104	KLIHLEIKPAIRNQI
36	NLEALQKKLEELELD
57	LEAFLTQKQKVGELK
88	VVFKVSHKPSGLVMA
97	SGLVMARKLIHLEIK

Function

Dual specificity protein kinase which acts as an essential component of the MAP kinase signal transduction pathway. Binding of extracellular ligands such as growth factors, cytokines and hormones to their cell-surface receptors activates RAS and this initiates RAF1 activation. RAF1 then further activates the dual-specificity protein kinases MAP2K1/MEK1 and MAP2K2/MEK2. Both MAP2K1/MEK1 and MAP2K2/MEK2 function specifically in the MAPK/ERK cascade, and catalyze the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in the extracellular signal-regulated kinases MAPK3/ERK1 and MAPK1/ERK2, leading to their activation and further transduction of the signal within the MAPK/ERK cascade. Activates BRAF in a KSR1 or KSR2-dependent manner; by binding to KSR1 or KSR2 releases the inhibitory intramolecular interaction between KSR1 or KSR2 protein kinase and N-terminal domains which promotes KSR1 or KSR2-BRAF dimerization and BRAF activation (By similarity). Depending on the cellular context, this pathway mediates diverse biological functions such as cell growth, adhesion, survival and differentiation, predominantly through the regulation of transcription, metabolism and cytoskeletal rearrangements. One target of the MAPK/ERK cascade is peroxisome proliferator-activated receptor gamma (PPARG), a nuclear receptor that promotes differentiation and apoptosis. MAP2K1/MEK1 has been shown to export PPARG from the nucleus. The MAPK/ERK cascade is also involved in the regulation of endosomal dynamics, including lysosome processing and endosome cycling through the perinuclear recycling compartment (PNRC), as well as in the fragmentation of the Golgi apparatus during mitosis

Protein Sequence

10 MPKKKPTPIQ 20 LNPAPDGSAV 30 NGTSSAETNL 40 EALQKKLEEL 50 ELDEQQRKRL 60 EAFLTQKQKV 70 GELKDDDFEK 80 ISELGAGNGG 90 VVFKVSHKPS 100 GLVMARKLIH 110 LEIKPAIRNQ 120 IIRELQVLHE 130 CNSPYIVGFY 140 GAFYSDGEIS 150 ICMEHMDGGS 160 LDQVLKKAGR 170 IPEQILGKVS 180 IAVIKGLTYL 190 REKHKIMHRD 200 VKPSNILVNS 210 RGEIKLCDFG 220 VSGQLIDSMA 230 NSFVGTRSYM 240 SPERLQGTHY 250 SVQSDIWSMG 260 LSLVEMAVGR 270 YPIPPPDAKE 280 LELLFGCQVE 290 GDAAETPPRP 300 RTPGRPLSSY 310 GMDSRPPMAI 320 FELLDYIVNE 330 PPPKLPSGVF 340 SLEFQDFVNK 350 CLIKNPAERA 360 DLKQLMVHAF 370 IKRSDAEEVD 380 FAGWLCSTIG 390 LNQPSTPTHA ASI

Gene Ontology

Classification	GO ID	Description
Cellular Component	GO:0030424	axon
Molecular Function	GO:0043539	protein serine/threonine kinase activator activity
Molecular Function	GO:0004674	protein serine/threonine kinase activity
Molecular Function	GO:0004712	protein serine/threonine/tyrosine kinase activity
Molecular Function	GO:0004713	protein tyrosine kinase activity
Molecular Function	GO:0044877	protein-containing complex binding
Molecular Function	GO:0097110	scaffold protein binding
Molecular Function	GO:0031267	small GTPase binding
Biological Process	GO:0060020	Bergmann glial cell differentiation
Biological Process	GO:0048870	cell motility
Biological Process	GO:0090398	cellular senescence
Biological Process	GO:0021697	cerebellar cortex formation
Biological Process	GO:0060502	epithelial cell proliferation involved in lung morphogenesis
Biological Process	GO:0038127	ERBB signaling pathway
Biological Process	GO:0038133	ERBB2-ERBB3 signaling pathway
Biological Process	GO:0070371	ERK1 and ERK2 cascade
Biological Process	GO:0060324	face development
Biological Process	GO:0048313	Golgi inheritance
Biological Process	GO:0007507	heart development
Biological Process	GO:0008286	insulin receptor signaling pathway
Biological Process	GO:0048009	insulin-like growth factor receptor signaling pathway
Biological Process	GO:0035556	intracellular signal transduction
Biological Process	GO:0030216	keratinocyte differentiation
Biological Process	GO:0060711	labyrinthine layer development
Biological Process	GO:0060425	lung morphogenesis
Biological Process	GO:0000165	MAPK cascade
Biological Process	GO:0032402	melanosome transport
Biological Process	GO:0042552	myelination
Biological Process	GO:0008285	negative regulation of cell population proliferation
Biological Process	GO:0010629	negative regulation of gene expression
Biological Process	GO:0034111	negative regulation of homotypic cell-cell adhesion
Biological Process	GO:1903298	negative regulation of hypoxia-induced intrinsic apoptotic signaling pathway
Biological Process	GO:0007528	neuromuscular junction development
Biological Process	GO:0030182	neuron differentiation
Biological Process	GO:0048812	neuron projection morphogenesis
Biological Process	GO:0060674	placenta blood vessel development
Biological Process	GO:2001171	positive regulation of ATP biosynthetic process
Biological Process	GO:0010508	positive regulation of autophagy
Biological Process	GO:0050772	positive regulation of axonogenesis
Biological Process	GO:0045597	positive regulation of cell differentiation
Biological Process	GO:0030335	positive regulation of cell migration
Biological Process	GO:0045893	positive regulation of DNA-templated transcription
Biological Process	GO:0070374	positive regulation of ERK1 and ERK2 cascade
Biological Process	GO:0010628	positive regulation of gene expression
Biological Process	GO:0043547	positive regulation of GTPase activity
Biological Process	GO:0043410	positive regulation of MAPK cascade
Biological Process	GO:0045933	positive regulation of muscle contraction
Biological Process	GO:0071902	positive regulation of protein serine/threonine kinase activity
Biological Process	GO:0046579	positive regulation of Ras protein signal transduction
Biological Process	GO:0032968	positive regulation of transcription elongation by RNA polymerase II
Biological Process	GO:0006468	protein phosphorylation
Biological Process	GO:0048679	regulation of axon regeneration
Biological Process	GO:2000641	regulation of early endosome to late endosome transport
Biological Process	GO:0070372	regulation of ERK1 and ERK2 cascade
Biological Process	GO:0090170	regulation of Golgi inheritance
Biological Process	GO:0098696	regulation of neurotransmitter receptor localization to postsynaptic specialization membrane
Biological Process	GO:0032872	regulation of stress-activated MAPK cascade
Biological Process	GO:0003056	regulation of vascular associated smooth muscle contraction
Biological Process	GO:0048678	response to axon injury
Biological Process	GO:0051384	response to glucocorticoid
Biological Process	GO:0006979	response to oxidative stress
Biological Process	GO:0014044	Schwann cell development
Biological Process	GO:0048538	thymus development
Biological Process	GO:0030878	thyroid gland development
Biological Process	GO:0060440	trachea formation
Biological Process	GO:0070328	triglyceride homeostasis
Biological Process	GO:0047496	vesicle transport along microtubule
Cellular Component	GO:0005938	cell cortex
Cellular Component	GO:0005813	centrosome
Cellular Component	GO:0036064	ciliary basal body
Cellular Component	GO:0005829	cytosol
Cellular Component	GO:0030425	dendrite
Cellular Component	GO:0032839	dendrite cytoplasm
Cellular Component	GO:0005769	early endosome
Cellular Component	GO:0005783	endoplasmic reticulum
Cellular Component	GO:0005925	focal adhesion
Cellular Component	GO:0098978	glutamatergic synapse
Cellular Component	GO:0005794	Golgi apparatus
Cellular Component	GO:0005770	late endosome
Cellular Component	GO:0005874	microtubule
Cellular Component	GO:0005739	mitochondrion
Cellular Component	GO:0005634	nucleus
Cellular Component	GO:0043204	perikaryon
Cellular Component	GO:0048471	perinuclear region of cytoplasm
Cellular Component	GO:0005886	plasma membrane
Cellular Component	GO:0014069	postsynaptic density
Molecular Function	GO:0005524	ATP binding
Molecular Function	GO:0004708	MAP kinase kinase activity
Molecular Function	GO:0005078	MAP-kinase scaffold activity
Molecular Function	GO:0031435	mitogen-activated protein kinase kinase kinase binding
Molecular Function	GO:0030295	protein kinase activator activity
Molecular Function	GO:0019901	protein kinase binding
Molecular Function	GO:0106310	protein serine kinase activity

Reference

[1] Chen Y, Sun W, Sun Z, Zhao H, Wu T et al.. Effect of electroacupuncture on hippocampal protein lactylation in a rat model of vascular dementia.. Front Neurol 16:1629474. 2025. PMID: 40963935.