Overview
| Uniprot ID | Q05BQ5 |
|---|
| Protein Name | MBT domain-containing protein 1 |
|---|
| Gene Name | MBTD1 |
|---|
| Organism | Homo sapiens |
|---|
Kla Sites from experimental identification
| Position |
Flanking peptide |
| 109 |
KKAKVLQKQPLVAKL |
Function
Chromatin reader component of the NuA4 histone acetyltransferase complex, a multiprotein complex involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A (PubMed:27153538, PubMed:32209463). The NuA4 complex plays a direct role in repair of DNA double-strand breaks (DSBs) by promoting homologous recombination (HR) (PubMed:27153538). MBTD1 specifically recognizes and binds monomethylated and dimethylated 'Lys-20' on histone H4 (H4K20me1 and H4K20me2, respectively) (PubMed:19841675, PubMed:27153538, PubMed:32209463). In the NuA4 complex, MBTD1 promotes recruitment of the complex to H4K20me marks by competing with TP53BP1 for binding to H4K20me (PubMed:27153538). Following recruitment to H4K20me at DNA breaks, the NuA4 complex catalyzes acetylation of 'Lys-15' on histone H2A (H2AK15), blocking the ubiquitination mark required for TP53BP1 localization at DNA breaks, thereby promoting homologous recombination (HR) (PubMed:27153538)
Protein Sequence
10
MFDGYDSCSE
20
DTSSSSSSEE
30
SEEEVAPLPS
40
NLPIIKNNGQ
50
VYTYPDGKSG
60
MATCEMCGMV
70
GVRDAFYSKT
80
KRFCSVSCSR
90
SYSSNSKKAS
100
ILARLQGKPP
110
TKKAKVLQKQ
120
PLVAKLAAYA
130
QYQATLQNQA
140
KTKAAVSMEG
150
FSWGNYINSN
160
SFIAAPVTCF
170
KHAPMGTCWG
180
DISENVRVEV
190
PNTDCSLPTK
200
VFWIAGIVKL
210
AGYNALLRYE
220
GFENDSGLDF
230
WCNICGSDIH
240
PVGWCAASGK
250
PLVPPRTIQH
260
KYTNWKAFLV
270
KRLTGAKTLP
280
PDFSQKVSES
290
MQYPFKPCMR
300
VEVVDKRHLC
310
RTRVAVVESV
320
IGGRLRLVYE
330
ESEDRTDDFW
340
CHMHSPLIHH
350
IGWSRSIGHR
360
FKRSDITKKQ
370
DGHFDTPPHL
380
FAKVKEVDQS
390
GEWFKEGMKL
400
EAIDPLNLST
410
ICVATIRKVL
420
ADGFLMIGID
430
GSEAADGSDW
440
FCYHATSPSI
450
FPVGFCEINM
460
IELTPPRGYT
470
KLPFKWFDYL
480
RETGSIAAPV
490
KLFNKDVPNH
500
GFRVGMKLEA
510
VDLMEPRLIC
520
VATVTRIIHR
530
LLRIHFDGWE
540
EEYDQWVDCE
550
SPDLYPVGWC
560
QLTGYQLQPP
570
ASQSSRENQS
580
ASSKQKKKAK
590
SQQYKGHKKM
600
TTLQLKEELL
610
DGEDYNFLQG
620
ASDQESNGSA
NFYIKQEP
Gene Ontology
| Classification |
GO ID |
Description |
| Cellular Component |
GO:0035267 |
NuA4 histone acetyltransferase complex |
| Biological Process |
GO:0045892 |
negative regulation of DNA-templated transcription |
| Biological Process |
GO:0045893 |
positive regulation of DNA-templated transcription |
| Biological Process |
GO:1905168 |
positive regulation of double-strand break repair via homologous recombination |
| Biological Process |
GO:0042981 |
regulation of apoptotic process |
| Biological Process |
GO:0051726 |
regulation of cell cycle |
| Biological Process |
GO:2000779 |
regulation of double-strand break repair |
| Cellular Component |
GO:0005654 |
nucleoplasm |
| Cellular Component |
GO:0005634 |
nucleus |
| Cellular Component |
GO:0035861 |
site of double-strand break |
| Molecular Function |
GO:0003682 |
chromatin binding |
| Molecular Function |
GO:0042393 |
histone binding |
| Molecular Function |
GO:0140117 |
histone H4K20me1 reader activity |
| Molecular Function |
GO:0140005 |
histone H4K20me2 reader activity |
| Molecular Function |
GO:0062060 |
NuA4 histone acetyltransferase complex binding |
| Molecular Function |
GO:0008270 |
zinc ion binding |
| Biological Process |
GO:0006351 |
DNA-templated transcription |
| Biological Process |
GO:0000724 |
double-strand break repair via homologous recombination |
Reference
PMID: N/A