Search Results

Overview

Uniprot IDQ13428
Protein NameTreacle protein
Gene NameTCOF1
OrganismHomo sapiens

Kla Sites from experimental identification

Position Flanking peptide
1034 AHPRIAPKASMAGAS
1044 MAGASSSKESSRISD
1054 SRISDGKKQEGPATQ
1064 GPATQVSKKNPASLP
1065 PATQVSKKNPASLPL
1078 PLTQAALKVLAQKAS
1083 ALKVLAQKASEAQPP
1120 QSTSVQAKGTNKLRK
1129 TNKLRKPKLPEVQQA
122 ADLPSSMKEKAKAET
1224 QASKATPKLDSSPSV
124 LPSSMKEKAKAETEK
1273 ASGTTPQKSRKPKKG
1348 GWESRKRKLSGDQPA
1366 PRSKKKKKLGAGEGG
1405 DVKEKKGKGSLGSQG
1437 GGDQSNPKSKKEKKK
1439 DQSNPKSKKEKKKSD
146 MPHPATGKTVANLLS
155 VANLLSGKSPRKSAE
159 LSGKSPRKSAEPSAN
224 SVKPAQVKASSVSTK
231 KASSVSTKESPARKA
237 TKESPARKAAPAPGK
244 KAAPAPGKVGDVTPQ
253 GDVTPQVKGGALPPA
261 GGALPPAKRAKKPEE
292 AGTRSQVKASEKILQ
296 SQVKASEKILQVRAA
308 RAASAPAKGTPGKGA
313 PAKGTPGKGATPAPP
322 ATPAPPGKAGAVASQ
363 AKALLQAKASGKTSQ
367 LQAKASGKTSQVGAA
379 GAASAPAKESPRKGA
384 PAKESPRKGAAPAPP
393 AAPAPPGKTGPAVAK
400 KTGPAVAKAQAGKRE
432 AQAKPSGKAPQVRAA
444 RAASAPAKESPRKGA
449 PAKESPRKGAAPAPP
458 AAPAPPRKTGPAAAQ
502 AQVKPLGKSPQVKPA
528 AGPVPPGKVGPATPS
586 KPTSSPAKGPPQKAG
591 PAKGPPQKAGPVAVQ
637 AQAKPALKIPQTKAC
64 SELGRKRKAEEDAAL
642 ALKIPQTKACPKKTN
655 TNTTASAKVAPVRVG
668 VGTQAPRKAGTATSP
716 AVTVGQAKSVGKGLQ
720 GQAKSVGKGLQVKAA
725 VGKGLQVKAASVPVK
732 KAASVPVKGSLGQGT
74 EDAALQAKKTRVSDP
746 TAPVLPGKTGPTVTQ
75 DAALQAKKTRVSDPI
755 GPTVTQVKAEKQEDS
791 SVKKTQAKANPAAAR
803 AARAPSAKGTISAPG
811 GTISAPGKVVTAAAQ
820 VTAAAQAKQRSPSKV
826 AKQRSPSKVKPPVRN
904 RAALAPAKESPRKGA
909 PAKESPRKGAAPTPP
918 AAPTPPGKTGPSAAQ

Function

Nucleolar protein that acts as a regulator of RNA polymerase I by connecting RNA polymerase I with enzymes responsible for ribosomal processing and modification (PubMed:12777385, PubMed:26399832). Required for neural crest specification: following monoubiquitination by the BCR(KBTBD8) complex, associates with NOLC1 and acts as a platform to connect RNA polymerase I with enzymes responsible for ribosomal processing and modification, leading to remodel the translational program of differentiating cells in favor of neural crest specification (PubMed:26399832)

Protein Sequence

10 MAEARKRREL 20 LPLIYHHLLR 30 AGYVRAAREV 40 KEQSGQKCFL 50 AQPVTLLDIY 60 THWQQTSELG 70 RKRKAEEDAA 80 LQAKKTRVSD 90 PISTSESSEE 100 EEEAEAETAK 110 ATPRLASTNS 120 SVLGADLPSS 130 MKEKAKAETE 140 KAGKTGNSMP 150 HPATGKTVAN 160 LLSGKSPRKS 170 AEPSANTTLV 180 SETEEEGSVP 190 AFGAAAKPGM 200 VSAGQADSSS 210 EDTSSSSDET 220 DVEGKPSVKP 230 AQVKASSVST 240 KESPARKAAP 250 APGKVGDVTP 260 QVKGGALPPA 270 KRAKKPEEES 280 ESSEEGSESE 290 EEAPAGTRSQ 300 VKASEKILQV 310 RAASAPAKGT 320 PGKGATPAPP 330 GKAGAVASQT 340 KAGKPEEDSE 350 SSSEESSDSE 360 EETPAAKALL 370 QAKASGKTSQ 380 VGAASAPAKE 390 SPRKGAAPAP 400 PGKTGPAVAK 410 AQAGKREEDS 420 QSSSEESDSE 430 EEAPAQAKPS 440 GKAPQVRAAS 450 APAKESPRKG 460 AAPAPPRKTG 470 PAAAQVQVGK 480 QEEDSRSSSE 490 ESDSDREALA 500 AMNAAQVKPL 510 GKSPQVKPAS 520 TMGMGPLGKG 530 AGPVPPGKVG 540 PATPSAQVGK 550 WEEDSESSSE 560 ESSDSSDGEV 570 PTAVAPAQEK 580 SLGNILQAKP 590 TSSPAKGPPQ 600 KAGPVAVQVK 610 AEKPMDNSES 620 SEESSDSADS 630 EEAPAAMTAA 640 QAKPALKIPQ 650 TKACPKKTNT 660 TASAKVAPVR 670 VGTQAPRKAG 680 TATSPAGSSP 690 AVAGGTQRPA 700 EDSSSSEESD 710 SEEEKTGLAV 720 TVGQAKSVGK 730 GLQVKAASVP 740 VKGSLGQGTA 750 PVLPGKTGPT 760 VTQVKAEKQE 770 DSESSEEESD 780 SEEAAASPAQ 790 VKTSVKKTQA 800 KANPAAARAP 810 SAKGTISAPG 820 KVVTAAAQAK 830 QRSPSKVKPP 840 VRNPQNSTVL 850 ARGPASVPSV 860 GKAVATAAQA 870 QTGPEEDSGS 880 SEEESDSEEE 890 AETLAQVKPS 900 GKTHQIRAAL 910 APAKESPRKG 920 AAPTPPGKTG 930 PSAAQAGKQD 940 DSGSSSEESD 950 SDGEAPAAVT 960 SAQVIKPPLI 970 FVDPNRSPAG 980 PAATPAQAQA 990 ASTPRKARAS 1000 ESTARSSSSE 1010 SEDEDVIPAT 1020 QCLTPGIRTN 1030 VVTMPTAHPR 1040 IAPKASMAGA 1050 SSSKESSRIS 1060 DGKKQEGPAT 1070 QVSKKNPASL 1080 PLTQAALKVL 1090 AQKASEAQPP 1100 VARTQPSSGV 1110 DSAVGTLPAT 1120 SPQSTSVQAK 1130 GTNKLRKPKL 1140 PEVQQATKAP 1150 ESSDDSEDSS 1160 DSSSGSEEDG 1170 EGPQGAKSAH 1180 TLGPTPSRTE 1190 TLVEETAAES 1200 SEDDVVAPSQ 1210 SLLSGYMTPG 1220 LTPANSQASK 1230 ATPKLDSSPS 1240 VSSTLAAKDD 1250 PDGKQEAKPQ 1260 QAAGMLSPKT 1270 GGKEAASGTT 1280 PQKSRKPKKG 1290 AGNPQASTLA 1300 LQSNITQCLL 1310 GQPWPLNEAQ 1320 VQASVVKVLT 1330 ELLEQERKKV 1340 VDTTKESSRK 1350 GWESRKRKLS 1360 GDQPAARTPR 1370 SKKKKKLGAG 1380 EGGEASVSPE 1390 KTSTTSKGKA 1400 KRDKASGDVK 1410 EKKGKGSLGS 1420 QGAKDEPEEE 1430 LQKGMGTVEG 1440 GDQSNPKSKK 1450 EKKKSDKRKK 1460 DKEKKEKKKK 1470 AKKASTKDSE 1480 SPSQKKKKKK KKTAEQTV

Gene Ontology

Classification GO ID Description
Cellular Component GO:0001650 fibrillar center
Cellular Component GO:0005730 nucleolus
Molecular Function GO:0046982 protein heterodimerization activity
Molecular Function GO:0030674 protein-macromolecule adaptor activity
Molecular Function GO:0003723 RNA binding
Molecular Function GO:0097110 scaffold protein binding
Biological Process GO:0014032 neural crest cell development
Biological Process GO:0014029 neural crest formation
Biological Process GO:0042790 nucleolar large rRNA transcription by RNA polymerase I
Biological Process GO:0006417 regulation of translation
Biological Process GO:0042254 ribosome biogenesis
Biological Process GO:0001501 skeletal system development

Reference

[1] Yang D, Yin J, Shan L, Yi X, Zhang W et al.. Identification of lysine-lactylated substrates in gastric cancer cells.. iScience 25(7):104630. 2022 Jul 15. PMID: 35800753.

[2] Yang Z, Yan C, Ma J, Peng P, Ren X et al.. Lactylome analysis suggests lactylation-dependent mechanisms of metabolic adaptation in hepatocellular carcinoma.. Nat Metab 5(1):61-79. 2023 Jan. PMID: 36593272.

[3] Hong H, Chen X, Wang H, Gu X, Yuan Y et al.. Global profiling of protein lysine lactylation and potential target modified protein analysis in hepatocellular carcinoma.. Proteomics 23(9):e2200432. 2023 May. PMID: 36625413.

[4] Yang YH, Wang QC, Kong J, Yang JT, Liu JF. Global profiling of lysine lactylation in human lungs.. Proteomics 23(15):e2200437. 2023 Aug. PMID: 37170646.

[5] Cheng Z, Huang H, Li M, Chen Y. Proteomic analysis identifies PFKP lactylation in SW480 colon cancer cells.. iScience 27(1):108645. 2024 Jan 19. PMID: 38155775.

[6] He C, Zhang J, Bai X, Lu C, Zhang K. Lysine lactylation-based insight to understanding the characterization of cervical cancer.. Biochim Biophys Acta Mol Basis Dis 1870(7):167356. 2024 Oct. PMID: 39025375.

[7] Bao Q, Wan N, He Z, Cao J, Yuan W et al.. Subcellular Proteomic Mapping of Lysine Lactylation.. J Am Soc Mass Spectrom 35(12):3221-3232. 2024 Dec 4. PMID: 39569522.

[8] Guo X, Ren X, Yan C, Huang H. Quantitative Proteomics Reveals the Role of Lysine Lactylation in Lenalidomide-Resistance in Multiple Myeloma Cells.. ACS Chem Biol 20(7):1728-1738. 2025 Jul 18. PMID: 40590393.

[9] He J, Lai T, Zhou Z, Yang H, Lei Z et al.. Multiomics profiling reveals the involvement of protein lactylation in nonhomologous end joining pathway conferring radioresistance in lung adenocarcinoma cell.. Sci Rep 15(1):24651. 2025 Jul 9. PMID: 40634431.

[10] Wu Q, Li Z, Gong T, Zheng X, Zhou X et al.. Porphyromonas gingivalis infection induces lysine lactylation reprogramming in human umbilical vein endothelial cells.. Front Cell Infect Microbiol 16:1706727. 2026. PMID: 41696360.