Search Results

Overview

Uniprot IDQ12874
Protein NameSplicing factor 3A subunit 3
Gene NameSF3A3
OrganismHomo sapiens

Kla Sites from experimental identification

Position Flanking peptide
303 LDTSLFAKNPKSKGT
306 SLFAKNPKSKGTKRD
308 FAKNPKSKGTKRDTE
489 SSGNVVNKKTYEDLK
490 SGNVVNKKTYEDLKR
496 KKTYEDLKRQGLL**

Function

Component of the 17S U2 SnRNP complex of the spliceosome, a large ribonucleoprotein complex that removes introns from transcribed pre-mRNAs (PubMed:10882114, PubMed:11533230, PubMed:32494006, PubMed:34822310, PubMed:8022796). The 17S U2 SnRNP complex (1) directly participates in early spliceosome assembly and (2) mediates recognition of the intron branch site during pre-mRNA splicing by promoting the selection of the pre-mRNA branch-site adenosine, the nucleophile for the first step of splicing (PubMed:10882114, PubMed:11533230, PubMed:32494006, PubMed:34822310). Within the 17S U2 SnRNP complex, SF3A3 is part of the SF3A subcomplex that contributes to the assembly of the 17S U2 snRNP, and the subsequent assembly of the pre-spliceosome 'E' complex and the pre-catalytic spliceosome 'A' complex (PubMed:10882114, PubMed:11533230). Involved in pre-mRNA splicing as a component of pre-catalytic spliceosome 'B' complexes (PubMed:29360106, PubMed:30315277)

Protein Sequence

10 METILEQQRR 20 YHEEKERLMD 30 VMAKEMLTKK 40 STLRDQINSD 50 HRTRAMQDRY 60 MEVSGNLRDL 70 YDDKDGLRKE 80 ELNAISGPNE 90 FAEFYNRLKQ 100 IKEFHRKHPN 110 EICVPMSVEF 120 EELLKARENP 130 SEEAQNLVEF 140 TDEEGYGRYL 150 DLHDCYLKYI 160 NLKASEKLDY 170 ITYLSIFDQL 180 FDIPKERKNA 190 EYKRYLEMLL 200 EYLQDYTDRV 210 KPLQDQNELF 220 GKIQAEFEKK 230 WENGTFPGWP 240 KETSSALTHA 250 GAHLDLSAFS 260 SWEELASLGL 270 DRLKSALLAL 280 GLKCGGTLEE 290 RAQRLFSTKG 300 KSLESLDTSL 310 FAKNPKSKGT 320 KRDTERNKDI 330 AFLEAQIYEY 340 VEILGEQRHL 350 THENVQRKQA 360 RTGEEREEEE 370 EEQISESESE 380 DEENEIIYNP 390 KNLPLGWDGK 400 PIPYWLYKLH 410 GLNINYNCEI 420 CGNYTYRGPK 430 AFQRHFAEWR 440 HAHGMRCLGI 450 PNTAHFANVT 460 QIEDAVSLWA 470 KLKLQKASER 480 WQPDTEEEYE 490 DSSGNVVNKK 500 TYEDLKRQGL L

Gene Ontology

Classification GO ID Description
Cellular Component GO:0071013 catalytic step 2 spliceosome
Cellular Component GO:0016607 nuclear speck
Cellular Component GO:0005654 nucleoplasm
Cellular Component GO:0005634 nucleus
Cellular Component GO:0005681 spliceosomal complex
Cellular Component GO:0005686 U2 snRNP
Cellular Component GO:0071005 U2-type precatalytic spliceosome
Cellular Component GO:0005684 U2-type spliceosomal complex
Molecular Function GO:0003723 RNA binding
Molecular Function GO:0008270 zinc ion binding
Biological Process GO:0000389 mRNA 3'-splice site recognition
Biological Process GO:0006397 mRNA processing
Biological Process GO:0000398 mRNA splicing, via spliceosome
Biological Process GO:0000375 RNA splicing, via transesterification reactions
Biological Process GO:0000387 spliceosomal snRNP assembly
Biological Process GO:1903241 U2-type prespliceosome assembly

Reference

[1] Yang D, Yin J, Shan L, Yi X, Zhang W et al.. Identification of lysine-lactylated substrates in gastric cancer cells.. iScience 25(7):104630. 2022 Jul 15. PMID: 35800753.

[2] Yang Z, Yan C, Ma J, Peng P, Ren X et al.. Lactylome analysis suggests lactylation-dependent mechanisms of metabolic adaptation in hepatocellular carcinoma.. Nat Metab 5(1):61-79. 2023 Jan. PMID: 36593272.

[3] He C, Zhang J, Bai X, Lu C, Zhang K. Lysine lactylation-based insight to understanding the characterization of cervical cancer.. Biochim Biophys Acta Mol Basis Dis 1870(7):167356. 2024 Oct. PMID: 39025375.

[4] Bao Q, Wan N, He Z, Cao J, Yuan W et al.. Subcellular Proteomic Mapping of Lysine Lactylation.. J Am Soc Mass Spectrom 35(12):3221-3232. 2024 Dec 4. PMID: 39569522.

[5] Guo X, Ren X, Yan C, Huang H. Quantitative Proteomics Reveals the Role of Lysine Lactylation in Lenalidomide-Resistance in Multiple Myeloma Cells.. ACS Chem Biol 20(7):1728-1738. 2025 Jul 18. PMID: 40590393.

[6] Wu Q, Li Z, Gong T, Zheng X, Zhou X et al.. Porphyromonas gingivalis infection induces lysine lactylation reprogramming in human umbilical vein endothelial cells.. Front Cell Infect Microbiol 16:1706727. 2026. PMID: 41696360.