Search Results

Overview

Uniprot IDQ13011
Protein NameDelta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial
Gene NameECH1
OrganismHomo sapiens

Kla Sites from experimental identification

Position Flanking peptide
231 ELAFTARKMMADEAL
276 PVAVQSTKVNLLYSR
318 VQATTENKELKTVTF
327 LKTVTFSKL******
65 LRVTSAQKHVLHVQL
77 VQLNRPNKRNAMNKV
83 NKRNAMNKVFWREMV

Function

Auxiliary enzyme in the beta-oxidation of mono- and polyunsaturated fatty acids (PubMed:26474213). Together with the 2,4-dienoyl-CoA reductase (DECR) and the Enoyl-CoA delta isomerase 1 (ECI1) they allow reentrance of the enoyl-CoA into the beta-oxidation cycle (PubMed:26474213). Able to isomerize 3-trans,5-cis-dienoyl-CoA (3E,5Z-dienoyl-CoA) to 2-trans,4-trans-dienoyl-CoA (2E,4E-dienoyl-CoA), a crucial step in the beta-oxidation of unsaturated fatty acids with double bonds at odd position (By similarity) (PubMed:26474213). Based on its established catalytic mechanism, it is predicted to act also on other fatty acids besides those tested experimentally (Probable)

Protein Sequence

10 MAAGIVASRR 20 LRDLLTRRLT 30 GSNYPGLSIS 40 LRLTGSSAQE 50 EASGVALGEA 60 PDHSYESLRV 70 TSAQKHVLHV 80 QLNRPNKRNA 90 MNKVFWREMV 100 ECFNKISRDA 110 DCRAVVISGA 120 GKMFTAGIDL 130 MDMASDILQP 140 KGDDVARISW 150 YLRDIITRYQ 160 ETFNVIERCP 170 KPVIAAVHGG 180 CIGGGVDLVT 190 ACDIRYCAQD 200 AFFQVKEVDV 210 GLAADVGTLQ 220 RLPKVIGNQS 230 LVNELAFTAR 240 KMMADEALGS 250 GLVSRVFPDK 260 EVMLDAALAL 270 AAEISSKSPV 280 AVQSTKVNLL 290 YSRDHSVAES 300 LNYVASWNMS 310 MLQTQDLVKS 320 VQATTENKEL KTVTFSKL

Gene Ontology

Classification GO ID Description
Cellular Component GO:0005829 cytosol
Cellular Component GO:0070062 extracellular exosome
Cellular Component GO:0016020 membrane
Cellular Component GO:0005759 mitochondrial matrix
Cellular Component GO:0005739 mitochondrion
Cellular Component GO:0005782 peroxisomal matrix
Cellular Component GO:0005777 peroxisome
Molecular Function GO:0051750 delta(3,5)-delta(2,4)-dienoyl-CoA isomerase activity
Biological Process GO:0006635 fatty acid beta-oxidation

Reference

[1] Yang Z, Yan C, Ma J, Peng P, Ren X et al.. Lactylome analysis suggests lactylation-dependent mechanisms of metabolic adaptation in hepatocellular carcinoma.. Nat Metab 5(1):61-79. 2023 Jan. PMID: 36593272.

[2] Hong H, Chen X, Wang H, Gu X, Yuan Y et al.. Global profiling of protein lysine lactylation and potential target modified protein analysis in hepatocellular carcinoma.. Proteomics 23(9):e2200432. 2023 May. PMID: 36625413.

[3] He J, Lai T, Zhou Z, Yang H, Lei Z et al.. Multiomics profiling reveals the involvement of protein lactylation in nonhomologous end joining pathway conferring radioresistance in lung adenocarcinoma cell.. Sci Rep 15(1):24651. 2025 Jul 9. PMID: 40634431.