Search Results

Overview

Uniprot IDQ13464
Protein NameRho-associated protein kinase 1
Gene NameROCK1
OrganismHomo sapiens

Kla Sites from experimental identification

Position Flanking peptide
200 GFIHRDVKPDNMLLD
596 NRILENSKSQTDKDY

Function

Protein kinase which is a key regulator of the actin cytoskeleton and cell polarity (PubMed:10436159, PubMed:10652353, PubMed:11018042, PubMed:11283607, PubMed:17158456, PubMed:18573880, PubMed:19131646, PubMed:8617235, PubMed:9722579). Involved in regulation of smooth muscle contraction, actin cytoskeleton organization, stress fiber and focal adhesion formation, neurite retraction, cell adhesion and motility via phosphorylation of DAPK3, GFAP, LIMK1, LIMK2, MYL9/MLC2, TPPP, PFN1 and PPP1R12A (PubMed:10436159, PubMed:10652353, PubMed:11018042, PubMed:11283607, PubMed:17158456, PubMed:18573880, PubMed:19131646, PubMed:23093407, PubMed:23355470, PubMed:8617235, PubMed:9722579). Phosphorylates FHOD1 and acts synergistically with it to promote SRC-dependent non-apoptotic plasma membrane blebbing (PubMed:18694941). Phosphorylates JIP3 and regulates the recruitment of JNK to JIP3 upon UVB-induced stress (PubMed:19036714). Acts as a suppressor of inflammatory cell migration by regulating PTEN phosphorylation and stability (By similarity). Acts as a negative regulator of VEGF-induced angiogenic endothelial cell activation (PubMed:19181962). Required for centrosome positioning and centrosome-dependent exit from mitosis (By similarity). Plays a role in terminal erythroid differentiation (PubMed:21072057). Inhibits podocyte motility via regulation of actin cytoskeletal dynamics and phosphorylation of CFL1 (By similarity). Promotes keratinocyte terminal differentiation (PubMed:19997641). Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization (By similarity). May regulate closure of the eyelids and ventral body wall by inducing the assembly of actomyosin bundles (By similarity)

Protein Sequence

10 MSTGDSFETR 20 FEKMDNLLRD 30 PKSEVNSDCL 40 LDGLDALVYD 50 LDFPALRKNK 60 NIDNFLSRYK 70 DTINKIRDLR 80 MKAEDYEVVK 90 VIGRGAFGEV 100 QLVRHKSTRK 110 VYAMKLLSKF 120 EMIKRSDSAF 130 FWEERDIMAF 140 ANSPWVVQLF 150 YAFQDDRYLY 160 MVMEYMPGGD 170 LVNLMSNYDV 180 PEKWARFYTA 190 EVVLALDAIH 200 SMGFIHRDVK 210 PDNMLLDKSG 220 HLKLADFGTC 230 MKMNKEGMVR 240 CDTAVGTPDY 250 ISPEVLKSQG 260 GDGYYGRECD 270 WWSVGVFLYE 280 MLVGDTPFYA 290 DSLVGTYSKI 300 MNHKNSLTFP 310 DDNDISKEAK 320 NLICAFLTDR 330 EVRLGRNGVE 340 EIKRHLFFKN 350 DQWAWETLRD 360 TVAPVVPDLS 370 SDIDTSNFDD 380 LEEDKGEEET 390 FPIPKAFVGN 400 QLPFVGFTYY 410 SNRRYLSSAN 420 PNDNRTSSNA 430 DKSLQESLQK 440 TIYKLEEQLH 450 NEMQLKDEME 460 QKCRTSNIKL 470 DKIMKELDEE 480 GNQRRNLEST 490 VSQIEKEKML 500 LQHRINEYQR 510 KAEQENEKRR 520 NVENEVSTLK 530 DQLEDLKKVS 540 QNSQLANEKL 550 SQLQKQLEEA 560 NDLLRTESDT 570 AVRLRKSHTE 580 MSKSISQLES 590 LNRELQERNR 600 ILENSKSQTD 610 KDYYQLQAIL 620 EAERRDRGHD 630 SEMIGDLQAR 640 ITSLQEEVKH 650 LKHNLEKVEG 660 ERKEAQDMLN 670 HSEKEKNNLE 680 IDLNYKLKSL 690 QQRLEQEVNE 700 HKVTKARLTD 710 KHQSIEEAKS 720 VAMCEMEKKL 730 KEEREAREKA 740 ENRVVQIEKQ 750 CSMLDVDLKQ 760 SQQKLEHLTG 770 NKERMEDEVK 780 NLTLQLEQES 790 NKRLLLQNEL 800 KTQAFEADNL 810 KGLEKQMKQE 820 INTLLEAKRL 830 LEFELAQLTK 840 QYRGNEGQMR 850 ELQDQLEAEQ 860 YFSTLYKTQV 870 KELKEEIEEK 880 NRENLKKIQE 890 LQNEKETLAT 900 QLDLAETKAE 910 SEQLARGLLE 920 EQYFELTQES 930 KKAASRNRQE 940 ITDKDHTVSR 950 LEEANSMLTK 960 DIEILRRENE 970 ELTEKMKKAE 980 EEYKLEKEEE 990 ISNLKAAFEK 1000 NINTERTLKT 1010 QAVNKLAEIM 1020 NRKDFKIDRK 1030 KANTQDLRKK 1040 EKENRKLQLE 1050 LNQEREKFNQ 1060 MVVKHQKELN 1070 DMQAQLVEEC 1080 AHRNELQMQL 1090 ASKESDIEQL 1100 RAKLLDLSDS 1110 TSVASFPSAD 1120 ETDGNLPESR 1130 IEGWLSVPNR 1140 GNIKRYGWKK 1150 QYVVVSSKKI 1160 LFYNDEQDKE 1170 QSNPSMVLDI 1180 DKLFHVRPVT 1190 QGDVYRAETE 1200 EIPKIFQILY 1210 ANEGECRKDV 1220 EMEPVQQAEK 1230 TNFQNHKGHE 1240 FIPTLYHFPA 1250 NCDACAKPLW 1260 HVFKPPPALE 1270 CRRCHVKCHR 1280 DHLDKKEDLI 1290 CPCKVSYDVT 1300 SARDMLLLAC 1310 SQDEQKKWVT 1320 HLVKKIPKNP 1330 PSGFVRASPR 1340 TLSTRSTANQ 1350 SFRKVVKNTS GKTS

Gene Ontology

Classification GO ID Description
Biological Process GO:2000145 regulation of cell motility
Cellular Component GO:0032059 bleb
Cellular Component GO:0005814 centriole
Cellular Component GO:0005737 cytoplasm
Cellular Component GO:0010494 cytoplasmic stress granule
Cellular Component GO:0005856 cytoskeleton
Cellular Component GO:0005829 cytosol
Cellular Component GO:0005576 extracellular region
Cellular Component GO:0000139 Golgi membrane
Cellular Component GO:0030027 lamellipodium
Cellular Component GO:0005886 plasma membrane
Cellular Component GO:0001726 ruffle
Cellular Component GO:0098685 Schaffer collateral - CA1 synapse
Cellular Component GO:0034774 secretory granule lumen
Molecular Function GO:0005524 ATP binding
Molecular Function GO:0106310 protein serine kinase activity
Molecular Function GO:0004674 protein serine/threonine kinase activity
Molecular Function GO:0072518 Rho-dependent protein serine/threonine kinase activity
Molecular Function GO:0031267 small GTPase binding
Molecular Function GO:0048156 tau protein binding
Molecular Function GO:0050321 tau-protein kinase activity
Molecular Function GO:0008270 zinc ion binding
Biological Process GO:0030036 actin cytoskeleton organization
Biological Process GO:0031032 actomyosin structure organization
Biological Process GO:0003180 aortic valve morphogenesis
Biological Process GO:0006915 apoptotic process
Biological Process GO:0097746 blood vessel diameter maintenance
Biological Process GO:0030866 cortical actin cytoskeleton organization
Biological Process GO:0048598 embryonic morphogenesis
Biological Process GO:0001837 epithelial to mesenchymal transition
Biological Process GO:0035556 intracellular signal transduction
Biological Process GO:0007159 leukocyte cell-cell adhesion
Biological Process GO:0050900 leukocyte migration
Biological Process GO:0050901 leukocyte tethering or rolling
Biological Process GO:0022614 membrane to membrane docking
Biological Process GO:0000281 mitotic cytokinesis
Biological Process GO:0061157 mRNA destabilization
Biological Process GO:0051451 myoblast migration
Biological Process GO:1902992 negative regulation of amyloid precursor protein catabolic process
Biological Process GO:1902430 negative regulation of amyloid-beta formation
Biological Process GO:0016525 negative regulation of angiogenesis
Biological Process GO:1903347 negative regulation of bicellular tight junction assembly
Biological Process GO:0070168 negative regulation of biomineral tissue development
Biological Process GO:0051045 negative regulation of membrane protein ectodomain proteolysis
Biological Process GO:0032091 negative regulation of protein binding
Biological Process GO:0140058 neuron projection arborization
Biological Process GO:0031175 neuron projection development
Biological Process GO:0018105 peptidyl-serine phosphorylation
Biological Process GO:0090521 podocyte cell migration
Biological Process GO:1900223 positive regulation of amyloid-beta clearance
Biological Process GO:0010508 positive regulation of autophagy
Biological Process GO:0010613 positive regulation of cardiac muscle hypertrophy
Biological Process GO:1905205 positive regulation of connective tissue replacement
Biological Process GO:0051894 positive regulation of focal adhesion assembly
Biological Process GO:0010628 positive regulation of gene expression
Biological Process GO:0043410 positive regulation of MAPK cascade
Biological Process GO:0072659 protein localization to plasma membrane
Biological Process GO:0032956 regulation of actin cytoskeleton organization
Biological Process GO:0110061 regulation of angiotensin-activated signaling pathway
Biological Process GO:0030155 regulation of cell adhesion
Biological Process GO:1901888 regulation of cell junction assembly
Biological Process GO:0030334 regulation of cell migration
Biological Process GO:2000114 regulation of establishment of cell polarity
Biological Process GO:1903140 regulation of establishment of endothelial barrier
Biological Process GO:0051893 regulation of focal adhesion assembly
Biological Process GO:0045616 regulation of keratinocyte differentiation
Biological Process GO:0070507 regulation of microtubule cytoskeleton organization
Biological Process GO:0045664 regulation of neuron differentiation
Biological Process GO:0051492 regulation of stress fiber assembly
Biological Process GO:0090128 regulation of synapse maturation
Biological Process GO:1900242 regulation of synaptic vesicle endocytosis
Biological Process GO:1990776 response to angiotensin
Biological Process GO:0071559 response to transforming growth factor beta
Biological Process GO:0007266 Rho protein signal transduction
Biological Process GO:0007165 signal transduction
Biological Process GO:0006939 smooth muscle contraction

Reference

[1] Yang Z, Yan C, Ma J, Peng P, Ren X et al.. Lactylome analysis suggests lactylation-dependent mechanisms of metabolic adaptation in hepatocellular carcinoma.. Nat Metab 5(1):61-79. 2023 Jan. PMID: 36593272.

[2] He C, Zhang J, Bai X, Lu C, Zhang K. Lysine lactylation-based insight to understanding the characterization of cervical cancer.. Biochim Biophys Acta Mol Basis Dis 1870(7):167356. 2024 Oct. PMID: 39025375.