Search Results

Overview

Uniprot IDQ08257
Protein NameZeta-crystallin
Gene NameCRYZ
OrganismHomo sapiens

Kla Sites from experimental identification

Position Flanking peptide
120 TVYKLPEKLDFKQGA
175 IARAYGLKILGTAGT
187 AGTEEGQKIVLQNGA
208 REVNYIDKIKKYVGE
23 FGGPEVLKLRSDIAV
307 GSQYPLEKVAEAHEN
34 DIAVPIPKDHQVLIK
6 **MATGQKLMRAVRV
62 RSGTYSRKPLLPYTP

Function

Multifunctional crystallin endowed with unrelated mRNA stabilization and enzymatic oxidoreductase activities (PubMed:17497241, PubMed:20103721). Binds (AU)-rich elements (ARE) in the 3'-UTR of target mRNA to enhance their stability (PubMed:17497241, PubMed:20103721). Upon metabolic acidosis, stabilizes the mRNA encoding SLC12A1 a cotransporter involved in transepithelial NH4(+) reabsorption in the medullary thick ascending limb in kidney (By similarity). In response to acidosis, also participates to the adaptive increase in renal ammoniagenesis by stabilizing the mRNAs of enzymes involved in glutamine catabolism (By similarity). By the same mechanism, it also regulates the expression of the antiapoptotic protein BCL2 (PubMed:20103721). Beside its mRNA stabilization activity, also catalyzes the reduction of orthoquinones such as 1,2-naphthoquinone or 9,10-phenanthrenequinone in the presence of NADPH in vitro and could be involved in the detoxification of xenobiotics and protection against oxidative stress (PubMed:17497241)

Protein Sequence

10 MATGQKLMRA 20 VRVFEFGGPE 30 VLKLRSDIAV 40 PIPKDHQVLI 50 KVHACGVNPV 60 ETYIRSGTYS 70 RKPLLPYTPG 80 SDVAGVIEAV 90 GDNASAFKKG 100 DRVFTSSTIS 110 GGYAEYALAA 120 DHTVYKLPEK 130 LDFKQGAAIG 140 IPYFTAYRAL 150 IHSACVKAGE 160 SVLVHGASGG 170 VGLAACQIAR 180 AYGLKILGTA 190 GTEEGQKIVL 200 QNGAHEVFNH 210 REVNYIDKIK 220 KYVGEKGIDI 230 IIEMLANVNL 240 SKDLSLLSHG 250 GRVIVVGSRG 260 TIEINPRDTM 270 AKESSIIGVT 280 LFSSTKEEFQ 290 QYAAALQAGM 300 EIGWLKPVIG 310 SQYPLEKVAE 320 AHENIIHGSG ATGKMILLL

Gene Ontology

Classification GO ID Description
Cellular Component GO:0005829 cytosol
Cellular Component GO:0070062 extracellular exosome
Cellular Component GO:0005739 mitochondrion
Molecular Function GO:0042802 identical protein binding
Molecular Function GO:0003730 mRNA 3'-UTR binding
Molecular Function GO:0070402 NADPH binding
Molecular Function GO:0003960 quinone reductase (NADPH) activity
Molecular Function GO:0008270 zinc ion binding
Biological Process GO:0048255 mRNA stabilization
Biological Process GO:0051289 protein homotetramerization
Biological Process GO:0007601 visual perception
Biological Process GO:0042178 xenobiotic catabolic process

Reference

[1] Yang Z, Yan C, Ma J, Peng P, Ren X et al.. Lactylome analysis suggests lactylation-dependent mechanisms of metabolic adaptation in hepatocellular carcinoma.. Nat Metab 5(1):61-79. 2023 Jan. PMID: 36593272.

[2] Hong H, Chen X, Wang H, Gu X, Yuan Y et al.. Global profiling of protein lysine lactylation and potential target modified protein analysis in hepatocellular carcinoma.. Proteomics 23(9):e2200432. 2023 May. PMID: 36625413.

[3] Shi CM, Wang QC, Li XL, Yang YH, Tang XY et al.. Global Profiling of Protein Lactylation in Human Hippocampi.. Proteomics Clin Appl 19(2):e202400061. 2025 Mar. PMID: 39610256.