Overview
| Uniprot ID | Q13347 |
|---|
| Protein Name | Eukaryotic translation initiation factor 3 subunit I |
|---|
| Gene Name | EIF3I |
|---|
| Organism | Homo sapiens |
|---|
Kla Sites from experimental identification
| Position |
Flanking peptide |
| 17 |
ERSITQIKYNREGDL |
Function
Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis (PubMed:17581632, PubMed:25849773, PubMed:27462815). The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation (PubMed:17581632). The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression (PubMed:25849773)
Protein Sequence
10
MKPILLQGHE
20
RSITQIKYNR
30
EGDLLFTVAK
40
DPIVNVWYSV
50
NGERLGTYMG
60
HTGAVWCVDA
70
DWDTKHVLTG
80
SADNSCRLWD
90
CETGKQLALL
100
KTNSAVRTCG
110
FDFGGNIIMF
120
STDKQMGYQC
130
FVSFFDLRDP
140
SQIDNNEPYM
150
KIPCNDSKIT
160
SAVWGPLGEC
170
IIAGHESGEL
180
NQYSAKSGEV
190
LVNVKEHSRQ
200
INDIQLSRDM
210
TMFVTASKDN
220
TAKLFDSTTL
230
EHQKTFRTER
240
PVNSAALSPN
250
YDHVVLGGGQ
260
EAMDVTTTST
270
RIGKFEARFF
280
HLAFEEEFGR
290
VKGHFGPINS
300
VAFHPDGKSY
310
SSGGEDGYVR
320
IHYFDPQYFE
FEFEA
Gene Ontology
| Classification |
GO ID |
Description |
| Cellular Component |
GO:0005829 |
cytosol |
| Cellular Component |
GO:0016282 |
eukaryotic 43S preinitiation complex |
| Cellular Component |
GO:0033290 |
eukaryotic 48S preinitiation complex |
| Cellular Component |
GO:0005852 |
eukaryotic translation initiation factor 3 complex |
| Cellular Component |
GO:0071541 |
eukaryotic translation initiation factor 3 complex, eIF3m |
| Cellular Component |
GO:0070062 |
extracellular exosome |
| Cellular Component |
GO:0045202 |
synapse |
| Molecular Function |
GO:0003723 |
RNA binding |
| Molecular Function |
GO:0003743 |
translation initiation factor activity |
| Biological Process |
GO:0002183 |
cytoplasmic translational initiation |
| Biological Process |
GO:0001732 |
formation of cytoplasmic translation initiation complex |
| Biological Process |
GO:0006413 |
translational initiation |
Reference
[1] Wu Q, Li Z, Gong T, Zheng X, Zhou X et al.. Porphyromonas gingivalis infection induces lysine lactylation reprogramming in human umbilical vein endothelial cells.. Front Cell Infect Microbiol 16:1706727. 2026. PMID: 41696360.