Search Results

Overview

Uniprot IDQ02218
Protein Name2-oxoglutarate dehydrogenase complex component E1
Gene NameOGDH
OrganismHomo sapiens

Kla Sites from experimental identification

Position Flanking peptide
1000 APATGNKKTHLTELQ
488 EAVMYVCKVAAEWRS
561 EYEEEISKYDKICEE
564 EEISKYDKICEEAFA
852 QILLPFRKPLIIFTP
899 AQNPENVKRLLFCTG
970 QGYYDYVKPRLRTTI

Function

2-oxoglutarate dehydrogenase (E1o) component of the 2-oxoglutarate dehydrogenase complex (OGDHC) (PubMed:24495017, PubMed:25210035, PubMed:28435050). Participates in the first step, rate limiting for the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2) catalyzed by the whole OGDHC (PubMed:24495017, PubMed:25210035, PubMed:28435050). Catalyzes the irreversible decarboxylation of 2-oxoglutarate (alpha-ketoglutarate) via the thiamine diphosphate (ThDP) cofactor and subsequent transfer of the decarboxylated acyl intermediate on an oxidized dihydrolipoyl group that is covalently amidated to the E2 enzyme (dihydrolipoyllysine-residue succinyltransferase or DLST) (PubMed:24495017, PubMed:25210035, PubMed:28435050, PubMed:35272141). Plays a key role in the Krebs (citric acid) cycle, which is a common pathway for oxidation of fuel molecules, including carbohydrates, fatty acids, and amino acids (PubMed:25210035). Can catalyze the decarboxylation of 2-oxoadipate in vitro, but at a much lower rate than 2-oxoglutarate (PubMed:28435050). Can also convert 2-keto-4-hydroxyglutarate (KHG) and CoA into malyl-CoA (By similarity). Mainly active in the mitochondrion (PubMed:29211711). A fraction of the 2-oxoglutarate dehydrogenase complex also localizes in the nucleus and is required for lysine succinylation of histones: associates with KAT2A on chromatin and provides succinyl-CoA to histone succinyltransferase KAT2A (PubMed:29211711)

Protein Sequence

10 MFHLRTCAAK 20 LRPLTASQTV 30 KTFSQNRPAA 40 ARTFQQIRCY 50 SAPVAAEPFL 60 SGTSSNYVEE 70 MYCAWLENPK 80 SVHKSWDIFF 90 RNTNAGAPPG 100 TAYQSPLPLS 110 RGSLAAVAHA 120 QSLVEAQPNV 130 DKLVEDHLAV 140 QSLIRAYQIR 150 GHHVAQLDPL 160 GILDADLDSS 170 VPADIISSTD 180 KLGFYGLDES 190 DLDKVFHLPT 200 TTFIGGQESA 210 LPLREIIRRL 220 EMAYCQHIGV 230 EFMFINDLEQ 240 CQWIRQKFET 250 PGIMQFTNEE 260 KRTLLARLVR 270 STRFEEFLQR 280 KWSSEKRFGL 290 EGCEVLIPAL 300 KTIIDKSSEN 310 GVDYVIMGMP 320 HRGRLNVLAN 330 VIRKELEQIF 340 CQFDSKLEAA 350 DEGSGDVKYH 360 LGMYHRRINR 370 VTDRNITLSL 380 VANPSHLEAA 390 DPVVMGKTKA 400 EQFYCGDTEG 410 KKVMSILLHG 420 DAAFAGQGIV 430 YETFHLSDLP 440 SYTTHGTVHV 450 VVNNQIGFTT 460 DPRMARSSPY 470 PTDVARVVNA 480 PIFHVNSDDP 490 EAVMYVCKVA 500 AEWRSTFHKD 510 VVVDLVCYRR 520 NGHNEMDEPM 530 FTQPLMYKQI 540 RKQKPVLQKY 550 AELLVSQGVV 560 NQPEYEEEIS 570 KYDKICEEAF 580 ARSKDEKILH 590 IKHWLDSPWP 600 GFFTLDGQPR 610 SMSCPSTGLT 620 EDILTHIGNV 630 ASSVPVENFT 640 IHGGLSRILK 650 TRGEMVKNRT 660 VDWALAEYMA 670 FGSLLKEGIH 680 IRLSGQDVER 690 GTFSHRHHVL 700 HDQNVDKRTC 710 IPMNHLWPNQ 720 APYTVCNSSL 730 SEYGVLGFEL 740 GFAMASPNAL 750 VLWEAQFGDF 760 HNTAQCIIDQ 770 FICPGQAKWV 780 RQNGIVLLLP 790 HGMEGMGPEH 800 SSARPERFLQ 810 MCNDDPDVLP 820 DLKEANFDIN 830 QLYDCNWVVV 840 NCSTPGNFFH 850 VLRRQILLPF 860 RKPLIIFTPK 870 SLLRHPEARS 880 SFDEMLPGTH 890 FQRVIPEDGP 900 AAQNPENVKR 910 LLFCTGKVYY 920 DLTRERKARD 930 MVGQVAITRI 940 EQLSPFPFDL 950 LLKEVQKYPN 960 AELAWCQEEH 970 KNQGYYDYVK 980 PRLRTTISRA 990 KPVWYAGRDP 1000 AAAPATGNKK 1010 THLTELQRLL 1020 DTAFDLDVFK NFS

Gene Ontology

Classification GO ID Description
Cellular Component GO:0005759 mitochondrial matrix
Cellular Component GO:0031966 mitochondrial membrane
Cellular Component GO:0005739 mitochondrion
Cellular Component GO:0005634 nucleus
Cellular Component GO:0045252 oxoglutarate dehydrogenase complex
Molecular Function GO:0016831 carboxy-lyase activity
Molecular Function GO:0031072 heat shock protein binding
Molecular Function GO:0046872 metal ion binding
Molecular Function GO:0004591 oxoglutarate dehydrogenase (succinyl-transferring) activity
Molecular Function GO:0051087 protein-folding chaperone binding
Molecular Function GO:0030976 thiamine pyrophosphate binding
Biological Process GO:0120551 2-oxoglutarate decarboxylation to succinyl-CoA
Biological Process GO:0006103 2-oxoglutarate metabolic process
Biological Process GO:0021695 cerebellar cortex development
Biological Process GO:0006091 generation of precursor metabolites and energy
Biological Process GO:0006096 glycolytic process
Biological Process GO:0021766 hippocampus development
Biological Process GO:0061034 olfactory bulb mitral cell layer development
Biological Process GO:0021860 pyramidal neuron development
Biological Process GO:0021756 striatum development
Biological Process GO:0006104 succinyl-CoA metabolic process
Biological Process GO:0022028 tangential migration from the subventricular zone to the olfactory bulb
Biological Process GO:0021794 thalamus development
Biological Process GO:0006099 tricarboxylic acid cycle

Reference

[1] Yang Z, Yan C, Ma J, Peng P, Ren X et al.. Lactylome analysis suggests lactylation-dependent mechanisms of metabolic adaptation in hepatocellular carcinoma.. Nat Metab 5(1):61-79. 2023 Jan. PMID: 36593272.

[2] Shi CM, Wang QC, Li XL, Yang YH, Tang XY et al.. Global Profiling of Protein Lactylation in Human Hippocampi.. Proteomics Clin Appl 19(2):e202400061. 2025 Mar. PMID: 39610256.

[3] He J, Lai T, Zhou Z, Yang H, Lei Z et al.. Multiomics profiling reveals the involvement of protein lactylation in nonhomologous end joining pathway conferring radioresistance in lung adenocarcinoma cell.. Sci Rep 15(1):24651. 2025 Jul 9. PMID: 40634431.