Search Results

Overview

Uniprot IDQ13127
Protein NameRE1-silencing transcription factor
Gene NameREST
OrganismHomo sapiens

Kla Sites from experimental identification

Position Flanking peptide
587 KKKTLKNKSSKKSSK

Function

Transcriptional repressor which binds neuron-restrictive silencer element (NRSE) and represses neuronal gene transcription in non-neuronal cells (PubMed:11741002, PubMed:11779185, PubMed:12399542, PubMed:26551668, PubMed:7697725, PubMed:7871435, PubMed:8568247). Restricts the expression of neuronal genes by associating with two distinct corepressors, SIN3A and RCOR1, which in turn recruit histone deacetylase to the promoters of REST-regulated genes (PubMed:10449787, PubMed:10734093). Mediates repression by recruiting the BHC complex at RE1/NRSE sites which acts by deacetylating and demethylating specific sites on histones, thereby acting as a chromatin modifier (By similarity). Transcriptional repression by REST-CDYL via the recruitment of histone methyltransferase EHMT2 may be important in transformation suppression (PubMed:19061646). Represses the expression of SRRM4 in non-neural cells to prevent the activation of neural-specific splicing events and to prevent production of REST isoform 3 (By similarity). Repressor activity may be inhibited by forming heterodimers with isoform 3, thereby preventing binding to NRSE or binding to corepressors and leading to derepression of target genes (PubMed:11779185). Also maintains repression of neuronal genes in neural stem cells, and allows transcription and differentiation into neurons by dissociation from RE1/NRSE sites of target genes (By similarity). Thereby is involved in maintaining the quiescent state of adult neural stem cells and preventing premature differentiation into mature neurons (PubMed:21258371). Plays a role in the developmental switch in synaptic NMDA receptor composition during postnatal development, by repressing GRIN2B expression and thereby altering NMDA receptor properties from containing primarily GRIN2B to primarily GRIN2A subunits (By similarity). Acts as a regulator of osteoblast differentiation (By similarity). Key repressor of gene expression in hypoxia; represses genes in hypoxia by direct binding to an RE1/NRSE site on their promoter regions (PubMed:27531581). May also function in stress resistance in the brain during aging; possibly by regulating expression of genes involved in cell death and in the stress response (PubMed:24670762). Repressor of gene expression in the hippocampus after ischemia by directly binding to RE1/NRSE sites and recruiting SIN3A and RCOR1 to promoters of target genes, thereby promoting changes in chromatin modifications and ischemia-induced cell death (By similarity). After ischemia, might play a role in repression of miR-132 expression in hippocampal neurons, thereby leading to neuronal cell death (By similarity). Negatively regulates the expression of SRRM3 in breast cancer cell lines (PubMed:26053433)

Protein Sequence

10 MATQVMGQSS 20 GGGGLFTSSG 30 NIGMALPNDM 40 YDLHDLSKAE 50 LAAPQLIMLA 60 NVALTGEVNG 70 SCCDYLVGEE 80 RQMAELMPVG 90 DNNFSDSEEG 100 EGLEESADIK 110 GEPHGLENME 120 LRSLELSVVE 130 PQPVFEASGA 140 PDIYSSNKDL 150 PPETPGAEDK 160 GKSSKTKPFR 170 CKPCQYEAES 180 EEQFVHHIRV 190 HSAKKFFVEE 200 SAEKQAKARE 210 SGSSTAEEGD 220 FSKGPIRCDR 230 CGYNTNRYDH 240 YTAHLKHHTR 250 AGDNERVYKC 260 IICTYTTVSE 270 YHWRKHLRNH 280 FPRKVYTCGK 290 CNYFSDRKNN 300 YVQHVRTHTG 310 ERPYKCELCP 320 YSSSQKTHLT 330 RHMRTHSGEK 340 PFKCDQCSYV 350 ASNQHEVTRH 360 ARQVHNGPKP 370 LNCPHCDYKT 380 ADRSNFKKHV 390 ELHVNPRQFN 400 CPVCDYAASK 410 KCNLQYHFKS 420 KHPTCPNKTM 430 DVSKVKLKKT 440 KKREADLPDN 450 ITNEKTEIEQ 460 TKIKGDVAGK 470 KNEKSVKAEK 480 RDVSKEKKPS 490 NNVSVIQVTT 500 RTRKSVTEVK 510 EMDVHTGSNS 520 EKFSKTKKSK 530 RKLEVDSHSL 540 HGPVNDEESS 550 TKKKKKVESK 560 SKNNSQEVPK 570 GDSKVEENKK 580 QNTCMKKSTK 590 KKTLKNKSSK 600 KSSKPPQKEP 610 VEKGSAQMDP 620 PQMGPAPTEA 630 VQKGPVQVEP 640 PPPMEHAQME 650 GAQIRPAPDE 660 PVQMEVVQEG 670 PAQKELLPPV 680 EPAQMVGAQI 690 VLAHMELPPP 700 METAQTEVAQ 710 MGPAPMEPAQ 720 MEVAQVESAP 730 MQVVQKEPVQ 740 MELSPPMEVV 750 QKEPVQIELS 760 PPMEVVQKEP 770 VKIELSPPIE 780 VVQKEPVQME 790 LSPPMGVVQK 800 EPAQREPPPP 810 REPPLHMEPI 820 SKKPPLRKDK 830 KEKSNMQSER 840 ARKEQVLIEV 850 GLVPVKDSWL 860 LKESVSTEDL 870 SPPSPPLPKE 880 NLREEASGDQ 890 KLLNTGEGNK 900 EAPLQKVGAE 910 EADESLPGLA 920 ANINESTHIS 930 SSGQNLNTPE 940 GETLNGKHQT 950 DSIVCEMKMD 960 TDQNTRENLT 970 GINSTVEEPV 980 SPMLPPSAVE 990 EREAVSKTAL 1000 ASPPATMAAN 1010 ESQEIDEDEG 1020 IHSHEGSDLS 1030 DNMSEGSDDS 1040 GLHGARPVPQ 1050 ESSRKNAKEA 1060 LAVKAAKGDF 1070 VCIFCDRSFR 1080 KGKDYSKHLN 1090 RHLVNVYYLE EAAQGQE

Gene Ontology

Classification GO ID Description
Cellular Component GO:0005737 cytoplasm
Biological Process GO:0043068 positive regulation of programmed cell death
Biological Process GO:1902459 positive regulation of stem cell population maintenance
Biological Process GO:0000381 regulation of alternative mRNA splicing, via spliceosome
Biological Process GO:0006355 regulation of DNA-templated transcription
Biological Process GO:0045667 regulation of osteoblast differentiation
Biological Process GO:0001666 response to hypoxia
Biological Process GO:0002931 response to ischemia
Biological Process GO:0035019 somatic stem cell population maintenance
Cellular Component GO:0005829 cytosol
Cellular Component GO:0005654 nucleoplasm
Cellular Component GO:0005634 nucleus
Cellular Component GO:0017053 transcription repressor complex
Molecular Function GO:0003682 chromatin binding
Molecular Function GO:0003700 DNA-binding transcription factor activity
Molecular Function GO:0001227 DNA-binding transcription repressor activity, RNA polymerase II-specific
Molecular Function GO:0042802 identical protein binding
Molecular Function GO:0000978 RNA polymerase II cis-regulatory region sequence-specific DNA binding
Molecular Function GO:0000979 RNA polymerase II core promoter sequence-specific DNA binding
Molecular Function GO:0061629 RNA polymerase II-specific DNA-binding transcription factor binding
Molecular Function GO:0000976 transcription cis-regulatory region binding
Molecular Function GO:0008270 zinc ion binding
Biological Process GO:0060088 auditory receptor cell stereocilium organization
Biological Process GO:0060379 cardiac muscle cell myoblast differentiation
Biological Process GO:0071257 cellular response to electrical stimulus
Biological Process GO:0071385 cellular response to glucocorticoid stimulus
Biological Process GO:0006338 chromatin remodeling
Biological Process GO:0050910 detection of mechanical stimulus involved in sensory perception of sound
Biological Process GO:0006351 DNA-templated transcription
Biological Process GO:0043922 host-mediated suppression of viral transcription
Biological Process GO:0099563 modification of synaptic structure
Biological Process GO:0032348 negative regulation of aldosterone biosynthetic process
Biological Process GO:2000798 negative regulation of amniotic stem cell differentiation
Biological Process GO:0045955 negative regulation of calcium ion-dependent exocytosis
Biological Process GO:2000065 negative regulation of cortisol biosynthetic process
Biological Process GO:2000706 negative regulation of dense core granule biogenesis
Biological Process GO:0045892 negative regulation of DNA-templated transcription
Biological Process GO:0010629 negative regulation of gene expression
Biological Process GO:0046676 negative regulation of insulin secretion
Biological Process GO:2000740 negative regulation of mesenchymal stem cell differentiation
Biological Process GO:1902894 negative regulation of miRNA transcription
Biological Process GO:0050768 negative regulation of neurogenesis
Biological Process GO:0045665 negative regulation of neuron differentiation
Biological Process GO:0000122 negative regulation of transcription by RNA polymerase II
Biological Process GO:0050877 nervous system process
Biological Process GO:0050885 neuromuscular process controlling balance
Biological Process GO:0097150 neuronal stem cell population maintenance
Biological Process GO:0045893 positive regulation of DNA-templated transcription
Biological Process GO:0010628 positive regulation of gene expression
Biological Process GO:0045666 positive regulation of neuron differentiation

Reference

[1] Cheng Z, Huang H, Li M, Chen Y. Proteomic analysis identifies PFKP lactylation in SW480 colon cancer cells.. iScience 27(1):108645. 2024 Jan 19. PMID: 38155775.