Search Results

Overview

Uniprot IDQ03164
Protein NameHistone-lysine N-methyltransferase 2A
Gene NameKMT2A
OrganismHomo sapiens

Kla Sites from experimental identification

Position Flanking peptide
1028 KRVASLLKKAKAQLC
1748 DGGQPEIKKANSMVK
1755 KKANSMVKSFFIRQM
1822 EQPPLMKKIIPAPKP
1828 KKIIPAPKPKGPGEP
2347 SRELNVSKIGSFAEP
2403 DTEVKTLKLSGMSNR
2521 APRKRTVKVTLTPLK
2528 KVTLTPLKMENESQS
2613 PQEDGSFKRRYPRRS
2654 FYSSSTGKKRGKRSA
2655 YSSSTGKKRGKRSAE
2742 RKSSQIPKRNGKENG
289 KSKFKTGKLQIGRKG
3219 ATPSSGLKKRPISRL
928 VATSSSAKKATGRKK
929 ATSSSAKKATGRKKS

Function

Histone methyltransferase that plays an essential role in early development and hematopoiesis (PubMed:12453419, PubMed:15960975, PubMed:19187761, PubMed:19556245, PubMed:20677832, PubMed:21220120, PubMed:26886794). Catalytic subunit of the MLL1/MLL complex, a multiprotein complex that mediates both methylation of 'Lys-4' of histone H3 (H3K4me) complex and acetylation of 'Lys-16' of histone H4 (H4K16ac) (PubMed:12453419, PubMed:15960975, PubMed:19187761, PubMed:19556245, PubMed:20677832, PubMed:21220120, PubMed:24235145, PubMed:26886794). Catalyzes methyl group transfer from S-adenosyl-L-methionine to the epsilon-amino group of 'Lys-4' of histone H3 (H3K4) via a non-processive mechanism. Part of chromatin remodeling machinery predominantly forms H3K4me1 and H3K4me2 methylation marks at active chromatin sites where transcription and DNA repair take place (PubMed:12453419, PubMed:15960975, PubMed:19187761, PubMed:19556245, PubMed:20677832, PubMed:21220120, PubMed:25561738, PubMed:26886794). Has weak methyltransferase activity by itself, and requires other component of the MLL1/MLL complex to obtain full methyltransferase activity (PubMed:19187761, PubMed:26886794). Has no activity toward histone H3 phosphorylated on 'Thr-3', less activity toward H3 dimethylated on 'Arg-8' or 'Lys-9', while it has higher activity toward H3 acetylated on 'Lys-9' (PubMed:19187761). Binds to unmethylated CpG elements in the promoter of target genes and helps maintain them in the nonmethylated state (PubMed:20010842). Required for transcriptional activation of HOXA9 (PubMed:12453419, PubMed:20010842, PubMed:20677832). Promotes PPP1R15A-induced apoptosis (PubMed:10490642). Plays a critical role in the control of circadian gene expression and is essential for the transcriptional activation mediated by the CLOCK-BMAL1 heterodimer (By similarity). Establishes a permissive chromatin state for circadian transcription by mediating a rhythmic methylation of 'Lys-4' of histone H3 (H3K4me) and this histone modification directs the circadian acetylation at H3K9 and H3K14 allowing the recruitment of CLOCK-BMAL1 to chromatin (By similarity). Also has auto-methylation activity on Cys-3882 in absence of histone H3 substrate (PubMed:24235145)

Protein Sequence

10 MAHSCRWRFP 20 ARPGTTGGGG 30 GGGRRGLGGA 40 PRQRVPALLL 50 PPGPPVGGGG 60 PGAPPSPPAV 70 AAAAAAAGSS 80 GAGVPGGAAA 90 ASAASSSSAS 100 SSSSSSSSAS 110 SGPALLRVGP 120 GFDAALQVSA 130 AIGTNLRRFR 140 AVFGESGGGG 150 GSGEDEQFLG 160 FGSDEEVRVR 170 SPTRSPSVKT 180 SPRKPRGRPR 190 SGSDRNSAIL 200 SDPSVFSPLN 210 KSETKSGDKI 220 KKKDSKSIEK 230 KRGRPPTFPG 240 VKIKITHGKD 250 ISELPKGNKE 260 DSLKKIKRTP 270 SATFQQATKI 280 KKLRAGKLSP 290 LKSKFKTGKL 300 QIGRKGVQIV 310 RRRGRPPSTE 320 RIKTPSGLLI 330 NSELEKPQKV 340 RKDKEGTPPL 350 TKEDKTVVRQ 360 SPRRIKPVRI 370 IPSSKRTDAT 380 IAKQLLQRAK 390 KGAQKKIEKE 400 AAQLQGRKVK 410 TQVKNIRQFI 420 MPVVSAISSR 430 IIKTPRRFIE 440 DEDYDPPIKI 450 ARLESTPNSR 460 FSAPSCGSSE 470 KSSAASQHSS 480 QMSSDSSRSS 490 SPSVDTSTDS 500 QASEEIQVLP 510 EERSDTPEVH 520 PPLPISQSPE 530 NESNDRRSRR 540 YSVSERSFGS 550 RTTKKLSTLQ 560 SAPQQQTSSS 570 PPPPLLTPPP 580 PLQPASSISD 590 HTPWLMPPTI 600 PLASPFLPAS 610 TAPMQGKRKS 620 ILREPTFRWT 630 SLKHSRSEPQ 640 YFSSAKYAKE 650 GLIRKPIFDN 660 FRPPPLTPED 670 VGFASGFSAS 680 GTAASARLFS 690 PLHSGTRFDM 700 HKRSPLLRAP 710 RFTPSEAHSR 720 IFESVTLPSN 730 RTSAGTSSSG 740 VSNRKRKRKV 750 FSPIRSEPRS 760 PSHSMRTRSG 770 RLSSSELSPL 780 TPPSSVSSSL 790 SISVSPLATS 800 ALNPTFTFPS 810 HSLTQSGESA 820 EKNQRPRKQT 830 SAPAEPFSSS 840 SPTPLFPWFT 850 PGSQTERGRN 860 KDKAPEELSK 870 DRDADKSVEK 880 DKSRERDRER 890 EKENKRESRK 900 EKRKKGSEIQ 910 SSSALYPVGR 920 VSKEKVVGED 930 VATSSSAKKA 940 TGRKKSSSHD 950 SGTDITSVTL 960 GDTTAVKTKI 970 LIKKGRGNLE 980 KTNLDLGPTA 990 PSLEKEKTLC 1000 LSTPSSSTVK 1010 HSTSSIGSML 1020 AQADKLPMTD 1030 KRVASLLKKA 1040 KAQLCKIEKS 1050 KSLKQTDQPK 1060 AQGQESDSSE 1070 TSVRGPRIKH 1080 VCRRAAVALG 1090 RKRAVFPDDM 1100 PTLSALPWEE 1110 REKILSSMGN 1120 DDKSSIAGSE 1130 DAEPLAPPIK 1140 PIKPVTRNKA 1150 PQEPPVKKGR 1160 RSRRCGQCPG 1170 CQVPEDCGVC 1180 TNCLDKPKFG 1190 GRNIKKQCCK 1200 MRKCQNLQWM 1210 PSKAYLQKQA 1220 KAVKKKEKKS 1230 KTSEKKDSKE 1240 SSVVKNVVDS 1250 SQKPTPSARE 1260 DPAPKKSSSE 1270 PPPRKPVEEK 1280 SEEGNVSAPG 1290 PESKQATTPA 1300 SRKSSKQVSQ 1310 PALVIPPQPP 1320 TTGPPRKEVP 1330 KTTPSEPKKK 1340 QPPPPESGPE 1350 QSKQKKVAPR 1360 PSIPVKQKPK 1370 EKEKPPPVNK 1380 QENAGTLNIL 1390 STLSNGNSSK 1400 QKIPADGVHR 1410 IRVDFKEDCE 1420 AENVWEMGGL 1430 GILTSVPITP 1440 RVVCFLCASS 1450 GHVEFVYCQV 1460 CCEPFHKFCL 1470 EENERPLEDQ 1480 LENWCCRRCK 1490 FCHVCGRQHQ 1500 ATKQLLECNK 1510 CRNSYHPECL 1520 GPNYPTKPTK 1530 KKKVWICTKC 1540 VRCKSCGSTT 1550 PGKGWDAQWS 1560 HDFSLCHDCA 1570 KLFAKGNFCP 1580 LCDKCYDDDD 1590 YESKMMQCGK 1600 CDRWVHSKCE 1610 NLSDEMYEIL 1620 SNLPESVAYT 1630 CVNCTERHPA 1640 EWRLALEKEL 1650 QISLKQVLTA 1660 LLNSRTTSHL 1670 LRYRQAAKPP 1680 DLNPETEESI 1690 PSRSSPEGPD 1700 PPVLTEVSKQ 1710 DDQQPLDLEG 1720 VKRKMDQGNY 1730 TSVLEFSDDI 1740 VKIIQAAINS 1750 DGGQPEIKKA 1760 NSMVKSFFIR 1770 QMERVFPWFS 1780 VKKSRFWEPN 1790 KVSSNSGMLP 1800 NAVLPPSLDH 1810 NYAQWQEREE 1820 NSHTEQPPLM 1830 KKIIPAPKPK 1840 GPGEPDSPTP 1850 LHPPTPPILS 1860 TDRSREDSPE 1870 LNPPPGIEDN 1880 RQCALCLTYG 1890 DDSANDAGRL 1900 LYIGQNEWTH 1910 VNCALWSAEV 1920 FEDDDGSLKN 1930 VHMAVIRGKQ 1940 LRCEFCQKPG 1950 ATVGCCLTSC 1960 TSNYHFMCSR 1970 AKNCVFLDDK 1980 KVYCQRHRDL 1990 IKGEVVPENG 2000 FEVFRRVFVD 2010 FEGISLRRKF 2020 LNGLEPENIH 2030 MMIGSMTIDC 2040 LGILNDLSDC 2050 EDKLFPIGYQ 2060 CSRVYWSTTD 2070 ARKRCVYTCK 2080 IVECRPPVVE 2090 PDINSTVEHD 2100 ENRTIAHSPT 2110 SFTESSSKES 2120 QNTAEIISPP 2130 SPDRPPHSQT 2140 SGSCYYHVIS 2150 KVPRIRTPSY 2160 SPTQRSPGCR 2170 PLPSAGSPTP 2180 TTHEIVTVGD 2190 PLLSSGLRSI 2200 GSRRHSTSSL 2210 SPQRSKLRIM 2220 SPMRTGNTYS 2230 RNNVSSVSTT 2240 GTATDLESSA 2250 KVVDHVLGPL 2260 NSSTSLGQNT 2270 STSSNLQRTV 2280 VTVGNKNSHL 2290 DGSSSSEMKQ 2300 SSASDLVSKS 2310 SSLKGEKTKV 2320 LSSKSSEGSA 2330 HNVAYPGIPK 2340 LAPQVHNTTS 2350 RELNVSKIGS 2360 FAEPSSVSFS 2370 SKEALSFPHL 2380 HLRGQRNDRD 2390 QHTDSTQSAN 2400 SSPDEDTEVK 2410 TLKLSGMSNR 2420 SSIINEHMGS 2430 SSRDRRQKGK 2440 KSCKETFKEK 2450 HSSKSFLEPG 2460 QVTTGEEGNL 2470 KPEFMDEVLT 2480 PEYMGQRPCN 2490 NVSSDKIGDK 2500 GLSMPGVPKA 2510 PPMQVEGSAK 2520 ELQAPRKRTV 2530 KVTLTPLKME 2540 NESQSKNALK 2550 ESSPASPLQI 2560 ESTSPTEPIS 2570 ASENPGDGPV 2580 AQPSPNNTSC 2590 QDSQSNNYQN 2600 LPVQDRNLML 2610 PDGPKPQEDG 2620 SFKRRYPRRS 2630 ARARSNMFFG 2640 LTPLYGVRSY 2650 GEEDIPFYSS 2660 STGKKRGKRS 2670 AEGQVDGADD 2680 LSTSDEDDLY 2690 YYNFTRTVIS 2700 SGGEERLASH 2710 NLFREEEQCD 2720 LPKISQLDGV 2730 DDGTESDTSV 2740 TATTRKSSQI 2750 PKRNGKENGT 2760 ENLKIDRPED 2770 AGEKEHVTKS 2780 SVGHKNEPKM 2790 DNCHSVSRVK 2800 TQGQDSLEAQ 2810 LSSLESSRRV 2820 HTSTPSDKNL 2830 LDTYNTELLK 2840 SDSDNNNSDD 2850 CGNILPSDIM 2860 DFVLKNTPSM 2870 QALGESPESS 2880 SSELLNLGEG 2890 LGLDSNREKD 2900 MGLFEVFSQQ 2910 LPTTEPVDSS 2920 VSSSISAEEQ 2930 FELPLELPSD 2940 LSVLTTRSPT 2950 VPSQNPSRLA 2960 VISDSGEKRV 2970 TITEKSVASS 2980 ESDPALLSPG 2990 VDPTPEGHMT 3000 PDHFIQGHMD 3010 ADHISSPPCG 3020 SVEQGHGNNQ 3030 DLTRNSSTPG 3040 LQVPVSPTVP 3050 IQNQKYVPNS 3060 TDSPGPSQIS 3070 NAAVQTTPPH 3080 LKPATEKLIV 3090 VNQNMQPLYV 3100 LQTLPNGVTQ 3110 KIQLTSSVSS 3120 TPSVMETNTS 3130 VLGPMGGGLT 3140 LTTGLNPSLP 3150 TSQSLFPSAS 3160 KGLLPMSHHQ 3170 HLHSFPAATQ 3180 SSFPPNISNP 3190 PSGLLIGVQP 3200 PPDPQLLVSE 3210 SSQRTDLSTT 3220 VATPSSGLKK 3230 RPISRLQTRK 3240 NKKLAPSSTP 3250 SNIAPSDVVS 3260 NMTLINFTPS 3270 QLPNHPSLLD 3280 LGSLNTSSHR 3290 TVPNIIKRSK 3300 SSIMYFEPAP 3310 LLPQSVGGTA 3320 ATAAGTSTIS 3330 QDTSHLTSGS 3340 VSGLASSSSV 3350 LNVVSMQTTT 3360 TPTSSASVPG 3370 HVTLTNPRLL 3380 GTPDIGSISN 3390 LLIKASQQSL 3400 GIQDQPVALP 3410 PSSGMFPQLG 3420 TSQTPSTAAI 3430 TAASSICVLP 3440 STQTTGITAA 3450 SPSGEADEHY 3460 QLQHVNQLLA 3470 SKTGIHSSQR 3480 DLDSASGPQV 3490 SNFTQTVDAP 3500 NSMGLEQNKA 3510 LSSAVQASPT 3520 SPGGSPSSPS 3530 SGQRSASPSV 3540 PGPTKPKPKT 3550 KRFQLPLDKG 3560 NGKKHKVSHL 3570 RTSSSEAHIP 3580 DQETTSLTSG 3590 TGTPGAEAEQ 3600 QDTASVEQSS 3610 QKECGQPAGQ 3620 VAVLPEVQVT 3630 QNPANEQESA 3640 EPKTVEEEES 3650 NFSSPLMLWL 3660 QQEQKRKESI 3670 TEKKPKKGLV 3680 FEISSDDGFQ 3690 ICAESIEDAW 3700 KSLTDKVQEA 3710 RSNARLKQLS 3720 FAGVNGLRML 3730 GILHDAVVFL 3740 IEQLSGAKHC 3750 RNYKFRFHKP 3760 EEANEPPLNP 3770 HGSARAEVHL 3780 RKSAFDMFNF 3790 LASKHRQPPE 3800 YNPNDEEEEE 3810 VQLKSARRAT 3820 SMDLPMPMRF 3830 RHLKKTSKEA 3840 VGVYRSPIHG 3850 RGLFCKRNID 3860 AGEMVIEYAG 3870 NVIRSIQTDK 3880 REKYYDSKGI 3890 GCYMFRIDDS 3900 EVVDATMHGN 3910 AARFINHSCE 3920 PNCYSRVINI 3930 DGQKHIVIFA 3940 MRKIYRGEEL 3950 TYDYKFPIED 3960 ASNKLPCNCG AKKCRKFLN

Gene Ontology

Classification GO ID Description
Cellular Component GO:0005829 cytosol
Cellular Component GO:0035097 histone methyltransferase complex
Cellular Component GO:0071339 MLL1 complex
Cellular Component GO:0005654 nucleoplasm
Cellular Component GO:0005634 nucleus
Molecular Function GO:0003682 chromatin binding
Molecular Function GO:0042800 histone H3K4 methyltransferase activity
Molecular Function GO:0140945 histone H3K4 monomethyltransferase activity
Molecular Function GO:0140999 histone H3K4 trimethyltransferase activity
Molecular Function GO:0042802 identical protein binding
Molecular Function GO:0003680 minor groove of adenine-thymine-rich DNA binding
Molecular Function GO:0042803 protein homodimerization activity
Molecular Function GO:0106363 protein-cysteine methyltransferase activity
Molecular Function GO:0045322 unmethylated CpG binding
Molecular Function GO:0008270 zinc ion binding
Biological Process GO:0006915 apoptotic process
Biological Process GO:0071560 cellular response to transforming growth factor beta stimulus
Biological Process GO:0032922 circadian regulation of gene expression
Biological Process GO:0060216 definitive hemopoiesis
Biological Process GO:0035162 embryonic hemopoiesis
Biological Process GO:0051899 membrane depolarization
Biological Process GO:0032259 methylation
Biological Process GO:0090310 negative regulation of DNA methylation-dependent heterochromatin formation
Biological Process GO:0045893 positive regulation of DNA-templated transcription
Biological Process GO:0045944 positive regulation of transcription by RNA polymerase II
Biological Process GO:0065003 protein-containing complex assembly
Biological Process GO:0035864 response to potassium ion
Biological Process GO:0045064 T-helper 2 cell differentiation
Biological Process GO:0045815 transcription initiation-coupled chromatin remodeling

Reference

[1] Yang D, Yin J, Shan L, Yi X, Zhang W et al.. Identification of lysine-lactylated substrates in gastric cancer cells.. iScience 25(7):104630. 2022 Jul 15. PMID: 35800753.

[2] Yang Z, Yan C, Ma J, Peng P, Ren X et al.. Lactylome analysis suggests lactylation-dependent mechanisms of metabolic adaptation in hepatocellular carcinoma.. Nat Metab 5(1):61-79. 2023 Jan. PMID: 36593272.

[3] He C, Zhang J, Bai X, Lu C, Zhang K. Lysine lactylation-based insight to understanding the characterization of cervical cancer.. Biochim Biophys Acta Mol Basis Dis 1870(7):167356. 2024 Oct. PMID: 39025375.

[4] Wu Q, Li Z, Gong T, Zheng X, Zhou X et al.. Porphyromonas gingivalis infection induces lysine lactylation reprogramming in human umbilical vein endothelial cells.. Front Cell Infect Microbiol 16:1706727. 2026. PMID: 41696360.