Search Results

Overview

Uniprot IDQ13107
Protein NameUbiquitin carboxyl-terminal hydrolase 4
Gene NameUSP4
OrganismHomo sapiens

Kla Sites from experimental identification

Position Flanking peptide
232 PRQTLQSKSSTAPSR
773 LQPQKKKKTTVALRD

Function

Deubiquitinating enzyme that removes conjugated ubiquitin from target proteins (PubMed:16316627, PubMed:16339847, PubMed:16472766, PubMed:20595234, PubMed:22347420, PubMed:25404403, PubMed:28604766, PubMed:30514904). Deubiquitinates PDPK1 (PubMed:22347420). Deubiquitinates TRIM21 (PubMed:16316627). Deubiquitinates receptor ADORA2A which increases the amount of functional receptor at the cell surface (PubMed:16339847). Deubiquitinates HAS2 (PubMed:28604766). Deubiquitinates MAVs leading to maintain MAVS protein stability, resulting in increased production of type I interferons (IFN-I) and enhanced antiviral innate immune responses against viral infections (PubMed:39589880). Deubiquitinates RHEB in response to EGF signaling, promoting mTORC1 signaling (PubMed:30514904). May regulate mRNA splicing through deubiquitination of the U4 spliceosomal protein PRPF3 (PubMed:20595234). This may prevent its recognition by the U5 component PRPF8 thereby destabilizing interactions within the U4/U6.U5 snRNP (PubMed:20595234). May also play a role in the regulation of quality control in the ER (PubMed:16339847)

Protein Sequence

10 MAEGGGCRER 20 PDAETQKSEL 30 GPLMRTTLQR 40 GAQWYLIDSR 50 WFKQWKKYVG 60 FDSWDMYNVG 70 EHNLFPGPID 80 NSGLFSDPES 90 QTLKEHLIDE 100 LDYVLVPTEA 110 WNKLLNWYGC 120 VEGQQPIVRK 130 VVEHGLFVKH 140 CKVEVYLLEL 150 KLCENSDPTN 160 VLSCHFSKAD 170 TIATIEKEMR 180 KLFNIPAERE 190 TRLWNKYMSN 200 TYEQLSKLDN 210 TVQDAGLYQG 220 QVLVIEPQNE 230 DGTWPRQTLQ 240 SKSSTAPSRN 250 FTTSPKSSAS 260 PYSSVSASLI 270 ANGDSTSTCG 280 MHSSGVSRGG 290 SGFSASYNCQ 300 EPPSSHIQPG 310 LCGLGNLGNT 320 CFMNSALQCL 330 SNTAPLTDYF 340 LKDEYEAEIN 350 RDNPLGMKGE 360 IAEAYAELIK 370 QMWSGRDAHV 380 APRMFKTQVG 390 RFAPQFSGYQ 400 QQDSQELLAF 410 LLDGLHEDLN 420 RVKKKPYLEL 430 KDANGRPDAV 440 VAKEAWENHR 450 LRNDSVIVDT 460 FHGLFKSTLV 470 CPECAKVSVT 480 FDPFCYLTLP 490 LPLKKDRVME 500 VFLVPADPHC 510 RPTQYRVTVP 520 LMGAVSDLCE 530 ALSRLSGIAA 540 ENMVVADVYN 550 HRFHKIFQMD 560 EGLNHIMPRD 570 DIFVYEVCST 580 SVDGSECVTL 590 PVYFRERKSR 600 PSSTSSASAL 610 YGQPLLLSVP 620 KHKLTLESLY 630 QAVCDRISRY 640 VKQPLPDEFG 650 SSPLEPGACN 660 GSRNSCEGED 670 EEEMEHQEEG 680 KEQLSETEGS 690 GEDEPGNDPS 700 ETTQKKIKGQ 710 PCPKRLFTFS 720 LVNSYGTADI 730 NSLAADGKLL 740 KLNSRSTLAM 750 DWDSETRRLY 760 YDEQESEAYE 770 KHVSMLQPQK 780 KKKTTVALRD 790 CIELFTTMET 800 LGEHDPWYCP 810 NCKKHQQATK 820 KFDLWSLPKI 830 LVVHLKRFSY 840 NRYWRDKLDT 850 VVEFPIRGLN 860 MSEFVCNLSA 870 RPYVYDLIAV 880 SNHYGAMGVG 890 HYTAYAKNKL 900 NGKWYYFDDS 910 NVSLASEDQI 920 VTKAAYVLFY 930 QRRDDEFYKT 940 PSLSSSGSSD 950 GGTRPSSSQQ 960 GFGDDEACSM DTN

Gene Ontology

Classification GO ID Description
Cellular Component GO:0005737 cytoplasm
Cellular Component GO:0005829 cytosol
Cellular Component GO:0005764 lysosome
Cellular Component GO:0005654 nucleoplasm
Cellular Component GO:0005634 nucleus
Molecular Function GO:0031685 adenosine receptor binding
Molecular Function GO:0004843 cysteine-type deubiquitinase activity
Molecular Function GO:0042802 identical protein binding
Molecular Function GO:0046872 metal ion binding
Biological Process GO:0031397 negative regulation of protein ubiquitination
Biological Process GO:1904263 positive regulation of TORC1 signaling
Biological Process GO:0016579 protein deubiquitination
Biological Process GO:0034394 protein localization to cell surface
Biological Process GO:0006508 proteolysis
Biological Process GO:0031647 regulation of protein stability
Biological Process GO:0000244 spliceosomal tri-snRNP complex assembly

Reference

[1] Wu Q, Li Z, Gong T, Zheng X, Zhou X et al.. Porphyromonas gingivalis infection induces lysine lactylation reprogramming in human umbilical vein endothelial cells.. Front Cell Infect Microbiol 16:1706727. 2026. PMID: 41696360.