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Overview

Uniprot IDQ08426
Protein NamePeroxisomal bifunctional enzyme
Gene NameEHHADH
OrganismHomo sapiens

Kla Sites from experimental identification

Position Flanking peptide
165 ILADEALKLGILDKV
250 YPYEVGIKKEEELFL
280 FAERKANKWSTPSGA
290 TPSGASWKTASARPV
33 TTLLRDIKEGLQKAV
330 VIAVDSDKNQLATAN
338 NQLATANKMITSVLE
346 MITSVLEKEASKMQQ
350 VLEKEASKMQQSGHP
362 GHPWSGPKPRLTSSV
38 DIKEGLQKAVIDHTI
46 AVIDHTIKAIVICGA
532 AGLDVGWKSRKGQGL
535 DVGWKSRKGQGLTGP
574 ELGRFGQKTGKGWYQ
577 RFGQKTGKGWYQYDK
584 KGWYQYDKPLGRIHK
591 KPLGRIHKPDPWLSK
598 KPDPWLSKFLSRYRK
605 KFLSRYRKTHHIEPR
660 GYGWPRHKGGPMFYA
700 EPSDYLKKLASQGNP
710 SQGNPPLKEWQSLAG
722 LAGSPSSKL******

Function

Peroxisomal trifunctional enzyme possessing 2-enoyl-CoA hydratase, 3-hydroxyacyl-CoA dehydrogenase, and delta 3, delta 2-enoyl-CoA isomerase activities. Catalyzes two of the four reactions of the long chain fatty acids peroxisomal beta-oxidation pathway (By similarity). Can also use branched-chain fatty acids such as 2-methyl-2E-butenoyl-CoA as a substrate, which is hydrated into (2S,3S)-3-hydroxy-2-methylbutanoyl-CoA (By similarity). Optimal isomerase for 2,5 double bonds into 3,5 form isomerization in a range of enoyl-CoA species (Probable). Also able to isomerize both 3-cis and 3-trans double bonds into the 2-trans form in a range of enoyl-CoA species (By similarity). With HSD17B4, catalyzes the hydration of trans-2-enoyl-CoA and the dehydrogenation of 3-hydroxyacyl-CoA, but with opposite chiral specificity (PubMed:15060085). Regulates the amount of medium-chain dicarboxylic fatty acids which are essential regulators of all fatty acid oxidation pathways (By similarity). Also involved in the degradation of long-chain dicarboxylic acids through peroxisomal beta-oxidation (PubMed:15060085)

Protein Sequence

10 MAEYTRLHNA 20 LALIRLRNPP 30 VNAISTTLLR 40 DIKEGLQKAV 50 IDHTIKAIVI 60 CGAEGKFSAG 70 ADIRGFSAPR 80 TFGLTLGHVV 90 DEIQRNEKPV 100 VAAIQGMAFG 110 GGLELALGCH 120 YRIAHAEAQV 130 GLPEVTLGLL 140 PGARGTQLLP 150 RLTGVPAALD 160 LITSGRRILA 170 DEALKLGILD 180 KVVNSDPVEE 190 AIRFAQRVSD 200 QPLESRRLCN 210 KPIQSLPNMD 220 SIFSEALLKM 230 RRQHPGCLAQ 240 EACVRAVQAA 250 VQYPYEVGIK 260 KEEELFLYLL 270 QSGQARALQY 280 AFFAERKANK 290 WSTPSGASWK 300 TASARPVSSV 310 GVVGLGTMGR 320 GIVISFARAR 330 IPVIAVDSDK 340 NQLATANKMI 350 TSVLEKEASK 360 MQQSGHPWSG 370 PKPRLTSSVK 380 ELGGVDLVIE 390 AVFEEMSLKK 400 QVFAELSAVC 410 KPEAFLCTNT 420 SALDVDEIAS 430 STDRPHLVIG 440 THFFSPAHVM 450 KLLEVIPSQY 460 SSPTTIATVM 470 NLSKKIKKIG 480 VVVGNCFGFV 490 GNRMLNPYYN 500 QAYFLLEEGS 510 KPEEVDQVLE 520 EFGFKMGPFR 530 VSDLAGLDVG 540 WKSRKGQGLT 550 GPTLLPGTPA 560 RKRGNRRYCP 570 IPDVLCELGR 580 FGQKTGKGWY 590 QYDKPLGRIH 600 KPDPWLSKFL 610 SRYRKTHHIE 620 PRTISQDEIL 630 ERCLYSLINE 640 AFRILGEGIA 650 ASPEHIDVVY 660 LHGYGWPRHK 670 GGPMFYASTV 680 GLPTVLEKLQ 690 KYYRQNPDIP 700 QLEPSDYLKK 710 LASQGNPPLK 720 EWQSLAGSPS SKL

Gene Ontology

Classification GO ID Description
Cellular Component GO:0005829 cytosol
Cellular Component GO:0005782 peroxisomal matrix
Cellular Component GO:0005777 peroxisome
Molecular Function GO:0003857 (3S)-3-hydroxyacyl-CoA dehydrogenase (NAD+) activity
Molecular Function GO:0018812 3-hydroxyacyl-CoA dehydratase activity
Molecular Function GO:0004165 delta(3)-delta(2)-enoyl-CoA isomerase activity
Molecular Function GO:0004300 enoyl-CoA hydratase activity
Molecular Function GO:0019899 enzyme binding
Molecular Function GO:0016863 intramolecular oxidoreductase activity, transposing C=C bonds
Molecular Function GO:0016509 long-chain (3S)-3-hydroxyacyl-CoA dehydrogenase (NAD+) activity
Molecular Function GO:0070403 NAD+ binding
Biological Process GO:0006635 fatty acid beta-oxidation
Biological Process GO:0033540 fatty acid beta-oxidation using acyl-CoA oxidase
Biological Process GO:0006633 fatty acid biosynthetic process

Reference

[1] Yang Z, Yan C, Ma J, Peng P, Ren X et al.. Lactylome analysis suggests lactylation-dependent mechanisms of metabolic adaptation in hepatocellular carcinoma.. Nat Metab 5(1):61-79. 2023 Jan. PMID: 36593272.

[2] Hong H, Chen X, Wang H, Gu X, Yuan Y et al.. Global profiling of protein lysine lactylation and potential target modified protein analysis in hepatocellular carcinoma.. Proteomics 23(9):e2200432. 2023 May. PMID: 36625413.

[3] Lin Y, Chen M, Wang D, Yu Y, Chen R et al.. Multi-Proteomic Analysis Reveals the Effect of Protein Lactylation on Matrix and Cholesterol Metabolism in Tendinopathy.. J Proteome Res 22(6):1712-1722. 2023 Jun 2. PMID: 37159428.