Search Results

Overview

Uniprot ID	Q13423
Protein Name	NAD(P) transhydrogenase, mitochondrial
Gene Name	NNT
Organism	Homo sapiens

Kla Sites from experimental identification

Position	Flanking peptide
1036	VLEVWKSKQVIVMKR
1042	SKQVIVMKRSLGVGY
1071	MLLGDAKKTCDALQA
1079	TCDALQAKVRESYQK
117	GAQIQGAKEVLASDL
171	LNKLSQRKTTVLAMD
224	GQITAAGKVPPAKIL
279	EPLEVDLKESGEGQG
290	EGQGGYAKEMSKEFI
294	GYAKEMSKEFIEAEM
323	STALIPGKKAPVLFN
324	TALIPGKKAPVLFNK
331	KAPVLFNKEMIESMK
394	LYSNNITKLLKAISP
397	NNITKLLKAISPDKD
403	LKAISPDKDNFYFDV
433	TVVMKDGKVIFPAPT
442	IFPAPTPKNIPQGAP
451	IPQGAPVKQKTVAEL
453	QGAPVKQKTVAELEA
49	LWCKAPVKPGIPYKQ
55	VKPGIPYKQLTVGVP
70	KEIFQNEKRVALSPA
956	KMLTEQGKKVRFGIH

Function

The transhydrogenation between NADH and NADP is coupled to respiration and ATP hydrolysis and functions as a proton pump across the membrane (By similarity). May play a role in reactive oxygen species (ROS) detoxification in the adrenal gland (PubMed:22634753)

Protein Sequence

10 MANLLKTVVT 20 GCSCPLLSNL 30 GSCKGLRVKK 40 DFLRTFYTHQ 50 ELWCKAPVKP 60 GIPYKQLTVG 70 VPKEIFQNEK 80 RVALSPAGVQ 90 NLVKQGFNVV 100 VESGAGEASK 110 FSDDHYRVAG 120 AQIQGAKEVL 130 ASDLVVKVRA 140 PMVNPTLGVH 150 EADLLKTSGT 160 LISFIYPAQN 170 PELLNKLSQR 180 KTTVLAMDQV 190 PRVTIAQGYD 200 ALSSMANIAG 210 YKAVVLAANH 220 FGRFFTGQIT 230 AAGKVPPAKI 240 LIVGGGVAGL 250 ASAGAAKSMG 260 AIVRGFDTRA 270 AALEQFKSLG 280 AEPLEVDLKE 290 SGEGQGGYAK 300 EMSKEFIEAE 310 MKLFAQQCKE 320 VDILISTALI 330 PGKKAPVLFN 340 KEMIESMKEG 350 SVVVDLAAEA 360 GGNFETTKPG 370 ELYIHKGITH 380 IGYTDLPSRM 390 ATQASTLYSN 400 NITKLLKAIS 410 PDKDNFYFDV 420 KDDFDFGTMG 430 HVIRGTVVMK 440 DGKVIFPAPT 450 PKNIPQGAPV 460 KQKTVAELEA 470 EKAATITPFR 480 KTMSTASAYT 490 AGLTGILGLG 500 IAAPNLAFSQ 510 MVTTFGLAGI 520 VGYHTVWGVT 530 PALHSPLMSV 540 TNAISGLTAV 550 GGLALMGGHL 560 YPSTTSQGLA 570 ALAAFISSVN 580 IAGGFLVTQR 590 MLDMFKRPTD 600 PPEYNYLYLL 610 PAGTFVGGYL 620 AALYSGYNIE 630 QIMYLGSGLC 640 CVGALAGLST 650 QGTARLGNAL 660 GMIGVAGGLA 670 ATLGVLKPGP 680 ELLAQMSGAM 690 ALGGTIGLTI 700 AKRIQISDLP 710 QLVAAFHSLV 720 GLAAVLTCIA 730 EYIIEYPHFA 740 TDAAANLTKI 750 VAYLGTYIGG 760 VTFSGSLIAY 770 GKLQGLLKSA 780 PLLLPGRHLL 790 NAGLLAASVG 800 GIIPFMVDPS 810 FTTGITCLGS 820 VSALSAVMGV 830 TLTAAIGGAD 840 MPVVITVLNS 850 YSGWALCAEG 860 FLLNNNLLTI 870 VGALIGSSGA 880 ILSYIMCVAM 890 NRSLANVILG 900 GYGTTSTAGG 910 KPMEISGTHT 920 EINLDNAIDM 930 IREANSIIIT 940 PGYGLCAAKA 950 QYPIADLVKM 960 LTEQGKKVRF 970 GIHPVAGRMP 980 GQLNVLLAEA 990 GVPYDIVLEM 1000 DEINHDFPDT 1010 DLVLVIGAND 1020 TVNSAAQEDP 1030 NSIIAGMPVL 1040 EVWKSKQVIV 1050 MKRSLGVGYA 1060 AVDNPIFYKP 1070 NTAMLLGDAK 1080 KTCDALQAKV RESYQK

Gene Ontology

Classification	GO ID	Description
Cellular Component	GO:0016020	membrane
Cellular Component	GO:0005743	mitochondrial inner membrane
Cellular Component	GO:0005739	mitochondrion
Cellular Component	GO:0098803	respiratory chain complex
Molecular Function	GO:0051287	NAD binding
Molecular Function	GO:0003957	NAD(P)+ transhydrogenase (Si-specific) activity
Molecular Function	GO:0050661	NADP binding
Molecular Function	GO:0008750	proton-translocating NAD(P)+ transhydrogenase activity
Biological Process	GO:0045454	cell redox homeostasis
Biological Process	GO:0098869	cellular oxidant detoxification
Biological Process	GO:0032364	intracellular oxygen homeostasis
Biological Process	GO:0006740	NADPH regeneration
Biological Process	GO:0043066	negative regulation of apoptotic process
Biological Process	GO:1903285	positive regulation of hydrogen peroxide catabolic process
Biological Process	GO:0010918	positive regulation of mitochondrial membrane potential
Biological Process	GO:1902600	proton transmembrane transport
Biological Process	GO:0072593	reactive oxygen species metabolic process
Biological Process	GO:0033273	response to vitamin
Biological Process	GO:0006099	tricarboxylic acid cycle

Reference

[1] Yang Z, Yan C, Ma J, Peng P, Ren X et al.. Lactylome analysis suggests lactylation-dependent mechanisms of metabolic adaptation in hepatocellular carcinoma.. Nat Metab 5(1):61-79. 2023 Jan. PMID: 36593272.

[2] Hong H, Chen X, Wang H, Gu X, Yuan Y et al.. Global profiling of protein lysine lactylation and potential target modified protein analysis in hepatocellular carcinoma.. Proteomics 23(9):e2200432. 2023 May. PMID: 36625413.

[3] Shi CM, Wang QC, Li XL, Yang YH, Tang XY et al.. Global Profiling of Protein Lactylation in Human Hippocampi.. Proteomics Clin Appl 19(2):e202400061. 2025 Mar. PMID: 39610256.