Search Results

Overview

Uniprot ID	Q13283
Protein Name	Ras GTPase-activating protein-binding protein 1
Gene Name	G3BP1
Organism	Homo sapiens

Kla Sites from experimental identification

Position	Flanking peptide
376	LRINSGGKLPNFGFV
64	GQKEIHRKVMSQNFT
76	NFTNCHTKIRHVDAH

Function

Protein involved in various processes, such as stress granule formation and innate immunity (PubMed:37672657, PubMed:20180778, PubMed:23279204, PubMed:30510222, PubMed:30804210). Plays an essential role in stress granule formation (PubMed:37672657, PubMed:20180778, PubMed:23279204, PubMed:32302570, PubMed:32302571, PubMed:32302572, PubMed:34739333, PubMed:35977029, PubMed:36183834, PubMed:36279435, PubMed:36692217, PubMed:37379838). Stress granules are membraneless compartments that store mRNAs and proteins, such as stalled translation pre-initiation complexes, in response to stress (PubMed:37672657, PubMed:20180778, PubMed:23279204, PubMed:27022092, PubMed:32302570, PubMed:32302571, PubMed:32302572, PubMed:36279435, PubMed:37379838). Promotes formation of stress granules phase-separated membraneless compartment by undergoing liquid-liquid phase separation (LLPS) upon unfolded RNA-binding: functions as a molecular switch that triggers RNA-dependent LLPS in response to a rise in intracellular free RNA concentrations (PubMed:32302570, PubMed:32302571, PubMed:32302572, PubMed:34739333, PubMed:36279435, PubMed:36692217). Also acts as an ATP- and magnesium-dependent helicase: unwinds DNA/DNA, RNA/DNA, and RNA/RNA substrates with comparable efficiency (PubMed:9889278). Acts unidirectionally by moving in the 5' to 3' direction along the bound single-stranded DNA (PubMed:9889278). Unwinds preferentially partial DNA and RNA duplexes having a 17 bp annealed portion and either a hanging 3' tail or hanging tails at both 5'- and 3'-ends (PubMed:9889278). Plays an essential role in innate immunity by promoting CGAS and RIGI activity (PubMed:30510222, PubMed:30804210). Participates in the DNA-triggered cGAS/STING pathway by promoting the DNA binding and activation of CGAS (PubMed:30510222). Triggers the condensation of cGAS, a process probably linked to the formation of membrane-less organelles (PubMed:34779554). Also enhances RIGI-induced type I interferon production probably by helping RIGI at sensing pathogenic RNA (PubMed:30804210). May also act as a phosphorylation-dependent sequence-specific endoribonuclease in vitro: Cleaves exclusively between cytosine and adenine and cleaves MYC mRNA preferentially at the 3'-UTR (PubMed:11604510)

Protein Sequence

10 MVMEKPSPLL 20 VGREFVRQYY 30 TLLNQAPDML 40 HRFYGKNSSY 50 VHGGLDSNGK 60 PADAVYGQKE 70 IHRKVMSQNF 80 TNCHTKIRHV 90 DAHATLNDGV 100 VVQVMGLLSN 110 NNQALRRFMQ 120 TFVLAPEGSV 130 ANKFYVHNDI 140 FRYQDEVFGG 150 FVTEPQEESE 160 EEVEEPEERQ 170 QTPEVVPDDS 180 GTFYDQAVVS 190 NDMEEHLEEP 200 VAEPEPDPEP 210 EPEQEPVSEI 220 QEEKPEPVLE 230 ETAPEDAQKS 240 SSPAPADIAQ 250 TVQEDLRTFS 260 WASVTSKNLP 270 PSGAVPVTGI 280 PPHVVKVPAS 290 QPRPESKPES 300 QIPPQRPQRD 310 QRVREQRINI 320 PPQRGPRPIR 330 EAGEQGDIEP 340 RRMVRHPDSH 350 QLFIGNLPHE 360 VDKSELKDFF 370 QSYGNVVELR 380 INSGGKLPNF 390 GFVVFDDSEP 400 VQKVLSNRPI 410 MFRGEVRLNV 420 EEKKTRAARE 430 GDRRDNRLRG 440 PGGPRGGLGG 450 GMRGPPRGGM 460 VQKPGFGVGR GLAPRQ

Gene Ontology

Classification	GO ID	Description
Cellular Component	GO:0005737	cytoplasm
Cellular Component	GO:0010494	cytoplasmic stress granule
Cellular Component	GO:0005829	cytosol
Cellular Component	GO:0005925	focal adhesion
Cellular Component	GO:0005634	nucleus
Cellular Component	GO:0043204	perikaryon
Molecular Function	GO:0005524	ATP binding
Molecular Function	GO:0016887	ATP hydrolysis activity
Molecular Function	GO:0003677	DNA binding
Molecular Function	GO:0003678	DNA helicase activity
Molecular Function	GO:0033677	DNA/RNA helicase activity
Molecular Function	GO:0004519	endonuclease activity
Molecular Function	GO:0140693	molecular condensate scaffold activity
Molecular Function	GO:0003729	mRNA binding
Molecular Function	GO:0043024	ribosomal small subunit binding
Molecular Function	GO:0003723	RNA binding
Molecular Function	GO:0003724	RNA helicase activity
Biological Process	GO:0051607	defense response to virus
Biological Process	GO:0045087	innate immune response
Biological Process	GO:0032481	positive regulation of type I interferon production
Biological Process	GO:0007265	Ras protein signal transduction
Biological Process	GO:0034063	stress granule assembly

Reference

[1] Yang Z, Yan C, Ma J, Peng P, Ren X et al.. Lactylome analysis suggests lactylation-dependent mechanisms of metabolic adaptation in hepatocellular carcinoma.. Nat Metab 5(1):61-79. 2023 Jan. PMID: 36593272.

[2] Cheng Z, Huang H, Li M, Chen Y. Proteomic analysis identifies PFKP lactylation in SW480 colon cancer cells.. iScience 27(1):108645. 2024 Jan 19. PMID: 38155775.

[3] He C, Zhang J, Bai X, Lu C, Zhang K. Lysine lactylation-based insight to understanding the characterization of cervical cancer.. Biochim Biophys Acta Mol Basis Dis 1870(7):167356. 2024 Oct. PMID: 39025375.

[4] Guo X, Ren X, Yan C, Huang H. Quantitative Proteomics Reveals the Role of Lysine Lactylation in Lenalidomide-Resistance in Multiple Myeloma Cells.. ACS Chem Biol 20(7):1728-1738. 2025 Jul 18. PMID: 40590393.