Overview
| Uniprot ID | Q13257 |
|---|
| Protein Name | Mitotic spindle assembly checkpoint protein MAD2A |
|---|
| Gene Name | MAD2L1 |
|---|
| Organism | Homo sapiens |
|---|
Kla Sites from experimental identification
| Position |
Flanking peptide |
| 111 |
IECDKTAKDDSAPRE |
| 192 |
SFTTTIHKVNSMVAY |
Function
Component of the mitotic checkpoint complex (MCC), which inhibits APC/C and delays chromosome segregation until all chromosomes are properly attached to the mitotic spindle (PubMed:10700282, PubMed:11804586, PubMed:15024386, PubMed:29162720, PubMed:27509861). The MCC, which consists of CDC20, MAD2L1/MAD2, BUB1B/BUBR1 and BUB3, is a key player of the spindle assembly checkpoint (PubMed:15020684, PubMed:27509861). The MCC-bound APC/C complex is inactive and this delays the metaphase/anaphase transition (PubMed:27509861). When the spindle assembly checkpoint is silenced, the MCC-APC/C complex recruits the E2 enzyme UBCH10 and triggers the auto-ubiquitination of CDC20 in the MCC, thereby reactivating APC/C for transition into anaphase (PubMed:27509861). In the closed conformation (C-MAD2) forms a heterotetrameric complex with MAD1L1 at unattached kinetochores during prometaphase, the complex recruits open conformation molecules of MAD2L1 (O-MAD2) and then promotes the conversion of O-MAD2 to C-MAD2 (PubMed:29162720)
Protein Sequence
10
MALQLSREQG
20
ITLRGSAEIV
30
AEFFSFGINS
40
ILYQRGIYPS
50
ETFTRVQKYG
60
LTLLVTTDLE
70
LIKYLNNVVE
80
QLKDWLYKCS
90
VQKLVVVISN
100
IESGEVLERW
110
QFDIECDKTA
120
KDDSAPREKS
130
QKAIQDEIRS
140
VIRQITATVT
150
FLPLLEVSCS
160
FDLLIYTDKD
170
LVVPEKWEES
180
GPQFITNSEE
190
VRLRSFTTTI
200
HKVNSMVAYK
IPVND
Gene Ontology
| Classification |
GO ID |
Description |
| Cellular Component |
GO:0005829 |
cytosol |
| Cellular Component |
GO:0000776 |
kinetochore |
| Cellular Component |
GO:0072686 |
mitotic spindle |
| Cellular Component |
GO:1990728 |
mitotic spindle assembly checkpoint MAD1-MAD2 complex |
| Cellular Component |
GO:0044615 |
nuclear pore nuclear basket |
| Cellular Component |
GO:0005634 |
nucleus |
| Cellular Component |
GO:0048471 |
perinuclear region of cytoplasm |
| Cellular Component |
GO:0000922 |
spindle pole |
| Molecular Function |
GO:0042802 |
identical protein binding |
| Molecular Function |
GO:0042803 |
protein homodimerization activity |
| Biological Process |
GO:0051301 |
cell division |
| Biological Process |
GO:0007094 |
mitotic spindle assembly checkpoint signaling |
| Biological Process |
GO:0045930 |
negative regulation of mitotic cell cycle |
| Biological Process |
GO:0042177 |
negative regulation of protein catabolic process |
| Biological Process |
GO:1904667 |
negative regulation of ubiquitin protein ligase activity |
| Biological Process |
GO:0090267 |
positive regulation of mitotic cell cycle spindle assembly checkpoint |
Reference
[1] He C, Zhang J, Bai X, Lu C, Zhang K. Lysine lactylation-based insight to understanding the characterization of cervical cancer.. Biochim Biophys Acta Mol Basis Dis 1870(7):167356. 2024 Oct. PMID: 39025375.