Search Results

Overview

Uniprot IDQ05193
Protein NameDynamin-1
Gene NameDNM1
OrganismHomo sapiens

Kla Sites from experimental identification

Position Flanking peptide
246 QKDIDGKKDITAALA
299 TLPGLRNKLQSQLLS
315 EKEVEEYKNFRPDDP
393 REISYAIKNIHGIRT
510 SNQMNKKKTSGNQDE
571 MLSVDNLKLRDVEKG
598 TEQRNVYKDYRQLEL
90 FLHCKGKKFTDFEEV

Function

Catalyzes the hydrolysis of GTP and utilizes this energy to mediate vesicle scission and participates in many forms of endocytosis, such as clathrin-mediated endocytosis or synaptic vesicle endocytosis as well as rapid endocytosis (RE) (PubMed:15703209, PubMed:20428113, PubMed:29668686, PubMed:8101525, PubMed:8910402, PubMed:9362482). Associates to the membrane, through lipid binding, and self-assembles into rings and stacks of interconnected rings through oligomerization to form a helical polymer around the vesicle membrane leading to constriction of invaginated coated pits around their necks (PubMed:30069048, PubMed:7877694, PubMed:9922133). Self-assembly of the helical polymer induces membrane tubules narrowing until the polymer reaches a length sufficient to trigger GTP hydrolysis (PubMed:19084269). Depending on the curvature imposed on the tubules, membrane detachment from the helical polymer upon GTP hydrolysis can cause spontaneous hemifission followed by complete fission (PubMed:19084269). May play a role in regulating early stages of clathrin-mediated endocytosis in non-neuronal cells through its activation by dephosphorylation via the signaling downstream of EGFR (PubMed:29668686). Controls vesicle size at a step before fission, during formation of membrane pits, at hippocampal synapses (By similarity). Controls plastic adaptation of the synaptic vesicle recycling machinery to high levels of activity (By similarity). Mediates rapid endocytosis (RE), a Ca(2+)-dependent and clathrin- and K(+)-independent process in chromaffin cells (By similarity). Microtubule-associated force-producing protein involved in producing microtubule bundles and able to bind and hydrolyze GTP (By similarity). Through its interaction with DNAJC6, acts during the early steps of clathrin-coated vesicle (CCV) formation (PubMed:12791276)

Protein Sequence

10 MGNRGMEDLI 20 PLVNRLQDAF 30 SAIGQNADLD 40 LPQIAVVGGQ 50 SAGKSSVLEN 60 FVGRDFLPRG 70 SGIVTRRPLV 80 LQLVNATTEY 90 AEFLHCKGKK 100 FTDFEEVRLE 110 IEAETDRVTG 120 TNKGISPVPI 130 NLRVYSPHVL 140 NLTLVDLPGM 150 TKVPVGDQPP 160 DIEFQIRDML 170 MQFVTKENCL 180 ILAVSPANSD 190 LANSDALKVA 200 KEVDPQGQRT 210 IGVITKLDLM 220 DEGTDARDVL 230 ENKLLPLRRG 240 YIGVVNRSQK 250 DIDGKKDITA 260 ALAAERKFFL 270 SHPSYRHLAD 280 RMGTPYLQKV 290 LNQQLTNHIR 300 DTLPGLRNKL 310 QSQLLSIEKE 320 VEEYKNFRPD 330 DPARKTKALL 340 QMVQQFAVDF 350 EKRIEGSGDQ 360 IDTYELSGGA 370 RINRIFHERF 380 PFELVKMEFD 390 EKELRREISY 400 AIKNIHGIRT 410 GLFTPDMAFE 420 TIVKKQVKKI 430 REPCLKCVDM 440 VISELISTVR 450 QCTKKLQQYP 460 RLREEMERIV 470 TTHIREREGR 480 TKEQVMLLID 490 IELAYMNTNH 500 EDFIGFANAQ 510 QRSNQMNKKK 520 TSGNQDEILV 530 IRKGWLTINN 540 IGIMKGGSKE 550 YWFVLTAENL 560 SWYKDDEEKE 570 KKYMLSVDNL 580 KLRDVEKGFM 590 SSKHIFALFN 600 TEQRNVYKDY 610 RQLELACETQ 620 EEVDSWKASF 630 LRAGVYPERV 640 GDKEKASETE 650 ENGSDSFMHS 660 MDPQLERQVE 670 TIRNLVDSYM 680 AIVNKTVRDL 690 MPKTIMHLMI 700 NNTKEFIFSE 710 LLANLYSCGD 720 QNTLMEESAE 730 QAQRRDEMLR 740 MYHALKEALS 750 IIGDINTTTV 760 STPMPPPVDD 770 SWLQVQSVPA 780 GRRSPTSSPT 790 PQRRAPAVPP 800 ARPGSRGPAP 810 GPPPAGSALG 820 GAPPVPSRPG 830 ASPDPFGPPP 840 QVPSRPNRAP 850 PGVPSRSGQA 860 SPSRPESPRP PFDL

Gene Ontology

Classification GO ID Description
Cellular Component GO:0042583 chromaffin granule
Cellular Component GO:0005905 clathrin-coated pit
Cellular Component GO:0005737 cytoplasm
Cellular Component GO:0030139 endocytic vesicle
Cellular Component GO:0070062 extracellular exosome
Cellular Component GO:0005874 microtubule
Cellular Component GO:0005886 plasma membrane
Cellular Component GO:0098793 presynapse
Cellular Component GO:0045202 synapse
Molecular Function GO:0019003 GDP binding
Molecular Function GO:0005525 GTP binding
Molecular Function GO:0003924 GTPase activity
Molecular Function GO:0042802 identical protein binding
Molecular Function GO:0008017 microtubule binding
Molecular Function GO:0005547 phosphatidylinositol-3,4,5-trisphosphate binding
Molecular Function GO:0005546 phosphatidylinositol-4,5-bisphosphate binding
Molecular Function GO:0042803 protein homodimerization activity
Molecular Function GO:0019901 protein kinase binding
Molecular Function GO:0003723 RNA binding
Biological Process GO:0099049 clathrin coat assembly involved in endocytosis
Biological Process GO:0006897 endocytosis
Biological Process GO:0007032 endosome organization
Biological Process GO:0051260 protein homooligomerization
Biological Process GO:0051289 protein homotetramerization
Biological Process GO:0006898 receptor-mediated endocytosis
Biological Process GO:0097494 regulation of vesicle size
Biological Process GO:0016185 synaptic vesicle budding from presynaptic endocytic zone membrane
Biological Process GO:0099050 vesicle scission

Reference

[1] Cheng Z, Huang H, Li M, Chen Y. Proteomic analysis identifies PFKP lactylation in SW480 colon cancer cells.. iScience 27(1):108645. 2024 Jan 19. PMID: 38155775.

[2] Shi CM, Wang QC, Li XL, Yang YH, Tang XY et al.. Global Profiling of Protein Lactylation in Human Hippocampi.. Proteomics Clin Appl 19(2):e202400061. 2025 Mar. PMID: 39610256.