Search Results

Overview

Uniprot ID	Q07157
Protein Name	Tight junction protein 1
Gene Name	TJP1
Organism	Homo sapiens

Kla Sites from experimental identification

Position	Flanking peptide
1005	DPTKVYRKDPYPEEM
153	VVNRRSEKIWPRDRS
194	VTLVKSRKNEEYGLR
257	ERSKGKLKMVVQRDE
9	SARAAAAKSTAMEET

Function

TJP1, TJP2, and TJP3 are closely related scaffolding proteins that link tight junction (TJ) transmembrane proteins such as claudins, junctional adhesion molecules, and occludin to the actin cytoskeleton (PubMed:7798316, PubMed:9792688). Forms a multistranded TJP1/ZO1 condensate which elongates to form a tight junction belt, the belt is anchored at the apical cell membrane via interaction with PATJ (By similarity). The tight junction acts to limit movement of substances through the paracellular space and as a boundary between the compositionally distinct apical and basolateral plasma membrane domains of epithelial and endothelial cells. Necessary for lumenogenesis, and particularly efficient epithelial polarization and barrier formation (By similarity). Plays a role in the regulation of cell migration by targeting CDC42BPB to the leading edge of migrating cells (PubMed:21240187). Plays an important role in podosome formation and associated function, thus regulating cell adhesion and matrix remodeling (PubMed:20930113). With TJP2 and TJP3, participates in the junctional retention and stability of the transcription factor DBPA, but is not involved in its shuttling to the nucleus (By similarity). May play a role in mediating cell morphology changes during ameloblast differentiation via its role in tight junctions (By similarity)

Protein Sequence

10 MSARAAAAKS 20 TAMEETAIWE 30 QHTVTLHRAP 40 GFGFGIAISG 50 GRDNPHFQSG 60 ETSIVISDVL 70 KGGPAEGQLQ 80 ENDRVAMVNG 90 VSMDNVEHAF 100 AVQQLRKSGK 110 NAKITIRRKK 120 KVQIPVSRPD 130 PEPVSDNEED 140 SYDEEIHDPR 150 SGRSGVVNRR 160 SEKIWPRDRS 170 ASRERSLSPR 180 SDRRSVASSQ 190 PAKPTKVTLV 200 KSRKNEEYGL 210 RLASHIFVKE 220 ISQDSLAARD 230 GNIQEGDVVL 240 KINGTVTENM 250 SLTDAKTLIE 260 RSKGKLKMVV 270 QRDERATLLN 280 VPDLSDSIHS 290 ANASERDDIS 300 EIQSLASDHS 310 GRSHDRPPRR 320 SRSRSPDQRS 330 EPSDHSRHSP 340 QQPSNGSLRS 350 RDEERISKPG 360 AVSTPVKHAD 370 DHTPKTVEEV 380 TVERNEKQTP 390 SLPEPKPVYA 400 QVGQPDVDLP 410 VSPSDGVLPN 420 STHEDGILRP 430 SMKLVKFRKG 440 DSVGLRLAGG 450 NDVGIFVAGV 460 LEDSPAAKEG 470 LEEGDQILRV 480 NNVDFTNIIR 490 EEAVLFLLDL 500 PKGEEVTILA 510 QKKKDVYRRI 520 VESDVGDSFY 530 IRTHFEYEKE 540 SPYGLSFNKG 550 EVFRVVDTLY 560 NGKLGSWLAI 570 RIGKNHKEVE 580 RGIIPNKNRA 590 EQLASVQYTL 600 PKTAGGDRAD 610 FWRFRGLRSS 620 KRNLRKSRED 630 LSAQPVQTKF 640 PAYERVVLRE 650 AGFLRPVTIF 660 GPIADVAREK 670 LAREEPDIYQ 680 IAKSEPRDAG 690 TDQRSSGIIR 700 LHTIKQIIDQ 710 DKHALLDVTP 720 NAVDRLNYAQ 730 WYPIVVFLNP 740 DSKQGVKTMR 750 MRLCPESRKS 760 ARKLYERSHK 770 LRKNNHHLFT 780 TTINLNSMND 790 GWYGALKEAI 800 QQQQNQLVWV 810 SEGKADGATS 820 DDLDLHDDRL 830 SYLSAPGSEY 840 SMYSTDSRHT 850 SDYEDTDTEG 860 GAYTDQELDE 870 TLNDEVGTPP 880 ESAITRSSEP 890 VREDSSGMHH 900 ENQTYPPYSP 910 QAQPQPIHRI 920 DSPGFKPASQ 930 QKAEASSPVP 940 YLSPETNPAS 950 STSAVNHNVN 960 LTNVRLEEPT 970 PAPSTSYSPQ 980 ADSLRTPSTE 990 AAHIMLRDQE 1000 PSLSSHVDPT 1010 KVYRKDPYPE 1020 EMMRQNHVLK 1030 QPAVSHPGHR 1040 PDKEPNLTYE 1050 PQLPYVEKQA 1060 SRDLEQPTYR 1070 YESSSYTDQF 1080 SRNYEHRLRY 1090 EDRVPMYEEQ 1100 WSYYDDKQPY 1110 PSRPPFDNQH 1120 SQDLDSRQHP 1130 EESSERGYFP 1140 RFEEPAPLSY 1150 DSRPRYEQAP 1160 RASALRHEEQ 1170 PAPGYDTHGR 1180 LRPEAQPHPS 1190 AGPKPAESKQ 1200 YFEQYSRSYE 1210 QVPPQGFTSR 1220 AGHFEPLHGA 1230 AAVPPLIPSS 1240 QHKPEALPSN 1250 TKPLPPPPTQ 1260 TEEEEDPAMK 1270 PQSVLTRVKM 1280 FENKRSASLE 1290 TKKDVNDTGS 1300 FKPPEVASKP 1310 SGAPIIGPKP 1320 TSQNQFSEHD 1330 KTLYRIPEPQ 1340 KPQLKPPEDI 1350 VRSNHYDPEE 1360 DEEYYRKQLS 1370 YFDRRSFENK 1380 PPAHIAASHL 1390 SEPAKPAHSQ 1400 NQSNFSSYSS 1410 KGKPPEADGV 1420 DRSFGEKRYE 1430 PIQATPPPPP 1440 LPSQYAQPSQ 1450 PVTSASLHIH 1460 SKGAHGEGNS 1470 VSLDFQNSLV 1480 SKPDPPPSQN 1490 KPATFRPPNR 1500 EDTAQAAFYP 1510 QKSFPDKAPV 1520 NGTEQTQKTV 1530 TPAYNRFTPK 1540 PYTSSARPFE 1550 RKFESPKFNH 1560 NLLPSETAHK 1570 PDLSSKTPTS 1580 PKTLVKSHSL 1590 AQPPEFDSGV 1600 ETFSIHAEKP 1610 KYQINNISTV 1620 PKAIPVSPSA 1630 VEEDEDEDGH 1640 TVVATARGIF 1650 NSNGGVLSSI 1660 ETGVSIIIPQ 1670 GAIPEGVEQE 1680 IYFKVCRDNS 1690 ILPPLDKEKG 1700 ETLLSPLVMC 1710 GPHGLKFLKP 1720 VELRLPHCDP 1730 KTWQNKCLPG 1740 DPNYLVGANC VSVLIDHF

Gene Ontology

Classification	GO ID	Description
Cellular Component	GO:0005912	adherens junction
Cellular Component	GO:0043296	apical junction complex
Cellular Component	GO:0045177	apical part of cell
Cellular Component	GO:0016323	basolateral plasma membrane
Cellular Component	GO:0005923	bicellular tight junction
Cellular Component	GO:0030054	cell junction
Cellular Component	GO:0005737	cytoplasm
Cellular Component	GO:0005829	cytosol
Cellular Component	GO:0005921	gap junction
Cellular Component	GO:0005886	plasma membrane
Cellular Component	GO:0002102	podosome
Cellular Component	GO:0032991	protein-containing complex
Cellular Component	GO:0070160	tight junction
Molecular Function	GO:0045296	cadherin binding
Molecular Function	GO:0005516	calmodulin binding
Molecular Function	GO:0050839	cell adhesion molecule binding
Biological Process	GO:0030036	actin cytoskeleton organization
Biological Process	GO:0031032	actomyosin structure organization
Biological Process	GO:0034334	adherens junction maintenance
Biological Process	GO:0036305	ameloblast differentiation
Biological Process	GO:0098609	cell-cell adhesion
Biological Process	GO:0007043	cell-cell junction assembly
Biological Process	GO:0045216	cell-cell junction organization
Biological Process	GO:0090557	establishment of endothelial intestinal barrier
Biological Process	GO:0035633	maintenance of blood-brain barrier
Biological Process	GO:0043066	negative regulation of apoptotic process
Biological Process	GO:0051497	negative regulation of stress fiber assembly
Biological Process	GO:1905605	positive regulation of blood-brain barrier permeability
Biological Process	GO:0030335	positive regulation of cell migration
Biological Process	GO:0008284	positive regulation of cell population proliferation
Biological Process	GO:2000049	positive regulation of cell-cell adhesion mediated by cadherin
Biological Process	GO:1903672	positive regulation of sprouting angiogenesis
Biological Process	GO:0071896	protein localization to adherens junction
Biological Process	GO:1902396	protein localization to bicellular tight junction
Biological Process	GO:0150105	protein localization to cell-cell junction
Biological Process	GO:2000810	regulation of bicellular tight junction assembly
Biological Process	GO:1901888	regulation of cell junction assembly
Biological Process	GO:0051493	regulation of cytoskeleton organization

Reference

[1] Yang Z, Yan C, Ma J, Peng P, Ren X et al.. Lactylome analysis suggests lactylation-dependent mechanisms of metabolic adaptation in hepatocellular carcinoma.. Nat Metab 5(1):61-79. 2023 Jan. PMID: 36593272.

[2] Shi CM, Wang QC, Li XL, Yang YH, Tang XY et al.. Global Profiling of Protein Lactylation in Human Hippocampi.. Proteomics Clin Appl 19(2):e202400061. 2025 Mar. PMID: 39610256.

[3] He J, Lai T, Zhou Z, Yang H, Lei Z et al.. Multiomics profiling reveals the involvement of protein lactylation in nonhomologous end joining pathway conferring radioresistance in lung adenocarcinoma cell.. Sci Rep 15(1):24651. 2025 Jul 9. PMID: 40634431.

[4] Wu Q, Li Z, Gong T, Zheng X, Zhou X et al.. Porphyromonas gingivalis infection induces lysine lactylation reprogramming in human umbilical vein endothelial cells.. Front Cell Infect Microbiol 16:1706727. 2026. PMID: 41696360.