Search Results

Overview

Uniprot IDQ03468
Protein NameDNA excision repair protein ERCC-6
Gene NameERCC6
OrganismHomo sapiens

Kla Sites from experimental identification

Position Flanking peptide
1052 GADHDVPKRKKFPAS
1054 DHDVPKRKKFPASNI
297 CNKRAARKAPAPVTP
323 KKARVLSKKEERLKK

Function

Essential factor involved in transcription-coupled nucleotide excision repair (TC-NER), a process during which RNA polymerase II-blocking lesions are rapidly removed from the transcribed strand of active genes (PubMed:16246722, PubMed:20541997, PubMed:22483866, PubMed:26620705, PubMed:32355176, PubMed:34526721, PubMed:38316879, PubMed:38600235, PubMed:38600236). Plays a central role in the initiation of the TC-NER process: specifically recognizes and binds RNA polymerase II stalled at a lesion, and mediates recruitment of ERCC8/CSA, initiating DNA damage excision by TFIIH recruitment (PubMed:32355176, PubMed:34526721, PubMed:38600235, PubMed:38600236). Upon DNA-binding, it locally modifies DNA conformation by wrapping the DNA around itself, thereby modifying the interface between stalled RNA polymerase II and DNA (PubMed:15548521). Acts as a chromatin remodeler at DSBs; DNA-dependent ATPase-dependent activity is essential for this function (PubMed:16246722, PubMed:9565609). Plays an important role in regulating the choice of the DNA double-strand breaks (DSBs) repair pathway and G2/M checkpoint activation; DNA-dependent ATPase activity is essential for this function (PubMed:25820262). Regulates the DNA repair pathway choice by inhibiting non-homologous end joining (NHEJ), thereby promoting the homologous recombination (HR)-mediated repair of DSBs during the S/G2 phases of the cell cycle (PubMed:25820262). Mediates the activation of the ATM- and CHEK2-dependent DNA damage responses thus preventing premature entry of cells into mitosis following the induction of DNA DSBs (PubMed:25820262). Remodels chromatin by evicting histones from chromatin flanking DSBs, limiting RIF1 accumulation at DSBs thereby promoting BRCA1-mediated HR (PubMed:29203878). Required for stable recruitment of ELOA and CUL5 to DNA damage sites (PubMed:28292928). Also involved in UV-induced translocation of ERCC8 to the nuclear matrix (PubMed:26620705). Essential for neuronal differentiation and neuritogenesis; regulates transcription and chromatin remodeling activities required during neurogenesis (PubMed:24874740)

Protein Sequence

10 MPNEGIPHSS 20 QTQEQDCLQS 30 QPVSNNEEMA 40 IKQESGGDGE 50 VEEYLSFRSV 60 GDGLSTSAVG 70 CASAAPRRGP 80 ALLHIDRHQI 90 QAVEPSAQAL 100 ELQGLGVDVY 110 DQDVLEQGVL 120 QQVDNAIHEA 130 SRASQLVDVE 140 KEYRSVLDDL 150 TSCTTSLRQI 160 NKIIEQLSPQ 170 AATSRDINRK 180 LDSVKRQKYN 190 KEQQLKKITA 200 KQKHLQAILG 210 GAEVKIELDH 220 ASLEEDAEPG 230 PSSLGSMLMP 240 VQETAWEELI 250 RTGQMTPFGT 260 QIPQKQEKKP 270 RKIMLNEASG 280 FEKYLADQAK 290 LSFERKKQGC 300 NKRAARKAPA 310 PVTPPAPVQN 320 KNKPNKKARV 330 LSKKEERLKK 340 HIKKLQKRAL 350 QFQGKVGLPK 360 ARRPWESDMR 370 PEAEGDSEGE 380 ESEYFPTEEE 390 EEEEDDEVEG 400 AEADLSGDGT 410 DYELKPLPKG 420 GKRQKKVPVQ 430 EIDDDFFPSS 440 GEEAEAASVG 450 EGGGGGRKVG 460 RYRDDGDEDY 470 YKQRLRRWNK 480 LRLQDKEKRL 490 KLEDDSEESD 500 AEFDEGFKVP 510 GFLFKKLFKY 520 QQTGVRWLWE 530 LHCQQAGGIL 540 GDEMGLGKTI 550 QIIAFLAGLS 560 YSKIRTRGSN 570 YRFEGLGPTV 580 IVCPTTVMHQ 590 WVKEFHTWWP 600 PFRVAILHET 610 GSYTHKKEKL 620 IRDVAHCHGI 630 LITSYSYIRL 640 MQDDISRYDW 650 HYVILDEGHK 660 IRNPNAAVTL 670 ACKQFRTPHR 680 IILSGSPMQN 690 NLRELWSLFD 700 FIFPGKLGTL 710 PVFMEQFSVP 720 ITMGGYSNAS 730 PVQVKTAYKC 740 ACVLRDTINP 750 YLLRRMKSDV 760 KMSLSLPDKN 770 EQVLFCRLTD 780 EQHKVYQNFV 790 DSKEVYRILN 800 GEMQIFSGLI 810 ALRKICNHPD 820 LFSGGPKNLK 830 GLPDDELEED 840 QFGYWKRSGK 850 MIVVESLLKI 860 WHKQGQRVLL 870 FSQSRQMLDI 880 LEVFLRAQKY 890 TYLKMDGTTT 900 IASRQPLITR 910 YNEDTSIFVF 920 LLTTRVGGLG 930 VNLTGANRVV 940 IYDPDWNPST 950 DTQARERAWR 960 IGQKKQVTVY 970 RLLTAGTIEE 980 KIYHRQIFKQ 990 FLTNRVLKDP 1000 KQRRFFKSND 1010 LYELFTLTSP 1020 DASQSTETSA 1030 IFAGTGSDVQ 1040 TPKCHLKRRI 1050 QPAFGADHDV 1060 PKRKKFPASN 1070 ISVNDATSSE 1080 EKSEAKGAEV 1090 NAVTSNRSDP 1100 LKDDPHMSSN 1110 VTSNDRLGEE 1120 TNAVSGPEEL 1130 SVISGNGECS 1140 NSSGTGKTSM 1150 PSGDESIDEK 1160 LGLSYKRERP 1170 SQAQTEAFWE 1180 NKQMENNFYK 1190 HKSKTKHHSV 1200 AEEETLEKHL 1210 RPKQKPKNSK 1220 HCRDAKFEGT 1230 RIPHLVKKRR 1240 YQKQDSENKS 1250 EAKEQSNDDY 1260 VLEKLFKKSV 1270 GVHSVMKHDA 1280 IMDGASPDYV 1290 LVEAEANRVA 1300 QDALKALRLS 1310 RQRCLGAVSG 1320 VPTWTGHRGI 1330 SGAPAGKKSR 1340 FGKKRNSNFS 1350 VQHPSSTSPT 1360 EKCQDGIMKK 1370 EGKDNVPEHF 1380 SGRAEDADSS 1390 SGPLASSSLL 1400 AKMRARNHLI 1410 LPERLESESG 1420 HLQEASALLP 1430 TTEHDDLLVE 1440 MRNFIAFQAH 1450 TDGQASTREI 1460 LQEFESKLSA 1470 SQSCVFRELL 1480 RNLCTFHRTS 1490 GGEGIWKLKP EYC

Gene Ontology

Classification GO ID Description
Cellular Component GO:0016604 nuclear body
Cellular Component GO:0005730 nucleolus
Cellular Component GO:0005654 nucleoplasm
Cellular Component GO:0005634 nucleus
Cellular Component GO:0090734 site of DNA damage
Cellular Component GO:0008023 transcription elongation factor complex
Molecular Function GO:0005524 ATP binding
Molecular Function GO:0016887 ATP hydrolysis activity
Molecular Function GO:0008094 ATP-dependent activity, acting on DNA
Molecular Function GO:0140658 ATP-dependent chromatin remodeler activity
Molecular Function GO:0140664 ATP-dependent DNA damage sensor activity
Molecular Function GO:0003682 chromatin binding
Molecular Function GO:0140463 chromatin-protein adaptor activity
Molecular Function GO:0003677 DNA binding
Molecular Function GO:0004386 helicase activity
Molecular Function GO:0030296 protein tyrosine kinase activator activity
Molecular Function GO:0070063 RNA polymerase binding
Biological Process GO:0006284 base-excision repair
Biological Process GO:0006338 chromatin remodeling
Biological Process GO:0000077 DNA damage checkpoint signaling
Biological Process GO:0042262 DNA protection
Biological Process GO:0006281 DNA repair
Biological Process GO:0097680 double-strand break repair via classical nonhomologous end joining
Biological Process GO:2001033 negative regulation of double-strand break repair via nonhomologous end joining
Biological Process GO:0022008 neurogenesis
Biological Process GO:0030182 neuron differentiation
Biological Process GO:0031175 neuron projection development
Biological Process GO:0045739 positive regulation of DNA repair
Biological Process GO:0032786 positive regulation of DNA-templated transcription, elongation
Biological Process GO:1905168 positive regulation of double-strand break repair via homologous recombination
Biological Process GO:0045943 positive regulation of transcription by RNA polymerase I
Biological Process GO:0045944 positive regulation of transcription by RNA polymerase II
Biological Process GO:0045945 positive regulation of transcription by RNA polymerase III
Biological Process GO:0071168 protein localization to chromatin
Biological Process GO:0032784 regulation of DNA-templated transcription elongation
Biological Process GO:0034243 regulation of transcription elongation by RNA polymerase II
Biological Process GO:0006979 response to oxidative stress
Biological Process GO:0000012 single strand break repair
Biological Process GO:0006366 transcription by RNA polymerase II
Biological Process GO:0006283 transcription-coupled nucleotide-excision repair

Reference

[1] He C, Zhang J, Bai X, Lu C, Zhang K. Lysine lactylation-based insight to understanding the characterization of cervical cancer.. Biochim Biophys Acta Mol Basis Dis 1870(7):167356. 2024 Oct. PMID: 39025375.

[2] Wu Q, Li Z, Gong T, Zheng X, Zhou X et al.. Porphyromonas gingivalis infection induces lysine lactylation reprogramming in human umbilical vein endothelial cells.. Front Cell Infect Microbiol 16:1706727. 2026. PMID: 41696360.