Search Results
Overview
| Uniprot ID | Q03344 |
|---|---|
| Protein Name | ATPase inhibitor, mitochondrial |
| Gene Name | Atp5if1 |
| Organism | Rattus norvegicus |
Kla Sites from experimental identification
| Position | Flanking peptide |
|---|---|
| 103 | KKKIKYLKNSEH*** |
| 49 | EAGGAFGKREKAEED |
| 52 | GAFGKREKAEEDRYF |
| 71 | REQLAALKKHHEDEI |
| 72 | EQLAALKKHHEDEID |
| 83 | DEIDHHSKEIERLQK |
| 90 | KEIERLQKQIERHKK |
Function
Endogenous F(1)F(o)-ATPase inhibitor limiting ATP depletion when the mitochondrial membrane potential falls below a threshold and the F(1)F(o)-ATP synthase starts hydrolyzing ATP to pump protons out of the mitochondrial matrix. Required to avoid the consumption of cellular ATP when the F(1)F(o)-ATP synthase enzyme acts as an ATP hydrolase (By similarity). Indirectly acts as a regulator of heme synthesis in erythroid tissues: regulates heme synthesis by modulating the mitochondrial pH and redox potential, allowing FECH to efficiently catalyze the incorporation of iron into protoporphyrin IX to produce heme (By similarity)
Protein Sequence
Gene Ontology
| Classification | GO ID | Description |
|---|---|---|
| Cellular Component | GO:0009986 | cell surface |
| Cellular Component | GO:0005737 | cytoplasm |
| Cellular Component | GO:0005739 | mitochondrion |
| Cellular Component | GO:0032991 | protein-containing complex |
| Molecular Function | GO:0043532 | angiostatin binding |
| Molecular Function | GO:0051117 | ATPase binding |
| Molecular Function | GO:0042030 | ATPase inhibitor activity |
| Molecular Function | GO:0005516 | calmodulin binding |
| Molecular Function | GO:0019899 | enzyme binding |
| Molecular Function | GO:0042802 | identical protein binding |
| Molecular Function | GO:0140260 | mitochondrial proton-transporting ATP synthase complex binding |
| Biological Process | GO:0030218 | erythrocyte differentiation |
| Biological Process | GO:0006783 | heme biosynthetic process |
| Biological Process | GO:0051882 | mitochondrial depolarization |
| Biological Process | GO:0010667 | negative regulation of cardiac muscle cell apoptotic process |
| Biological Process | GO:0001937 | negative regulation of endothelial cell proliferation |
| Biological Process | GO:0051346 | negative regulation of hydrolase activity |
| Biological Process | GO:1905707 | negative regulation of mitochondrial ATP synthesis coupled proton transport |
| Biological Process | GO:1901030 | positive regulation of mitochondrial outer membrane permeabilization involved in apoptotic signaling pathway |
| Biological Process | GO:1903052 | positive regulation of proteolysis involved in protein catabolic process |
| Biological Process | GO:1905091 | positive regulation of type 2 mitophagy |
| Biological Process | GO:0072593 | reactive oxygen species metabolic process |
| Biological Process | GO:1903578 | regulation of ATP metabolic process |
| Biological Process | GO:1903214 | regulation of protein targeting to mitochondrion |
| Biological Process | GO:0002931 | response to ischemia |
Reference
[1] Yao Y, Bade R, Li G, Zhang A, Zhao H et al.. Global-Scale Profiling of Differential Expressed Lysine-Lactylated Proteins in the Cerebral Endothelium of Cerebral Ischemia-Reperfusion Injury Rats.. Cell Mol Neurobiol 43(5):1989-2004. 2023 Jul. PMID: 36030297.
[2] Sheng L, Xu H, Wang Y, Ni J, Xiang T et al.. Systematic analysis of lysine lactylation in nucleus pulposus cells.. iScience 27(11):111157. 2024 Nov 15. PMID: 39524337.
[3] Chen Y, Sun W, Sun Z, Zhao H, Wu T et al.. Effect of electroacupuncture on hippocampal protein lactylation in a rat model of vascular dementia.. Front Neurol 16:1629474. 2025. PMID: 40963935.