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Overview

Uniprot ID	Q07205
Protein Name	Eukaryotic translation initiation factor 5
Gene Name	Eif5
Organism	Rattus norvegicus

Kla Sites from experimental identification

Position	Flanking peptide
114	TDLHVNPKKQTIGNS
411	SKTASVPKVETVKSD

Function

Component of the 43S pre-initiation complex (43S PIC), which binds to the mRNA cap-proximal region, scans mRNA 5'-untranslated region, and locates the initiation codon. In this complex, acts as a GTPase-activating protein, by promoting GTP hydrolysis by eIF2G (EIF2S3). During scanning, interacts with both EIF1 (via its C-terminal domain (CTD)) and EIF1A (via its NTD). This interaction with EIF1A contributes to the maintenance of EIF1 within the open 43S PIC. When start codon is recognized, EIF5, via its NTD, induces eIF2G (EIF2S3) to hydrolyze the GTP. Start codon recognition also induces a conformational change of the PIC to a closed state. This change increases the affinity of EIF5-CTD for EIF2-beta (EIF2S2), which allows the release, by an indirect mechanism, of EIF1 from the PIC. Finally, EIF5 stabilizes the PIC in its closed conformation

Protein Sequence

10 MSVNVNRSVS 20 DQFYRYKMPR 30 LIAKVEGKGN 40 GIKTVIVNMV 50 DVAKALNRPP 60 TYPTKYFGCE 70 LGAQTQFDVK 80 NDRYIVNGSH 90 EANKLQDMLD 100 GFIKKFVLCP 110 ECENPETDLH 120 VNPKKQTIGN 130 SCKACGYRGM 140 LDTHHKLCTF 150 ILKNPPENSD 160 IGTGKKEKEK 170 KNRKGKDKEN 180 GSVSTSETPP 190 PPPPNEISPP 200 HAVEEEEDDD 210 WGEDTTEEAQ 220 RRRMDEISDH 230 AKGLTLSDDL 240 ERTVEERVNI 250 LFDFVKKKKE 260 EGIIDSSDKD 270 IVAEAERLDV 280 KAMGPLVLTE 290 VLFDEKIREQ 300 IKKYRRHFLR 310 FCHNNKKAQR 320 YLLHGLECVV 330 AMHQAQLISK 340 IPHILKEMYD 350 ADLLEEEVII 360 SWSEKASKKY 370 VSKELAKEIR 380 VKAEPFIKWL 390 KEAEEESSGG 400 EEEDEDENIE 410 VVYSKTASVP 420 KVETVKSDNK DDDIDIDAI

Gene Ontology

Classification	GO ID	Description
Cellular Component	GO:0005737	cytoplasm
Cellular Component	GO:0005829	cytosol
Cellular Component	GO:0045202	synapse
Molecular Function	GO:0071074	eukaryotic initiation factor eIF2 binding
Molecular Function	GO:0005092	GDP-dissociation inhibitor activity
Molecular Function	GO:0005525	GTP binding
Molecular Function	GO:0005096	GTPase activator activity
Molecular Function	GO:0003743	translation initiation factor activity
Biological Process	GO:0090630	activation of GTPase activity
Biological Process	GO:0001732	formation of cytoplasmic translation initiation complex
Biological Process	GO:0001731	formation of translation preinitiation complex
Biological Process	GO:0006446	regulation of translational initiation
Biological Process	GO:0042255	ribosome assembly

Reference

[1] Sheng L, Xu H, Wang Y, Ni J, Xiang T et al.. Systematic analysis of lysine lactylation in nucleus pulposus cells.. iScience 27(11):111157. 2024 Nov 15. PMID: 39524337.

[2] Chen Y, Sun W, Sun Z, Zhao H, Wu T et al.. Effect of electroacupuncture on hippocampal protein lactylation in a rat model of vascular dementia.. Front Neurol 16:1629474. 2025. PMID: 40963935.