Overview
| Uniprot ID | Q13418 |
|---|
| Protein Name | Scaffold protein ILK |
|---|
| Gene Name | ILK |
|---|
| Organism | Homo sapiens |
|---|
Kla Sites from experimental identification
| Position |
Flanking peptide |
| 184 |
PRNGTLNKHSGIDFK |
| 426 |
PHVCKLMKICMNEDP |
Function
Scaffold protein which mediates protein-protein interactions during a range of cellular events including focal adhesion assembly, cell adhesion and cell migration (PubMed:17420447, PubMed:20005845, PubMed:30367047, PubMed:32528174). Regulates integrin-mediated signal transduction by contributing to inside-out integrin activation (By similarity). Recruits PARVA and LIMS1/PITCH to form the heterotrimeric IPP (ILK-PINCH-PARVIN) complex which binds to F-actin via the C-terminal tail of LIMS1 and the N-terminal region of PARVA, promoting F-actin filament bundling, a process required to generate force for actin cytoskeleton reorganization and subsequent dynamic cell adhesion events such as cell spreading and migration (PubMed:30367047). Binding to PARVA promotes effective assembly of ILK into focal adhesions while PARVA-bound ILK can simultaneously engage integrin-beta cytoplasmic tails to mediate cell adhesion (PubMed:20005845). Plays a role with PARVG in promoting the cell adhesion and spreading of leukocytes (PubMed:16517730). Acts as an upstream effector of both AKT1/PKB and GSK3 (PubMed:9736715). Mediates trafficking of caveolae to the cell surface in an ITGB1-dependent manner by promoting the recruitment of IQGAP1 to the cell cortex which cooperates with its effector DIAPH1 to locally stabilize microtubules and allow stable insertion of caveolae into the plasma membrane (By similarity). Required for the maintenance of mitotic spindle integrity by promoting phosphorylation of TACC3 by AURKA (PubMed:18283114). Associates with chromatin and may act as a negative regulator of transcription when located in the nucleus (PubMed:17420447)
Protein Sequence
10
MDDIFTQCRE
20
GNAVAVRLWL
30
DNTENDLNQG
40
DDHGFSPLHW
50
ACREGRSAVV
60
EMLIMRGARI
70
NVMNRGDDTP
80
LHLAASHGHR
90
DIVQKLLQYK
100
ADINAVNEHG
110
NVPLHYACFW
120
GQDQVAEDLV
130
ANGALVSICN
140
KYGEMPVDKA
150
KAPLRELLRE
160
RAEKMGQNLN
170
RIPYKDTFWK
180
GTTRTRPRNG
190
TLNKHSGIDF
200
KQLNFLTKLN
210
ENHSGELWKG
220
RWQGNDIVVK
230
VLKVRDWSTR
240
KSRDFNEECP
250
RLRIFSHPNV
260
LPVLGACQSP
270
PAPHPTLITH
280
WMPYGSLYNV
290
LHEGTNFVVD
300
QSQAVKFALD
310
MARGMAFLHT
320
LEPLIPRHAL
330
NSRSVMIDED
340
MTARISMADV
350
KFSFQCPGRM
360
YAPAWVAPEA
370
LQKKPEDTNR
380
RSADMWSFAV
390
LLWELVTREV
400
PFADLSNMEI
410
GMKVALEGLR
420
PTIPPGISPH
430
VCKLMKICMN
440
EDPAKRPKFD
450
MIVPILEKMQ
DK
Gene Ontology
| Classification |
GO ID |
Description |
| Cellular Component |
GO:0005938 |
cell cortex |
| Cellular Component |
GO:0005813 |
centrosome |
| Cellular Component |
GO:0000785 |
chromatin |
| Cellular Component |
GO:0005737 |
cytoplasm |
| Cellular Component |
GO:0005829 |
cytosol |
| Cellular Component |
GO:0005925 |
focal adhesion |
| Cellular Component |
GO:0030027 |
lamellipodium |
| Cellular Component |
GO:0016020 |
membrane |
| Cellular Component |
GO:0005634 |
nucleus |
| Cellular Component |
GO:0005886 |
plasma membrane |
| Cellular Component |
GO:0030017 |
sarcomere |
| Molecular Function |
GO:0005524 |
ATP binding |
| Molecular Function |
GO:0005178 |
integrin binding |
| Molecular Function |
GO:0000287 |
magnesium ion binding |
| Molecular Function |
GO:0004672 |
protein kinase activity |
| Molecular Function |
GO:0019901 |
protein kinase binding |
| Molecular Function |
GO:0030674 |
protein-macromolecule adaptor activity |
| Molecular Function |
GO:0005102 |
signaling receptor binding |
| Biological Process |
GO:0051017 |
actin filament bundle assembly |
| Biological Process |
GO:0051639 |
actin filament network formation |
| Biological Process |
GO:0070836 |
caveola assembly |
| Biological Process |
GO:0030154 |
cell differentiation |
| Biological Process |
GO:0000902 |
cell morphogenesis |
| Biological Process |
GO:0007160 |
cell-matrix adhesion |
| Biological Process |
GO:0007229 |
integrin-mediated signaling pathway |
| Biological Process |
GO:0007052 |
mitotic spindle organization |
| Biological Process |
GO:0003151 |
outflow tract morphogenesis |
| Biological Process |
GO:0070527 |
platelet aggregation |
| Biological Process |
GO:0030513 |
positive regulation of BMP signaling pathway |
| Biological Process |
GO:0043123 |
positive regulation of canonical NF-kappaB signal transduction |
| Biological Process |
GO:0090263 |
positive regulation of canonical Wnt signaling pathway |
| Biological Process |
GO:0045893 |
positive regulation of DNA-templated transcription |
| Biological Process |
GO:0045669 |
positive regulation of osteoblast differentiation |
| Biological Process |
GO:0009967 |
positive regulation of signal transduction |
| Biological Process |
GO:1900026 |
positive regulation of substrate adhesion-dependent cell spreading |
| Biological Process |
GO:0072697 |
protein localization to cell cortex |
| Biological Process |
GO:0034446 |
substrate adhesion-dependent cell spreading |
| Biological Process |
GO:0033209 |
tumor necrosis factor-mediated signaling pathway |
Reference
[1] Yang Z, Yan C, Ma J, Peng P, Ren X et al.. Lactylome analysis suggests lactylation-dependent mechanisms of metabolic adaptation in hepatocellular carcinoma.. Nat Metab 5(1):61-79. 2023 Jan. PMID: 36593272.